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Evaluación de la citotoxicidad de puntos de carbono (CD) en las líneas celulares tumorales U-87 Y MCF-7

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dc.contributor.advisorRodríguez Burbano, Diana Consuelo
dc.contributor.advisorOndo Méndez, Alejandro Oyono
dc.creatorRodríguez Rojas, Yoly Carolina
dc.creator.degreeIngeniero Biomédicospa
dc.creator.degreetypeFull timespa
dc.date.accessioned2021-06-16T20:48:19Z
dc.date.available2021-06-16T20:48:19Z
dc.date.created2021-05-28
dc.descriptionEl glioblastoma multiforme (GBM) es uno de los tipos de cáncer con mayor letalidad. El promedio de vida para todos los pacientes es de 12-18 meses después del diagnóstico y tratamiento. Los principales tratamientos para combatir el glioblastoma son: la cirugía y radioterapia. La cirugía principalmente utilizada para eliminar la mayor parte de la masa tumoral, ha tenido limitaciones dado a la característica invasiva que precede del GBM. La radioterapia (RT) ha tomado un papel crucial en terapias de GBM, siendo su objetivo detener la proliferación celular ocasionando rupturas en la cadena del ADN de las células cancerosas. Sin embargo, las células de GBM tienen un carácter radioresistente, limitando la efectividad de esta terapia. Los puntos de carbono (PC) son nanoestructuras esféricas, de alta biocompatibilidad, propiedades ópticas y fisicoquímicas que los hacen interesantes para aplicaciones dirigidas al aumentar la efectividad de la radioterapia. En estas aplicaciones, uno de los parámetros más importantes a establecer en los PC es su nivel de citotoxicidad. Por esto, el objetivo de este trabajo fue identificar el efecto citotóxico que tienen los puntos de carbono a base de ácido cítrico frente a células cancerosas de glioblastoma (U87) y células cancerosas de mama (MCF-7) por medio de dos ensayos de viabilidad MTT y Azul Tripán. Como resultado se obtuvieron puntos de carbono por medio de reacción microondas (bottom up) a partir de acido cítrico, etanol y N,N-dimetilformamida, emitiendo una fluorescencia de color azul bajo irradiación con luz ultravioleta de 365 nm. Se vió una alta viabilidad de las células U87 y MCF-7 frente a los PC sintetizados. Lo que da a entender que su baja citotoxicidad evidenciada en este trabajo, su facilidad para modular propiedades superficiales y su biocompatibilidad hacen que los PC sean potencialmente investigados para trabajos futuros.spa
dc.description.abstractGlioblastoma multiforme (GBM) is one of the most deadly cancers. The average life span for all patients is 12-18 months after diagnosis and treatment. The main treatments to combat glioblastoma are: surgery and radiation therapy. Surgery, mainly used to remove most of the tumor mass, has had limitations due to the invasive characteristic that precedes GBM. Radiotherapy (RT) has taken on a crucial role in GBM therapies, its objective being to stop cell proliferation by causing breaks in the DNA chain of cancer cells. However, GBM cells have a radioresistant character, limiting the effectiveness of this therapy. Carbon dots (PC) are spherical nanostructures, with high biocompatibility, optical and physicochemical properties that make them interesting for targeted applications by increasing the effectiveness of radiotherapy. In these applications, one of the most important parameters to establish in the PC is its level of cytotoxicity. Therefore, the objective of this work was to identify the cytotoxic effect that citric acid-based carbon spots have against glioblastoma cancer cells (U87) and breast cancer cells (MCF-7) by means of two viability assays. MTT and Azul Tripán. As a result, carbon points were obtained by means of microwave reaction (bottom up) from citric acid, ethanol and N, N-dimethylformamide, emitting a blue fluorescence under irradiation with 365 nm ultraviolet light. High viability of U87 and MCF-7 cells was seen against the synthesized PCs. This suggests that their low cytotoxicity evidenced in this work, their ease of modulating surface properties, and their biocompatibility make PCs potentially investigated for future work.eng
dc.format.extent38 pp.spa
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dc.identifier.doihttps://doi.org/10.48713/10336_31625
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/31625
dc.language.isospaspa
dc.publisherUniversidad del Rosariospa
dc.publisher.departmentEscuela de Medicina y Ciencias de la Saludspa
dc.publisher.programIngeniería Biomédicaspa
dc.rightsAtribución-NoComercial-SinDerivadas 2.5 Colombia*
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dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subjectGlioblastomaspa
dc.subjectPuntos de carbonospa
dc.subjectCitotoxicidadspa
dc.subjectAzul tripánspa
dc.subject.ddcIngeniería & operaciones afinesspa
dc.subject.lembCiencias médicas - Innovaciones tecnológicasspa
dc.subject.lembBioingenieríaspa
dc.titleEvaluación de la citotoxicidad de puntos de carbono (CD) en las líneas celulares tumorales U-87 Y MCF-7spa
dc.title.TranslatedTitleEvaluation of carbon point (CD) cytotoxicity in tumor cell lines U-87 and MCF-7spa
dc.typebachelorThesiseng
dc.type.documentTrabajo de gradospa
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersion
dc.type.spaTrabajo de gradospa
local.department.reportEscuela de Medicina y Ciencias de la Saludspa
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Pregrado en Ingeniería Biomédica