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A Trypanosoma cruzi Genome Tandem Repetitive Satellite DNA Sequence as a Molecular Marker for a LAMP Assay for Diagnosing Chagas' Disease

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dc.creatorOrdóñez, Diegospa
dc.creatorFernández-Soto, Pedrospa
dc.creatorFernández-Martín, Ana Mspa
dc.creatorCrego-Vicente, Beatrizspa
dc.creatorFebrer-Sendra, Begoñaspa
dc.creatorDiego, Juan García-Bernaltspa
dc.creatorVicente, Belénspa
dc.creatorLópez-Abán, Juliospa
dc.creatorBelhassen-García, Moncefspa
dc.creatorMuro, Antoniospa
dc.creatorPatarroyo, Manuel A.
dc.date.accessioned2020-05-26T00:06:08Z
dc.date.available2020-05-26T00:06:08Z
dc.date.created2020spa
dc.description.abstractChagas' disease is a neglected tropical disease caused by Trypanosoma cruzi which is endemic throughout Latin America and is spread by worldwide migration. Diagnosis is currently limited to serological and molecular techniques having variations regarding their sensitivity and specificity. This work was aimed at developing a new sensitive, applicable, and cost-effective molecular diagnosis technique for loop-mediated isothermal amplification-based detection of T. cruzi (Tc-LAMP). The results led to determining a highly homologous satellite repeat region (231 bp) among parasite strains as a molecular marker for diagnosing the disease. Tc-LAMP was performed correctly for detecting parasite DNA (5 fg for the CL Brener strain and 50 fg for the DM28, TcVI, and TcI strains). Assay results proved negative for DNA from 16 helminth species and 7 protozoa, including Leishmania spp. Tc-LAMP based on the highly repeated T. cruzi satellite region is thus proposed as an important alternative for diagnosing T. cruzi infection, overcoming other methods' limitations such as their analytic capability, speed, and requiring specialized equipment or highly trained personnel. Tc-LAMP could be easily adapted for point-of-care testing in areas having limited resources. © 2020 Diego Ordóñez et al.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1155/2020/8074314
dc.identifier.issn2780240
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23860
dc.language.isoengspa
dc.publisherHindawi Limitedspa
dc.relation.citationTitleDisease Markers
dc.relation.citationVolumeVol. 2020
dc.relation.ispartofDisease Markers, ISSN:2780240, Vol.2020,(2020)spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85081197087&doi=10.1155%2f2020%2f8074314&partnerID=40&md5=177cc2dfe20c680795dee37aeb04ee39spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordCalcium binding proteinspa
dc.subject.keywordRibosome rnaspa
dc.subject.keywordSatellite dnaspa
dc.subject.keywordArticlespa
dc.subject.keywordChagas diseasespa
dc.subject.keywordColorimetryspa
dc.subject.keywordControlled studyspa
dc.subject.keywordCost effectiveness analysisspa
dc.subject.keywordCross reactionspa
dc.subject.keywordDna contaminationspa
dc.subject.keywordDna templatespa
dc.subject.keywordHumanspa
dc.subject.keywordIn vitro studyspa
dc.subject.keywordLimit of detectionspa
dc.subject.keywordLoop mediated isothermal amplificationspa
dc.subject.keywordMolecular diagnosisspa
dc.subject.keywordNonhumanspa
dc.subject.keywordNucleotide sequencespa
dc.subject.keywordPoint of care testingspa
dc.subject.keywordReal time polymerase chain reactionspa
dc.subject.keywordTrypanosoma cruzispa
dc.titleA Trypanosoma cruzi Genome Tandem Repetitive Satellite DNA Sequence as a Molecular Marker for a LAMP Assay for Diagnosing Chagas' Diseasespa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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