Ítem
Acceso Abierto
Uso de la matriz extracelular como material para ingeniería de tejidos
| dc.contributor.advisor | Briceňo, Juan Carlos | |
| dc.creator | Araque, Juan | |
| dc.creator | Sanchez, Diana | |
| dc.creator | Navarro, Javier | |
| dc.creator | Briceňo, Juan | |
| dc.creator.degree | Especialista en Cirugía Cardiovascular | |
| dc.date.accessioned | 2014-07-18T14:31:10Z | |
| dc.date.available | 2014-07-18T14:31:10Z | |
| dc.date.created | 2014-07-07 | |
| dc.date.issued | 2014 | |
| dc.description | Introducción: La evaluación de injertos vasculares de submucosa de intestino delgado para la regeneración de vasos sanguíneos ha producido una permeabilidad variable (0-100%) que ha sido concurrente con la variabilidad en las técnicas de fabricación. Metodología: Investigamos los efectos de fabricación en permeabilidad y regeneración en un diseño experimental de 22factorial que combino: 1) preservación (P) o remoción (R) de la capa estratum compactum del intestino, y 2) deshidratada (D) o hidratada (H), dentro de cuatro grupos de estudio (PD, RD, PH, RH). Los injertos fueron implantados en las Arterias Carótidas de porcinos (ID 4.5mm, N=4, 7d). Permeabilidad, trombogenicidad, reacción inflamatoria, vascularización, infiltración de fibroblastos, perfil de polarización de macrófagos y fuerza tensil biaxial fueron evaluadas. Resultados: Todos los injertos PD permanecieron permeables (4/4), pero tuvieron escasa vascularización e infiltración de fibroblastos. El grupo RD permaneció permeable (4/4), presentó una extensa vascularización e infiltración de fibroblastos, y el mayor número del fenotipo de macrófagos (M2) asociado a regeneración. El grupo RH presentó menor permeabilidad (3/4), una extensa vascularización e infiltración de fibroblastos, y un perfil dominante de M2. El grupo PH presentó el menor grado de permeabilidad, y a pesar de mayor infiltración celular que PD, exhibió un fenotipo de macrófagos dominante adverso. La elasticidad de los injertos R evolucionó de una manera similar a las Carótidas nativas (particularmente RD, mientras que los injertos P mantuvieron su rigidez inicial. Discusión: Concluimos que los parámetros de fabricación afectan drásticamente los resultados, siendo los injertos RD los que arrojaron mejores resultados. | spa |
| dc.description.abstract | Introduction: Evaluation of small intestine submucosa vascular grafts for the regeneration of blood vessels has produced variable patency (0-100%) that has been concurrent with variability in fabrication techniques. Methodology: We herein investigated the effects of fabrication on patency and regeneration in a 22 factorial experimental design that combined: 1) preservation (P) or removal (R) of the stratum compactum layer of the intestine and 2) dehydrated (D) or hydrated (H) state, into four study groups (PD, RD, PH, RH). Grafts were implanted in the carotid arteries of swine (ID 4.5 mm, n=4, 7d). Patency, thrombogenicity, inflammatory reaction, vascularization, fibroblast infiltration, macrophage polarization profile and biaxial tensile mechanics were assessed. Results: All PD grafts remained patent (4/4) but had scarce vascularization and fibroblast infiltration. RD had sustained patency (4/4), extensive vascularization and fibroblast infiltration and the highest number of regeneration-associated phenotype macrophages (M2). RH had lower patency (3/4), extensive vascularization and fibroblast infiltration, an M2 dominant profile but less M2 macrophages compared to RD. PH had the lowest patency (2/4), and in spite of higher cellular infiltration than PD, they exhibited a dominant adverse macrophage phenotype. Elasticity of R grafts evolved towards that of native carotids (particularly RD), while P grafts kept their initial stiffness. Discussion: We concluded that fabrication parameters drastically affected the outcome criteria, with the best result produced by RD grafts. | eng |
