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PvRON2, a new Plasmodium vivax rhoptry neck antigen

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dc.audienceComunidad Rosaristaspa
dc.creatorArévalo Pinzón, Gabriela
dc.creatorCurtidor, Hernando
dc.creatorPatiño, Liliana C
dc.creatorPatarroyo, Manuel A.
dc.creator.googleArévalo Pinzón, Gabriela
dc.creator.googleCurtidor, Hernando
dc.creator.googlePatiño, Liliana C
dc.creator.googlePatarroyo, Manuel A.
dc.date.accessioned2014-08-13T15:51:00Z
dc.date.available2014-08-13T15:51:00Z
dc.date.created2011
dc.date.issued2011
dc.description.abstractBackground: Rhoptries are specialized organelles from parasites belonging to the phylum Apicomplexa; they secrete their protein content during invasion of host target cells and are sorted into discrete subcompartments within rhoptry neck or bulb. This distribution is associated with these proteins’ role in tight junction (TJ) and parasitophorous vacuole (PV) formation, respectively. Methods: Plasmodium falciparum RON2 amino acid sequence was used as bait for screening the codifying gene for the homologous protein in the Plasmodium vivax genome. Gene synteny, as well as identity and similarity values, were determined for ron2 and its flanking genes among P. falciparum, P. vivax and other malarial parasite genomes available at PlasmoDB and Sanger Institute databases. Pvron2 gene transcription was determined by RT-PCR of cDNA obtained from the P. vivax VCG-1 strain. Protein expression and localization were assessed by Western blot and immunofluorescence using polyclonal anti-PvRON2 antibodies. Co-localization was confirmed using antibodies directed towards specific microneme and rhoptry neck proteins. Results and discussion: The first P. vivax rhoptry neck protein (named here PvRON2) has been identified in this study. PvRON2 is a 2,204 residue-long protein encoded by a single 6,615 bp exon containing a hydrophobic signal sequence towards the amino-terminus, a transmembrane domain towards the carboxy-terminus and two coiled coil a-helical motifs; these are characteristic features of several previously described vaccine candidates against malaria. This protein also contains two tandem repeats within the interspecies variable sequence possibly involved in evading a host’s immune system. PvRON2 is expressed in late schizonts and localized in rhoptry necks similar to what has been reported for PfRON2, which suggests its participation during target cell invasion. Conclusions: The identification and partial characterization of the first P. vivax rhoptry neck protein are described in the present study. This protein is homologous to PfRON2 which has previously been shown to be associated with PfAMA-1, suggesting a similar role for PvRON2.eng
dc.format.mediumRecurso electrónicospa
dc.format.mimetypeapplication/pdf
dc.format.tipoDocumentospa
dc.identifier.issnISSN:1475-2875
dc.identifier.urihttp://repository.urosario.edu.co/handle/10336/8827
dc.language.isoeng
dc.publisherUniversidad del Rosariospa
dc.relation.citationIssueNo. 60
dc.relation.citationTitleMalaria Journal
dc.relation.citationVolumeVol. 10
dc.relation.ispartofMalaria Journal ISSN 1475-2875 V. 10 N. 60 2011spa
dc.relation.urihttp://www.malariajournal.com/content/pdf/1475-2875-10-60.pdf
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto completo)spa
dc.rights.licenciaEL AUTOR, manifiesta que la obra objeto de la presente autorización es original y la realizó sin violar o usurpar derechos de autor de terceros, por lo tanto la obra es de exclusiva autoría y tiene la titularidad sobre la misma.spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.ddcEnfermedades
dc.subject.decsParásitosspa
dc.subject.decsPlasmodiumspa
dc.subject.decsGenéticaspa
dc.titlePvRON2, a new Plasmodium vivax rhoptry neck antigenspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersion
dc.type.spaArtículospa
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