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Effect of exercise training on enos expression, NO production and oxygen metabolism in human placenta

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dc.creatorRamírez-Vélez, Robinsonspa
dc.creatorBustamante, Juanitaspa
dc.creatorCzerniczyniec, Analiaspa
dc.creatorAguilar de Plata, Ana C.spa
dc.creatorLores-Arnaiz, Silviaspa
dc.date.accessioned2020-08-19T14:42:47Z
dc.date.available2020-08-19T14:42:47Z
dc.date.created2013-11-14spa
dc.description.abstractAbstract Objective To determine the effects of combined aerobic and resistance exercise training during the second half of pregnancy on endothelial NOS expression (eNOS), nitric oxide (NO) production and oxygen metabolism in human placenta. Methods The study included 20 nulliparous in gestational week 16–20, attending prenatal care at three tertiary hospitals in Colombia who were randomly assigned into one of two groups: The exercise group (n?=?10) took part in an exercise session three times a week for 12 weeks which consisted of: aerobic exercise at an intensity of 55–75% of their maximum heart rate for 60 min and 25 mins. Resistance exercise included 5 exercise groups circuit training (50 repetitions of each) using barbells (1–3 kg/exercise) and low-to-medium resistance bands. The control group (n?=?10) undertook their usual physical activity. Mitochondrial and cytosol fractions were isolated from human placental tissue by differential centrifugation. A spectrophotometric assay was used to measure NO production in cytosolic samples from placental tissue and Western Blot technique to determine eNOS expression. Mitochondrial superoxide levels and hydrogen peroxide were measured to determine oxygen metabolism. Results Combined aerobic and resistance exercise training during pregnancy leads to a 2-fold increase in eNOS expression and 4-fold increase in NO production in placental cytosol (p?=?0.05). Mitochondrial superoxide levels and hydrogen peroxide production rate were decreased by 8% and 37% respectively in the placental mitochondria of exercising women (p?=?0.05). Conclusion Regular exercise training during the second half of pregnancy increases eNOS expression and NO production and decreases reactive oxygen species generation in human placenta. Collectively, these data demonstrate that chronic exercise increases eNOS/NO production, presumably by increasing endothelial shear stress. This adaptation may contribute to the beneficial effects of exercise on the vascular and antioxidant system and in turn reduce the risk of preeclampsia, diabetes or hypertension during pregnancy.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0080225
dc.identifier.issnEISSN: 1932-6203
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/27573
dc.language.isoengspa
dc.publisherPLOS Public Library of Sciencespa
dc.relation.citationIssueNo. 11
dc.relation.citationStartPagee80225
dc.relation.citationTitlePLoS One
dc.relation.citationVolumeVol. 8
dc.relation.ispartofPLoS One, EISSN: 1932-6203, Vol.8, No.11 (November 2013); pp. e80225 spa
dc.relation.urihttps://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0080225&type=printablespa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.sourcePLoS Onespa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.keywordMitochondriaspa
dc.subject.keywordExercisespa
dc.subject.keywordPlacentaspa
dc.subject.keywordHypertensive disorders in pregnancyspa
dc.subject.keywordNitric oxidespa
dc.subject.keywordPregnancyspa
dc.subject.keywordBody weightspa
dc.subject.keywordSuperoxidesspa
dc.titleEffect of exercise training on enos expression, NO production and oxygen metabolism in human placentaspa
dc.title.TranslatedTitleEfecto del entrenamiento físico sobre la expresión de enos, la producción de NO y el metabolismo del oxígeno en la placenta humanaspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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