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New insights into Clostridium Difficile (CD) infection in Latin America : Novel description of toxigenic profiles of diarrhea-associated to CD in Bogotá, Colombia

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dc.contributor.gruplacGrupo de Investigaciones Microbiológicas - UR (GIMUR)spa
dc.creatorRios-Chaparro, Dora-Ines
dc.creatorHerrera, Giovanny
dc.creatorSoto-De Leon, Sara C.
dc.creatorBirchenall, Claudia
dc.creatorPinilla, Darío
dc.creatorPardo Oviedo, Juan Mauricio
dc.creatorJosa, Diego
dc.creatorPatarroyo, Manuel A.
dc.creatorRamírez, Juan David
dc.creator.googleMuñoz, Marinaspa
dc.creator.googleRíos-Chaparro, Dora I.spa
dc.creator.googleHerrera, Giovannyspa
dc.creator.googleSoto-De Leon, Sara C.spa
dc.creator.googleBirchenall, Caudiaspa
dc.creator.googlePinilla, Daríospa
dc.creator.googlePardo-Oviedo, Juan M.spa
dc.creator.googleJosa, Diego F.spa
dc.creator.googlePatarroyo, Manuel A.spa
dc.creator.googleRamírez, Juan D.spa
dc.date.accessioned2020-03-30T22:49:44Z
dc.date.available2020-03-30T22:49:44Z
dc.date.created2018-01-30
dc.date.issued2018
dc.description.abstractClostridium difficile (CD) produces antibiotic associated diarrhea and leads to a broad range of diseases. The source of CD infection (CDI) acquisition and toxigenic profile are factors determining the impact of CD. This study aimed at detecting healthcare facility onset- (HCFO) and community-onset (CO) CDI and describing their toxigenic profiles in Bogotá, Colombia. A total of 217 fecal samples from patients suffering diarrhea were simultaneously submitted to two CDI detection strategies: (i) in vitro culture using selective chromogenic medium (SCM; chromID, bioMérieux), followed verification by colony screening (VCS), and (ii) molecular detection targeting constitutive genes, using two conventional PCR tests (conv. PCR) (conv.16S y conv.gdh) and a quantitative test (qPCR.16s). The CD toxigenic profile identified by any molecular test was described using 6 tests independently for describing PaLoc and CdtLoc organization. High overall CDI frequencies were found by both SCM (52.1%) and conv. PCR (45.6% for conv.16S and 42.4% for conv.gdh), compared to reductions of up to half the frequency by VCS (27.2%) or qPCR.16S (22.6%). Infection frequencies were higher for SCM and conv.16S regarding HCFO but greater for CO concerning conv.gdh, such differences being statistically significant. Heterogeneous toxigenic profiles were found, including amplification with lok1/3 primers simultaneously with other PaLoc markers (tcdA, tcdB or tcdC). These findings correspond the first report regarding the differential detection of CDI using in vitro culture and molecular detection tests in Colombia, the circulation of CD having heterogeneous toxigenic profiles and molecular arrays which could affect the impact of CDI epidemiology. © 2018 Muñoz, Ríos-Chaparro, Herrera, Soto-De Leon, Birchenall, Pinilla, Pardo-Oviedo, Josa, Patarroyo and Ramírez.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.3389/fmicb.2018.00074
dc.identifier.issn1664-302X
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/21306
dc.language.isoengspa
dc.relation.citationTitleFrontiers in Microbiology
dc.relation.citationVolumeVol. 9
dc.relation.ispartofFrontiers in Microbiology, ISSN:1664-302X, Vol. 9 (2018)spa
dc.relation.urihttps://www.frontiersin.org/articles/10.3389/fmicb.2018.00074/fullspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.bibliographicCitationAllegranzi, B., Bagheri Nejad, S., Combescure, C., Graafmans, W., Attar, H., Donaldson, L., Burden of endemic health-care-associated infection in developing countries: systematic review and meta-analysis (2011) Lancet, 377, pp. 228-241spa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subjectClostridium Difficile Toxin Aspa
dc.subjectClostridium Difficile Toxin Bspa
dc.subjectMolecular Markerspa
dc.subjectPrimer Dnaspa
dc.subjectRna 16Sspa
dc.subjectClostridium Difficile Infectionspa
dc.subjectColombiaspa
dc.subjectControlled Studyspa
dc.subjectDiarrheaspa
dc.subjectDisease Controlspa
dc.subjectDna Extractionspa
dc.subjectFeces Analysisspa
dc.subjectGene Expressionspa
dc.subjectGenetic Markerspa
dc.subjectHealth Care Facilityspa
dc.subjectHumanspa
dc.subjectIn Vitro Studyspa
dc.subjectPractice Guidelinespa
dc.subjectReal Time Polymerase Chain Reactionspa
dc.subject.ddcEnfermedadesspa
dc.subject.keywordArticlespa
dc.subject.lembClostridium Difficile Toxin Aspa
dc.subject.lembClostridium Difficile Toxin Bspa
dc.subject.lembMarcador Molecularspa
dc.subject.lembDna imprimaciónspa
dc.subject.lembClostridium difficilespa
dc.titleNew insights into Clostridium Difficile (CD) infection in Latin America : Novel description of toxigenic profiles of diarrhea-associated to CD in Bogotá, Colombiaspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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