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Differential PbP27 expression in the yeast and mycelial forms of the Paracoccidioides brasiliensis species complex

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dc.creatorGarcía Blanco S.spa
dc.creatorMuñoz J.F.spa
dc.creatorTorres I.spa
dc.creatorDíez Posada S.spa
dc.creatorGómez B.L.spa
dc.creatorMcEwen J.G.spa
dc.creatorRestrepo S.spa
dc.creatorGarcía A.M.spa
dc.date.accessioned2020-05-26T00:08:44Z
dc.date.available2020-05-26T00:08:44Z
dc.date.created2011spa
dc.description.abstractp27 is an antigenic protein produced by Paracoccidioides brasiliensis, the etiologic agent of paracoccidioidomycosis (PCM). Despite its unknown function, it has been suggested as a putative virulence factor, proposed as a suitable target for the design of diagnostic tools and vaccines, and considered as an enhancer in antifungal treatment of PCM. We evaluated sequence polymorphisms of PbP27 gene sequence among isolates, finding some polymorphisms associated with the isolates' phylogenetic origin. In order to determine if there was a differential expression pattern between morphological states and among isolates, we also evaluated PbP27 expression, at transcriptional and translational levels, in mycelia and yeast cultures in 14 isolates belonging to the P. brasiliensis species complex (S1, PS2, PS3, and 'Pb01-like', proposed to be named Paracoccidioides lutzii) by two techniques, real time RT-PCR (RT-qPCR) and protein dot blot. For the latter, four protein extracts from different cell localizations (SDS or ?-mercaptoethanol, cytoplasmic and extracellular proteins) were analyzed for each isolate. p27 was present in the four extracts evaluated, mainly in the SDS extract, corresponding to an extract containing proteins loosely attached to the cell wall. This information correlates with immunohistochemical analysis, where positive staining of the yeasts' cell wall was observed. We found that p27 was present in all isolates, mainly in the yeast form. This pattern was corroborated by RT-qPCR results, with higher expression levels found in the yeast form for most of the isolates. The results provide new insights into the expression patterns of this protein, and further characterize it in view of potential uses as a diagnostic and/or therapeutic tool. © 2011 Elsevier Inc.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.fgb.2011.09.001
dc.identifier.issn10960937
dc.identifier.issn10871845
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/24108
dc.language.isoengspa
dc.relation.citationEndPage1095
dc.relation.citationIssueNo. 12
dc.relation.citationStartPage1087
dc.relation.citationTitleFungal Genetics and Biology
dc.relation.citationVolumeVol. 48
dc.relation.ispartofFungal Genetics and Biology, ISSN:10960937, 10871845, Vol.48, No.12 (2011); pp. 1087-1095spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-82555165930&doi=10.1016%2fj.fgb.2011.09.001&partnerID=40&md5=c9b795c7ff523189d9a5a31808fae26bspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordCytoplasm proteinspa
dc.subject.keywordpolyacrylamide geleng
dc.subject.keywordMercaptoethanolspa
dc.subject.keywordProtein p27spa
dc.subject.keywordAntigen expressionspa
dc.subject.keywordArticlespa
dc.subject.keywordCell wallspa
dc.subject.keywordCellular distributionspa
dc.subject.keywordControlled studyspa
dc.subject.keywordDna polymorphismspa
dc.subject.keywordDot hybridizationspa
dc.subject.keywordFungal genespa
dc.subject.keywordFungal geneticsspa
dc.subject.keywordFungal phenomena and functionsspa
dc.subject.keywordFungal strainspa
dc.subject.keywordFungus culturespa
dc.subject.keywordFungus isolatespa
dc.subject.keywordGene sequencespa
dc.subject.keywordGenetic transcriptionspa
dc.subject.keywordImmunohistochemistryspa
dc.subject.keywordMyceliumspa
dc.subject.keywordNonhumanspa
dc.subject.keywordNucleotide sequencespa
dc.subject.keywordParacoccidioidesspa
dc.subject.keywordParacoccidioides brasiliensisspa
dc.subject.keywordParacoccidioides brasiliensis species complexspa
dc.subject.keywordParacoccidioides brasiliensis type pb01 likespa
dc.subject.keywordParacoccidioides brasiliensis type ps2spa
dc.subject.keywordParacoccidioides brasiliensis type ps3spa
dc.subject.keywordParacoccidioides brasiliensis type s1spa
dc.subject.keywordParacoccidioides lutziispa
dc.subject.keywordPbp27 genespa
dc.subject.keywordPhylogenyspa
dc.subject.keywordPolyacrylamide gel electrophoresisspa
dc.subject.keywordPriority journalspa
dc.subject.keywordProtein localizationspa
dc.subject.keywordReal time polymerase chain reactionspa
dc.subject.keywordReverse transcription polymerase chain reactionspa
dc.subject.keywordRna translationspa
dc.subject.keywordSequence analysisspa
dc.subject.keywordSouth american blastomycosisspa
dc.subject.keywordStrain differencespa
dc.subject.keywordWestern blottingspa
dc.subject.keywordYeastspa
dc.subject.keywordAntigenseng
dc.subject.keywordBlottingeng
dc.subject.keywordCell wallspa
dc.subject.keywordElectrophoresiseng
dc.subject.keywordFungal proteinsspa
dc.subject.keywordGene expression regulationeng
dc.subject.keywordGeneseng
dc.subject.keywordImmunohistochemistryspa
dc.subject.keywordParacoccidioidesspa
dc.subject.keywordPolymorphismeng
dc.subject.keywordReal-time polymerase chain reactionspa
dc.subject.keywordReverse transcriptase polymerase chain reactionspa
dc.subject.keywordSequence analysiseng
dc.subject.keywordYeastsspa
dc.subject.keywordParacoccidioidesspa
dc.subject.keywordParacoccidioides brasiliensisspa
dc.subject.keywordDimorphismspa
dc.subject.keywordDot blotspa
dc.subject.keywordP27 antigenspa
dc.subject.keywordParacoccidioides brasiliensisspa
dc.subject.keywordParacoccidioidomycosisspa
dc.subject.keywordQpcrspa
dc.subject.keywordWestern blotspa
dc.titleDifferential PbP27 expression in the yeast and mycelial forms of the Paracoccidioides brasiliensis species complexspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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