| dc.description.sponsorship | ninguno | spa |
| dc.format.mimetype | application/pdf | |
| dc.identifier.doi | https://doi.org/10.48713/10336_8671 | |
| dc.identifier.uri | http://repository.urosario.edu.co/handle/10336/8671 | |
| dc.language.iso | spa | |
| dc.publisher | Universidad del Rosario | spa |
| dc.publisher.department | Facultad de Medicina | spa |
| dc.publisher.program | Especialización en Cirugía Cardiovascular | spa |
| dc.rights.accesRights | info:eu-repo/semantics/openAccess | |
| dc.rights.acceso | Abierto (Texto Completo) | spa |
| dc.rights.cc | Atribución-NoComercial-SinDerivadas 2.5 Colombia | spa |
| dc.rights.licencia | EL AUTOR, manifiesta que la obra objeto de la presente autorización es original y la realizó sin violar o usurpar derechos de autor de terceros, por lo tanto la obra es de exclusiva autoría y tiene la titularidad sobre la misma. PARGRAFO: En caso de presentarse cualquier reclamación o acción por parte de un tercero en cuanto a los derechos de autor sobre la obra en cuestión, EL AUTOR, asumirá toda la responsabilidad, y saldrá en defensa de los derechos aquí autorizados; para todos los efectos la universidad actúa como un tercero de buena fe. EL AUTOR, autoriza a LA UNIVERSIDAD DEL ROSARIO, para que en los términos establecidos en la Ley 23 de 1982, Ley 44 de 1993, Decisión andina 351 de 1993, Decreto 460 de 1995 y demás normas generales sobre la materia, utilice y use la obra objeto de la presente autorización. -------------------------------------- POLITICA DE TRATAMIENTO DE DATOS PERSONALES. Declaro que autorizo previa y de forma informada el tratamiento de mis datos personales por parte de LA UNIVERSIDAD DEL ROSARIO para fines académicos y en aplicación de convenios con terceros o servicios conexos con actividades propias de la academia, con estricto cumplimiento de los principios de ley. Para el correcto ejercicio de mi derecho de habeas data cuento con la cuenta de correo habeasdata@urosario.edu.co, donde previa identificación podré solicitar la consulta, corrección y supresión de mis datos. | spa |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/2.5/co/ | |
| dc.source.bibliographicCitation | 1. DANE, Defunciones por grupo de edad y sexo, según departamentos de ocurrencia y grupos de causas de defunción, Tabla 11, 2009. | |
| dc.source.bibliographicCitation | 2. Who, World Health Organization, WHO: Ten leading causes of deaths in high-income and low-income and middle-income countries. 2008. | |
| dc.source.bibliographicCitation | 3. Moll FL, Powell JT, Fraederich G, Verzini F, Haulon S, Waltham M, et al. Management of Abdominal Aortic Aneurysma Clinical Practice Guidelines of the European Society for Vascular Surgery. Eur J Vasc Endovasc Surg. 2011; Vol (41):51-58.. | |
| dc.source.bibliographicCitation | 4. Baas AF, Kranendonk SE. Abdominal Aortic Aneurism. Clinical Cardiogenetics. 2011; Cap 27. | |
| dc.source.bibliographicCitation | 5. Goeau-Brissonniere O, Javerliat I, Coggia M. Advances in Vascular Grafts for Thoraco-Abdominal Aortic Open Surgery. Thoraco-Abdominal Aorta: Surgical and Anesthetic Management. 2011; (54). | |
| dc.source.bibliographicCitation | 6. Piterina AV, Cloonan AJ, Meaney CL, Davis LM, Callanan A, Walsh M T, Mcgloughlin TM. ECM-based materials in cardiovascular applications: inherent healing potential and augmentation of native regenerative processes, Int J Mol Sci. 2009; (10): 4375–4417. | |
