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Characterizing PvARP, a novel Plasmodium vivax antigen

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dc.audienceComunidad Rosaristaspa
dc.creatorMoreno Pérez, Darwin A
dc.creatorSaldarriaga, Ambar
dc.creatorPatarroyo, Manuel A.
dc.creator.googleMoreno Pérez, Darwin A
dc.creator.googleSaldarriaga, Ambar
dc.creator.googlePatarroyo, Manuel A.
dc.date.accessioned2014-08-13T15:43:54Z
dc.date.available2014-08-13T15:43:54Z
dc.date.created2013
dc.date.issued2013
dc.description.abstractBackground Plasmodium vivax continues to be the most widely distributed malarial parasite species in tropical and sub-tropical areas, causing high morbidity indices around the world. Better understanding of the proteins used by the parasite during the invasion of red blood cells is required to obtain an effective vaccine against this disease. This study describes characterizing the P. vivax asparagine-rich protein (PvARP) and examines its antigenicity in natural infection. Methods The target gene in the study was selected according to a previous in silico analysis using profile hidden Markov models which identified P. vivax proteins that play a possible role in invasion. Transcription of the arp gene in the P. vivax VCG-1 strain was here evaluated by RT-PCR. Specific human antibodies against PvARP were used to confirm protein expression by Western blot as well as its subcellular localization by immunofluorescence. Recognition of recombinant PvARP by sera from P. vivax-infected individuals was evaluated by ELISA. Results VCG-1 strain PvARP is a 281-residue-long molecule, which is encoded by a single exon and has an N-terminal secretion signal, as well as a tandem repeat region. This protein is expressed in mature schizonts and is located on the surface of merozoites, having an apparent accumulation towards their apical pole. Sera from P. vivax-infected patients recognized the recombinant, thereby suggesting that this protein is targeted by the immune response during infection.eng
dc.format.mediumRecurso electrónicospa
dc.format.mimetypeapplication/pdf
dc.format.tipoDocumentospa
dc.identifier.issnISSN:1475-2875
dc.identifier.urihttp://repository.urosario.edu.co/handle/10336/8826
dc.language.isoeng
dc.publisherUniversidad del Rosariospa
dc.relation.citationIssueNo. 165
dc.relation.citationTitleMalaria Journal
dc.relation.citationVolumeVol. 12
dc.relation.ispartofMalaria Journal ISSN 1475-2875 V. 12 N. 165 2013spa
dc.relation.urihttp://www.malariajournal.com/content/pdf/1475-2875-12-165.pdf
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto completo)spa
dc.rights.licenciaEL AUTOR, manifiesta que la obra objeto de la presente autorización es original y la realizó sin violar o usurpar derechos de autor de terceros, por lo tanto la obra es de exclusiva autoría y tiene la titularidad sobre la misma.spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.ddcEnfermedades
dc.subject.decsPlasmodiumspa
dc.subject.decsMalariaspa
dc.subject.decsAntígenosspa
dc.subject.decsEnfermedades infecciosasspa
dc.subject.keywordPlasmodium vivaxeng
dc.subject.keywordProteineng
dc.subject.keywordInvasioneng
dc.subject.keywordAntigenicityeng
dc.subject.keywordVaccineeng
dc.titleCharacterizing PvARP, a novel Plasmodium vivax antigenspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersion
dc.type.spaArtículospa
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