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Abnormality of Oct-1 DNA binding in T cells from Sjogren's syndrome patients

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dc.creatorFlescher, Eliezerspa
dc.creatorVela Roch, Normaspa
dc.creatorOgawa, Noriyoshispa
dc.creatorNakabayashi, Toruspa
dc.creatorEscalante, Agustinspa
dc.creatorAnaya, Juan-Manuel
dc.date.accessioned2020-08-19T14:44:30Z
dc.date.available2020-08-19T14:44:30Z
dc.date.created1996spa
dc.description.abstractPrimary Sjögren's syndrome (SS) is an autoimmune rheumatic disease characterized by T cell hypoactivity. To understand the diminished T cell response to activation signals, we measured nucleoprotein DNA?binding activities regulating gene expression during T cell activation using the electrophoretic mobility shift assay. Peripheral blood lymphocytes from 9/19 SS patients were found to be defective in their ability to bind an octomer sequence (Oct?1). This Oct?1?binding phenotype remained stable in culture for up to 3 days prior to activation. This abnormality was not seen in resting T cells nor T cells from patients with systemic lupus erythematosus, rheumatoid arthritis (RA), or SS accompanied by RA. The SS Oct?1 DNA?binding abnormality correlated significantly with an inability of cells to exit the G0/G1 cell cycle phase when stimulated in vitro . Importantly, nucleoprotein extracts showing decreased DNA?binding activity had normal amounts of Oct?1 proteins as determined by immunoprecipitation, implying a functional defect in the Oct?1 protein. Moreover, defective DNA binding was corrected by treatment with acid phosphatase.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1002/eji.1830260906
dc.identifier.issnISSN: 0014-2980
dc.identifier.issnEISSN: 1521-4141
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/27897
dc.language.isoengspa
dc.publisherJohn Wiley & Sonsspa
dc.relation.citationEndPage2011
dc.relation.citationIssueNo. 9
dc.relation.citationStartPage2006
dc.relation.citationTitleEuropean Journal of Immunology
dc.relation.citationVolumeVol. 26
dc.relation.ispartofEuropean Journal of Immunology, ISSN: 0014-2980;EISSN:1521-4141, Vol.26, No.9 (September, 1996); pp. 2006-2011spa
dc.relation.urihttps://onlinelibrary.wiley.com/doi/abs/10.1002/eji.1830260906spa
dc.rights.accesRightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.accesoRestringido (Acceso a grupos específicos)spa
dc.sourceEuropean Journal of Immunologyspa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.keywordPrimary Sjögren's syndromespa
dc.subject.keywordT cell hypoactivityspa
dc.titleAbnormality of Oct-1 DNA binding in T cells from Sjogren's syndrome patientsspa
dc.title.TranslatedTitleAnormalidad de la unión al ADN de Oct-1 en las células T de pacientes con síndrome de Sjogrenspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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