| dc.source.bibliographicCitation | 7. Fauci AS, Braunwald E, Kasper DL, Hauser SL, Longo DL, Jameson JL, et al. Harrison’s Principles of Internal Medicine. 17th ed. 2008. New York: McGraw Hill. | |
| dc.source.bibliographicCitation | 8. Bittl J. Catheter Interventions for Hemodialysis Fistulas and Grafts. J Am Coll Cardiol Intv. 2010; (3)1–11. | |
| dc.source.bibliographicCitation | 9. Chalem F. et al. tratado de medicina interna, 4ª ed. 2005; editorial médica celsus. (1):1159. | |
| dc.source.bibliographicCitation | 10. Ethier J, Bragg-Gresham JL, Piera L, et al. aspirin prescription and outcomes in hemodialysis patients: the dialysis outcomes and practice patterns study (dopps). am j kidney dis. 2007; 50(4):602-11. | |
| dc.source.bibliographicCitation | 11. Berardinelli L, grafts and graft materials as vascular substitutes for haemodialysis access construction. eur j vasc endovasc surg. 2006; (32): 203–211. | |
| dc.source.bibliographicCitation | 12. Scott E, Glickman M, conduits for hemodialysis access. semin vasc surg, 2007; (20): 158-163. | |
| dc.source.bibliographicCitation | 13. Jiménez-Almonacid P, Del Río P, Lasala M, et al. primer acceso vascular no autólogo para hemodiálisis. Nefrología.2004; (24):559-563. | |
| dc.source.bibliographicCitation | 14. Stehman-Breen K, Sherrard D, Gillen D, Caps M, determinants of type and timing of initial permanent hemodialysis vascular access. kidney international. 2000; (57):639–645. | |
| dc.source.bibliographicCitation | 15. Roy-Chaudhery P, Kelly B, Melhem M, et al. vascular access in hemodialysis: issues, management, and emerging concepts. cardiol clin. 2005; (23):249–273. | |
| dc.source.bibliographicCitation | 16. Naito Y, Shinoka T, Duncan, D, Hibino N, Solomon D, Cleary M, Rathore A, Fein C, Church S, Breuer C. vascular tissue engineering: towards the next generation vascular grafts. adv drug deliver rev.2011; (63): 312 – 323 | |
| dc.source.bibliographicCitation | 17. Mcallister et al. hemodialysis access with an autologous tissue-engineered vascular graft. Lancet. 2009; (373)1440–1446. | |
| dc.source.bibliographicCitation | 18. Kaushal S, Amiel G E, Guleserian K J, Shapira O M, Perry T, Sutherland F W, Rabkin E, Moran AM, Schoen FJ, Atala A, Soker S, Bischo J, Mayer JE. functional small-diameter neovessels created using endothelial progenitor cells expanded ex vivo. nat med.2001; (7): 1035–1040. | |
| dc.source.bibliographicCitation | 19. Soletti L, Hong Y, Guan J, Stankus J, El-Kurdi M S, Wagner W R, Vorp D A. a bilayered elastomeric scaffold for tissue engineering of small diameter vascular grafts. acta biomater 2010; (6):110 – 122. | |
| dc.source.bibliographicCitation | 20. Ratcliffe A, tissue engineering of vascular grafts. matrix boil. 2000; (19):353–357. | |
| dc.source.bibliographicCitation | 21. Sandusky GE, Lantz GC, Badylak SF. healing comparison of small intestine submucosa and eptfe grafts in the canine carotid artery. j surg res. 1995; (58):415–420. | |
| dc.source.bibliographicCitation | 22. Badylak SF, Freytes DO, Gilbert TW. extracellular matrix as a biological scaffold material: structure and function. acta biomater. 2009; (5):1 – 13. | |
| dc.source.bibliographicCitation | 23. Kim BS, Park I K, Hoshiba T, Tiang HL, Choi YJ, Akaike T, Cho CS. design of artificial extracellular matrices for tissue engineering. prog polym sci. 2011; (36): 238– 268. | |
| dc.source.bibliographicCitation | 24. Badylak SF, Coffey AC, Geddes LE, Lantz GC. tissue graft composition. us patent. 1988; (4):178. | |
| dc.source.bibliographicCitation | 25. Lantz GC, Badylak SF, Hiles MC, Coffey AC, Geddes LE, Kokini K, Sandusky GE, Morff RJ. small intestinal submucosa as a vascular graft: a review. j invest surg.1993; (6):297–310. | |
