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Analytical performance of a loop-mediated isothermal amplification assay for leishmania DNA detection in sandflies and direct smears of patients with cutaneous leishmaniasis

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dc.creatorLeón, Cielo Mspa
dc.creatorMuñoz, Marinaspa
dc.creatorTabares, Juan Hspa
dc.creatorHernandez, Carolinaspa
dc.creatorFlorez, Carolinaspa
dc.creatorAyala, Martha Sspa
dc.creatorRamírez, Juan David
dc.date.accessioned2020-05-26T00:10:13Z
dc.date.available2020-05-26T00:10:13Z
dc.date.created2018spa
dc.description.abstractLoop-mediated isothermal amplification (LAMP) is ideal for the detection of Leishmania DNA as it is a quick and easy-to-perform test that does not require complex or sophisticated equipment or infrastructure. However, the application of this technique in the detection of Leishmania DNA has not been comprehensively analyzed to date (analytical validation). Our objective was to evaluate the sensitivity and analytical specificity (anticipated reportable range [ARR], the limit of detection [LoD], and accuracy) of LAMP targeting the 18S rRNA gene in the diagnosis of six New World Leishmania species. We then applied the validated LAMP assay across 50 samples of sandflies and 50 direct smears from a recent outbreak of cutaneous leishmaniasis in Colombia to determine its diagnostic performance. The LAMP assay exclusively amplified the DNA of Leishmania spp., and an ARR of between 1 × 10 4 and 1 × 10 -2 equivalent parasites/mL was determined. An LoD of 1 × 10 -2 equivalent parasites/mL was established and there was no statistically significant variation in terms of accuracy. Finally, a sensitivity of 100% in direct smears and sandflies samples was calculated and a specificity of 90.9% for direct smears using microscopy as reference and 96.8% for sandflies using real-time polymerase chain reaction as reference were determined. To our knowledge, this is the first attempt to analytically validate a LAMP test to detect Leishmania DNA, which showed good diagnostic potential from sandflies and direct smear samples. Copyright © 2018 by The American Society of Tropical Medicine and Hygiene.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.4269/ajtmh.17-0808
dc.identifier.issn29637
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/24218
dc.language.isoengspa
dc.publisherAmerican Society of Tropical Medicine and Hygienespa
dc.relation.citationEndPage1331
dc.relation.citationIssueNo. 5
dc.relation.citationStartPage1325
dc.relation.citationTitleAmerican Journal of Tropical Medicine and Hygiene
dc.relation.citationVolumeVol. 98
dc.relation.ispartofAmerican Journal of Tropical Medicine and Hygiene, ISSN:29637, Vol.98, No.5 (2018); pp. 1325-1331spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85046905773&doi=10.4269%2fajtmh.17-0808&partnerID=40&md5=a0d2c1ca05381399d59b300c6a5edacespa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordProtozoal DNAspa
dc.subject.keywordProtozoaneng
dc.subject.keywordProtozoal DNAspa
dc.subject.keywordArticlespa
dc.subject.keywordColombiaspa
dc.subject.keywordDiagnostic accuracyspa
dc.subject.keywordDiagnostic test accuracy studyspa
dc.subject.keywordDNA determinationspa
dc.subject.keywordHumanspa
dc.subject.keywordLeishmaniaspa
dc.subject.keywordLimit of detectionspa
dc.subject.keywordLoop mediated isothermal amplificationspa
dc.subject.keywordMicroscopyspa
dc.subject.keywordNonhumanspa
dc.subject.keywordPhlebotominaespa
dc.subject.keywordReal time polymerase chain reactionspa
dc.subject.keywordSensitivity and specificityspa
dc.subject.keywordSkin leishmaniasisspa
dc.subject.keywordSmearspa
dc.subject.keywordAnimalspa
dc.subject.keywordGeneticsspa
dc.subject.keywordIsolation and purificationspa
dc.subject.keywordLeishmaniaspa
dc.subject.keywordNucleic acid amplificationspa
dc.subject.keywordParasitologyspa
dc.subject.keywordProceduresspa
dc.subject.keywordPsychodidaespa
dc.subject.keywordSkin leishmaniasisspa
dc.subject.keywordAnimalsspa
dc.subject.keywordDNAeng
dc.subject.keywordHumansspa
dc.subject.keywordLeishmaniaspa
dc.subject.keywordLeishmaniasiseng
dc.subject.keywordNucleic Acid Amplification Techniquesspa
dc.subject.keywordPsychodidaespa
dc.subject.keywordSensitivity and Specificityspa
dc.titleAnalytical performance of a loop-mediated isothermal amplification assay for leishmania DNA detection in sandflies and direct smears of patients with cutaneous leishmaniasisspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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