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Characterising Mycobacterium tuberculosis Rv1510c protein and determining its sequences that specifically bind to two target cell lines

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dc.creatorVera-Bravo, Ricardospa
dc.creatorTorres, Elizabethspa
dc.creatorValbuena, John J.spa
dc.creatorOcampo, Marisol
dc.creatorRodríguez, Luis E.spa
dc.creatorPuentes, Álvarospa
dc.creatorGarcía, Javier E.spa
dc.creatorCurtidor, Hernandospa
dc.creatorCortés, Jimenaspa
dc.creatorVanegas, Magnoliaspa
dc.creatorRivera, Zuly J.spa
dc.creatorDíaz, Andreaspa
dc.creatorCalderon, Martha N.spa
dc.creatorPatarroyo, Manuel A.spa
dc.creatorPatarroyo, Manuel E.spa
dc.date.accessioned2020-08-06T16:20:27Z
dc.date.available2020-08-06T16:20:27Z
dc.date.created2005-07-08spa
dc.description.abstractThe process of Mycobacterium tuberculosis infection of the macrophage implies a very little-known initial recognition and adherence step, important for mycobacterial survival; many proteins even remain like hypothetical. The Rv1510c gene, encoding a putatively conserved membrane protein, was investigated by analysing the M. tuberculosis genome sequence data reported by Cole et al. and a previous report that used PCR assays to show that the Rv1510 gene was only present in M. tuberculosis. This article confirmed all the above and identified the transcribed gene in M. tuberculosis, Mycobacterium africanum, and in M. tuberculosis clinical isolates. Antibodies raised against peptides from this protein recognised a 44 kDa band, corresponding to Rv1510c theoretical mass (44,294 Da). Assays involving synthetic peptides covering the whole protein binding to U937 and A549 cell lines led to recognising five high activity binding peptides in the Rv1510 protein: 11094, 11095, 11105, 11108, and 11111. Their affinity constants and Hill coefficients were determined by using U937 cells. Cross-linking assays performed with some of these HABPs showed that they specifically bound to a U937 cell line 51 kDa protein, but not to Hep G2 or red blood cell proteins, showing this interaction’s specificity.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.bbrc.2005.05.018
dc.identifier.issnISSN: 0006-291X
dc.identifier.issnEISSN: 1090-2104
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/26011
dc.language.isoengspa
dc.publisherElsevierspa
dc.relation.citationEndPage781
dc.relation.citationIssueNo. 3
dc.relation.citationStartPage771
dc.relation.citationTitleBiochemical and Biophysical Research Communications
dc.relation.citationVolumeVol. 332
dc.relation.ispartofBiochemical and Biophysical Research Communications, ISSN: 0006-291X;EISSN: 1090-2104, Vol.332, No.3 (2005); pp.771-781spa
dc.relation.urihttps://www.sciencedirect.com/science/article/abs/pii/S0006291X05009642spa
dc.rights.accesRightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.accesoRestringido (Acceso a grupos específicos)spa
dc.sourceBiochemical and Biophysical Research Communicationsspa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.keywordRv1510spa
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordTuberculosisspa
dc.subject.keywordPeptidesspa
dc.subject.keywordBindingspa
dc.titleCharacterising Mycobacterium tuberculosis Rv1510c protein and determining its sequences that specifically bind to two target cell linesspa
dc.title.TranslatedTitleCaracterizando la proteína Mycobacterium tuberculosis Rv1510c y determinando sus secuencias que se unen específicamente a dos líneas celulares dianaspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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