| dc.source.bibliographicCitation | 26. Badylak SF, Gilbert T, Myers-Irvin J. the extracellular matrix as a biologic scaffold for tissue engineering. in tissue engineering. 2008: 121-128. | |
| dc.source.bibliographicCitation | 27. Badylak SF. xenogeneic extracellular matrix as a scaffold for tissue reconstruction. transpl immunol. 2004; (12):367 – 377. | |
| dc.source.bibliographicCitation | 28. Lantz GC, Badylak SF, Coey AC, Geddes IE, Blevins WE. small intestinal submucosa as a small-diameter arterial graft in the dog. j invest surg. 1990; (3):217–227. | |
| dc.source.bibliographicCitation | 29. Sandusky GE, Badylak SF, Morff RJ, Johnson WD, Lantz G. histologic findings after in vivo placement of small intestine submucosal vascular grafts and saphenous vein grafts in the carotid artery in dogs. am j pathol.1992; (140): 317–324. | |
| dc.source.bibliographicCitation | 30. Huynh T, Abraham G, Murray J, Brockbank K, Hagen PO, Sullivan S. remodeling of an acellular collagen graft into a physiologically responsive neovessel. nat biotechnol. 1999; (17):1083–1086. | |
| dc.source.bibliographicCitation | 31. Kohler TR, Kirkman TR, Kraiss IW, Zierler BK, Clowes AW. increased blood flow inhibits neointimal hyperplasia in endothelialized vascular grafts. circ res.1991; (69):1557–1565. | |
| dc.source.bibliographicCitation | 32. Holzapfel GA, Thomas C, Ogden RW. a new constitutive framework for arterial wall mechanics and a comparative study of material models. printed in cardiovascular soft tissue mechanics. 2004. | |
| dc.source.bibliographicCitation | 33. Zulliger MA, Fridez P, Hayashi K, Stergiopulos N. a strain energy function for arteries accounting for wall composition and structure. journal of biomechanics. 2004;(37), 989-1000. | |
| dc.source.bibliographicCitation | 34. Gundiah N, Ratcliffe MB, Pruitt IA. determination of strain energy function for arterial elastin: experiments using histology and mechanical tests. journal of biomechanics. 2007; (40):586-594. | |
| dc.source.bibliographicCitation | 35. Rezakhaniha R, Stergiopulos N. a structural model of the venous wall considering elastin anisotropy. journal of biomechanical engineering.2014;(37). | |
| dc.source.bibliographicCitation | 36. Lanir Y. a structural theory for the homogenous biaxial stress-strain relationships in flat collagenous tissues. j. biomechanics. 1979; (12):423-436. | |
| dc.source.bibliographicCitation | 37. Fung YCh. biomechanics: mechanical properties of living tissues. second edition, springer-verlag, 1993. | |
| dc.source.bibliographicCitation | 38. Sacks MS. biaxial mechanical evaluation of planar biological materials. journal of elasticity. 2000; (61):199-246. | |
| dc.source.bibliographicCitation | 39. Choi HS, Vito RP. two dimensional stress strain relationship for canine pericardium. journal of biomedical engineering.1990;(112):153-159. | |
| dc.source.bibliographicCitation | 40. Aristizábal HA. caracterización mecánica de injertos de submucosa intestinal porcina (sis) en aplicaciones vasculares, proyecto de grado (pregrado), universidad de los andes. Bogotá. 2009. | |
| dc.source.bibliographicCitation | 41. Beltrán R. estudio de la remodelación de la pared arterial usando soportes de sis, proyecto de grado, universidad de los andes, bogotá 2007. | |
| dc.source.bibliographicCitation | 42. Navarro J. caracterización mecánica de injertos de submucosa intestinal y arterias, por medio de ensayos de tensión biaxiales y uniaxiales. proyecto intermedio. universidad de los andes. Bogotá. 2009. | |
| dc.source.bibliographicCitation | 43. Sánchez, D. análisis mecánico de injertos de colágeno en un modelo in vivo, proyecto de grado, universidad de los andes. Bogotá. 2005. | |
