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Minor temperature shifts do not affect chromosomal ploidy but cause transcriptomic changes in Leishmania braziliensis promastigotes in vitro

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dc.creatorBallesteros, Nathaliaspa
dc.creatorVasquez, Nubia M.spa
dc.creatorPatino, Luz H.spa
dc.creatorCruz-Saavedra, Lissaspa
dc.creatorRamírez González, Juan Davidspa
dc.date.accessioned2020-06-11T13:21:46Z
dc.date.available2020-06-11T13:21:46Z
dc.date.created2020spa
dc.description.abstractBACKGROUND The leishmaniases are complex neglected diseases caused by protozoan parasites of the genus Leishmania. Leishmania braziliensis is the main etiological agent of cutaneous leishmaniasis in the New World. In recent studies, genomic changes such as chromosome and gene copy number variations (CNVs), as well as transcriptomic changes have been highlighted as mechanisms used by Leishmania species to adapt to stress situations. OBJECTIVES The aim of this study was to determine the effect of short-term minor temperature shifts in the genomic and transcriptomic responses of L. braziliensis promastigotes in vitro. METHODS Growth curves, genome and transcriptome sequencing of L. braziliensis promastigotes were conducted from cultures exposed to three different temperatures (24ºC, 28ºC and 30ºC) compared with the control temperature (26ºC). FINDINGS Our results showed a decrease in L. braziliensis proliferation at 30ºC, with around 3% of the genes showing CNVs at each temperature, and transcriptomic changes in genes encoding amastin surface-like proteins, heat shock proteins and transport proteins, which may indicate a direct response to temperature stress. MAIN CONCLUSIONS This study provides evidence that L. braziliensis promastigotes exhibit a decrease in cell density, and noticeable changes in the transcriptomic profiles. However, there were not perceptible changes at chromosome CNVs and only ~3% of the genes changed their copies in each treatment. © 2020, Fundacao Oswaldo Cruz. All rights reserved.eng
dc.format.mimetypeapplication/pdf
dc.identifier.issn0074-0276
dc.identifier.issn1678-8060
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/24900
dc.language.isoeng
dc.publisherFundacao Oswaldo Cruzspa
dc.relation.citationIssueNo. 2
dc.relation.citationTitleMemorias do Instituto Oswaldo Cruz
dc.relation.citationVolumeVol. 115
dc.relation.ispartofMemorias do Instituto Oswaldo Cruz, ISSN: 0074-0276;1678-8060, Vol.115, No.2 (2020); pp. -spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85084250124&doi=10.1590%2f0074-02760190431&partnerID=40&md5=09d427b2627b94487c04ece959ae4ccespa
dc.rights.accesRightsinfo:eu-repo/semantics/closedAccess
dc.rights.accesoBloqueado (Texto referencial)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordABC transporterspa
dc.subject.keywordAmastin-like proteinspa
dc.subject.keywordCopy number variationspa
dc.subject.keywordHeat shock proteinspa
dc.subject.keywordLeishmania braziliensisspa
dc.subject.keywordTemperature shiftspa
dc.subject.keywordTranscriptome profilespa
dc.subject.keywordtranscriptomespa
dc.subject.keywordadaptationspa
dc.subject.keywordanimalspa
dc.subject.keywordcopy number variationspa
dc.subject.keywordgene expression profilingspa
dc.subject.keywordgeneticsspa
dc.subject.keywordLeishmania braziliensisspa
dc.subject.keywordphysiologyspa
dc.subject.keywordtemperaturespa
dc.subject.keywordAdaptationspa
dc.subject.keywordPhysiologicalspa
dc.subject.keywordAnimalsspa
dc.subject.keywordDNA Copy Number Variationsspa
dc.subject.keywordGene Expression Profilingspa
dc.subject.keywordGenetic Profilespa
dc.subject.keywordLeishmania braziliensisspa
dc.subject.keywordTemperaturespa
dc.subject.keywordTranscriptomespa
dc.titleMinor temperature shifts do not affect chromosomal ploidy but cause transcriptomic changes in Leishmania braziliensis promastigotes in vitrospa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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