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Use of single-nucleotide polymorphisms (SNPs) to distinguish gene expression subtypes of chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME)

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dc.creatorShimosako, Nanaspa
dc.creatorKerr, Jonathan Rspa
dc.date.accessioned2020-05-25T23:56:03Z
dc.date.available2020-05-25T23:56:03Z
dc.date.created2014spa
dc.description.abstractMethods To identify SNP allele associations with CFS/ME and CFS/ME subtypes, we tested genomic DNA of patients with CFS/ME (n=108), patients with endogenous depression (n=17) and normal blood donors (n=68) for 504 human SNP alleles located within 88 CFS-associated human genes using the SNP Genotyping GoldenGate Assay (Illumina, San Diego, California, USA). 360 ancestry informative markers (AIM) were also examined.Aims We have reported gene expression changes in patients with chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME) and the fact that such gene expression data can be used to identify subtypes of CFS/ME with distinct clinical phenotypes. Due to the difficulties in using a comparative gene expression method as an aid to CFS/ME disease and subtype-specific diagnosis, we have attempted to develop such a method based on single-nucleotide polymorphism (SNP) analysis.Conclusions This study provides evidence that human SNPs located within CFS/ME associated genes are associated with particular genomic subtypes of CFS/ME. Further work is required to develop this into a clinically useful subtype-specific diagnostic test.Results 21 SNPs were significantly associated with CFS/ME compared with depression and normal groups. 148 SNP alleles had a significant association with one or more CFS/ME subtypes. For each subtype, associated SNPs tended to be grouped together within particular genes. AIM SNPs indicated that 4 subjects were of Asian origin while the remainder were Caucasian. Hierarchical clustering of AIM data revealed the relatedness between 2 couples of patients with CFS only and confirmed the overall heterogeneity of all subjects.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1136/jclinpath-2014-202597
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22308
dc.language.isoengspa
dc.publisherBMJ Publishing Groupspa
dc.relation.citationEndPage1083
dc.relation.citationIssueNo. 12
dc.relation.citationStartPage1078
dc.relation.citationTitleJournal of Clinical Pathology
dc.relation.citationVolumeVol. 67
dc.relation.ispartofJournal of Clinical Pathology, Vol.67, No.12 (2014); pp. 1078-1083spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84910138360&doi=10.1136%2fjclinpath-2014-202597&partnerID=40&md5=6a498d197e6fb737ad0ce00d34d37d32spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordGenomic DNAspa
dc.subject.keywordChroniceng
dc.subject.keywordTranscriptomespa
dc.subject.keywordAdultspa
dc.subject.keywordAllelespa
dc.subject.keywordArticlespa
dc.subject.keywordAsianspa
dc.subject.keywordBinding sitespa
dc.subject.keywordBlood donorspa
dc.subject.keywordCaucasianspa
dc.subject.keywordChronic fatigue syndromespa
dc.subject.keywordControlled studyspa
dc.subject.keywordDiagnostic testspa
dc.subject.keywordEndogenous depressionspa
dc.subject.keywordFemalespa
dc.subject.keywordGene expressionspa
dc.subject.keywordGenetic associationspa
dc.subject.keywordGenotypespa
dc.subject.keywordHumanspa
dc.subject.keywordMajor clinical studyspa
dc.subject.keywordMalespa
dc.subject.keywordPhenotypespa
dc.subject.keywordSequence analysisspa
dc.subject.keywordSingle nucleotide polymorphismspa
dc.subject.keywordChronic fatigue syndromespa
dc.subject.keywordClassificationspa
dc.subject.keywordCluster analysisspa
dc.subject.keywordDepressionspa
dc.subject.keywordGenetic predispositionspa
dc.subject.keywordGeneticsspa
dc.subject.keywordMiddle agedspa
dc.subject.keywordSingle nucleotide polymorphismspa
dc.subject.keywordAdultspa
dc.subject.keywordCluster Analysisspa
dc.subject.keywordDepressionspa
dc.subject.keywordFatigue Syndromeeng
dc.subject.keywordFemalespa
dc.subject.keywordGenetic Predisposition to Diseasespa
dc.subject.keywordGenotypespa
dc.subject.keywordHumansspa
dc.subject.keywordMalespa
dc.subject.keywordMiddle Agedspa
dc.subject.keywordPolymorphismeng
dc.subject.keywordTranscriptomespa
dc.titleUse of single-nucleotide polymorphisms (SNPs) to distinguish gene expression subtypes of chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME)spa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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