Ítem
Solo Metadatos

Rv1268c protein peptide inhibiting Mycobacterium tuberculosis H37Rv entry to target cells

Mostrar el registro sencillo de la publicación
dc.creatorOcampo, Marisol
dc.creatorRodríguez, Deisy Carolinaspa
dc.creatorRodríguez, Jorgespa
dc.creatorBermúdez, Maritzaspa
dc.creatorMuñoz, Claudia Marinaspa
dc.creatorPatarroyo, Manuel A.
dc.creatorPatarroyo, Manuel Elkinspa
dc.date.accessioned2020-05-26T00:03:02Z
dc.date.available2020-05-26T00:03:02Z
dc.date.created2013spa
dc.description.abstractTuberculosis (TB) remains one of the most worrying infectious diseases affecting public health around the world; 8.7 million new TB cases were reported in 2011. The search for an Mycobacterium tuberculosis H37Rv protein sequence which is functionally important in host-pathogen interaction has been proposed for developing a new vaccine which will allow efficient and safe control of the spread of this disease. The present study thus reports the results obtained for the Rv1268c protein described in the M. tuberculosis H37Rv genome as a hypothetical unknown, probably secreted, protein based on a highly robust, specific, sensitive and functional approach to the search for potential epitopes to be included in an anti-tuberculosis vaccine. Rv1268c presence was determined by immunoblotting after obtaining polyclonal sera against mycobacterial total sonicate or subcellular fractions. Such sera were used in electron immunomicroscopy (EIM) for confirming protein localisation on the M. tuberculosis envelop by recognising colloidal gold-labelled immunoglobulin. Screening assays revealed the presence of two sequences having high binding activity: one binding A549 alveolar epithelial cells ( 141TGMAALEQYLGSGHAVIVSI160) and other binding U937 monocyte-derived macrophages (21AVALGLASPADAAAGTMYGD40). Such sequences' ability to inhibit mycobacterial entry during in vitro assays was analysed. The structure of synthetic peptides binding to target cells was also determined, bearing in mind the structure-function relationship. These results, together with those obtained for other proteins, have been involved in selecting peptides which might be included in a subunit-based anti-tuberculosis vaccine. © 2013 Elsevier Ltd. All rights reserved.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.bmc.2013.08.018
dc.identifier.issn14643391
dc.identifier.issn09680896
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23554
dc.language.isoengspa
dc.relation.citationEndPage6656
dc.relation.citationIssueNo. 21
dc.relation.citationStartPage6650
dc.relation.citationTitleBioorganic and Medicinal Chemistry
dc.relation.citationVolumeVol. 21
dc.relation.ispartofBioorganic and Medicinal Chemistry, ISSN:14643391, 09680896, Vol.21, No.21 (2013); pp. 6650-6656spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84885187012&doi=10.1016%2fj.bmc.2013.08.018&partnerID=40&md5=70cb371ee109a8fa174ab5b7cc954ef4spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordBacterial proteinspa
dc.subject.keywordtumoreng
dc.subject.keywordSynthetic peptidespa
dc.subject.keywordUnclassified drugspa
dc.subject.keywordAnimal experimentspa
dc.subject.keywordAntibacterial activityspa
dc.subject.keywordArticlespa
dc.subject.keywordBacterial genomespa
dc.subject.keywordBinding affinityspa
dc.subject.keywordCell invasionspa
dc.subject.keywordControlled studyspa
dc.subject.keywordDrug structurespa
dc.subject.keywordDrug synthesisspa
dc.subject.keywordIn vitro studyspa
dc.subject.keywordLung alveolus epitheliumspa
dc.subject.keywordMacrophagespa
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordNonhumanspa
dc.subject.keywordProtein localizationspa
dc.subject.keywordRabbitspa
dc.subject.keywordStructure activity relationspa
dc.subject.keywordTarget cellspa
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordAnti-tuberculosis vaccinespa
dc.subject.keywordEntry inhibitionspa
dc.subject.keywordReceptor-ligand interactionspa
dc.subject.keywordRv1268cspa
dc.subject.keywordSub-cellular localizationspa
dc.subject.keywordSynthetic peptidesspa
dc.subject.keywordTarget cellspa
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordAnimalsspa
dc.subject.keywordBacterial proteinsspa
dc.subject.keywordCell lineeng
dc.subject.keywordCircular dichroismspa
dc.subject.keywordGoldspa
dc.subject.keywordHost-pathogen interactionsspa
dc.subject.keywordHumansspa
dc.subject.keywordImmunoglobulinsspa
dc.subject.keywordMolecular sequence dataspa
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordPeptidesspa
dc.subject.keywordRabbitsspa
dc.subject.keywordRecombinant proteinsspa
dc.subject.keywordAnti-tuberculosis vaccinespa
dc.subject.keywordEntry inhibitionspa
dc.subject.keywordReceptor-ligand interactionspa
dc.subject.keywordRv1268cspa
dc.subject.keywordSub-cellular localizationspa
dc.subject.keywordSynthetic peptidesspa
dc.subject.keywordTarget cellspa
dc.titleRv1268c protein peptide inhibiting Mycobacterium tuberculosis H37Rv entry to target cellsspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
Archivos
Colecciones
Artículos