| dc.source.bibliographicCitation | 44. Castañeda N. cálculo de esfuerzos y deformaciones en la pared arterial. proyecto de grado, universidad de los andes. Bogotá. 2009. | |
| dc.source.bibliographicCitation | 45. Navarro J. caracterización mecánica de injertos de colágenos implantados en vena yugular, por medio de ensayos de tensión biaxial y de presión-volumen. proyecto de grado. universidad de los andes. Bogotá. 2010. | |
| dc.source.bibliographicCitation | 46. Lally C, Reid A J, Prendergast PJ. elastic behavior of porcine coronary artery tissue under uniaxial and equibiaxial tension. annals of biomedical engineering. 2004; (32): 1355-1364. | |
| dc.source.bibliographicCitation | 47. Sacks MS. a method for planar biaxial mechanical testing that includes in-plan shear. journal of biomechanical engineering. 1999; (121): 551-555. Dee KC., mechanical characterization of collagen fibers and scaffolds for tissue engineering. Biomaterials. 2003; (24):3805-3813. | |
| dc.source.bibliographicCitation | 48. Vesely I. the role of elastin in aortic valve mechanics. journal of biomechanics. 1998; (31):115-123. | |
| dc.source.bibliographicCitation | 49. Dinnar U. cardiovascular fluid dynamics. crc press, united states, florida, 1981. | |
| dc.source.bibliographicCitation | 50. Brüel A, Oxlund H. changes in biomechanical properties, composition of collagen and elastin, and advanced glycation endproducts of the rat aorta in relation to age. Atherosclerosis.1996;(127): 155-65. | |
| dc.source.bibliographicCitation | 51. Lee T C, Midura RJ, Hascall VC, Vesely I. the effect of elastin damage on the mechanics of the aortic valve. journal of biomechanics.2001; (34):203-210. | |
| dc.source.bibliographicCitation | 52. Chesler NC, Thompson-Figueroa J, Millburne K. measurements of mouse pulmonary artery biomechanics. journal of biomechanical engineering. 2004; (126):309-314. | |
| dc.source.bibliographicCitation | 53. Meyerson SI , Moawad J, Loth F, Skelly Cl, Bassiouny HS, Mckinsey JF, Gewertz Bl, Schwartz lB. effective hemodynamic diameter: an intrinsic property of vein grafts with predictive value for patency. j vasc surg. 2000;(31): 910 – 917. | |
| dc.source.bibliographicCitation | 54. Wei HJ, Liang HC, Lee MH, Huang YC, Chang Y, Sung HW. construction of varying porous structures in acellular bovine pericardia as a tissue-engineering extracellular matrix. Biomaterials. 2005; (26):1905 – 1913. | |
| dc.source.bibliographicCitation | 55. Zilla P, Bezuidenhout D, Human P. prosthetic vascular grafts: wrong models, wrong questions and no healing. Biomaterials.2007;(28):5009 – 5027. | |
| dc.source.bibliographicCitation | 56. Ratner BD, Hoffman AS, Schoen FJ, Lemons JE. biomaterials science: an introduction to materials in medicine. academic press. 2004. elsevier academic press, second ed. | |
| dc.source.bibliographicCitation | 57. D’amore A, Stella JA, Wagner WR, Sacks MS. characterization of the complete fiber network topology of planar fibrous tissues and scaffolds. Biomaterials. 2010(31): 5345 – 5354 | |
| dc.source.bibliographicCitation | 58. Voronov R, Yangordon S, Sikavitsas VJ, Papavassiliou DV. computational modeling of flow-induced shear stresses within 3d salt-leached porous scaffolds imaged via micro-ct. journal of biomechanics; (43): 1279 – 1286. | |
| dc.source.bibliographicCitation | 59. Hibbeler, R. C., structural analysis. prentice hall. 2008. | |
| dc.source.instname | instname:Universidad del Rosario | spa |
| dc.source.reponame | reponame:Repositorio Institucional EdocUR | spa |
| dc.subject | SIS (submucosa de intestino delgado) | spa |
| dc.subject | Macrófago | spa |
| dc.subject | Injerto vascular | spa |
| dc.subject | Biomecánica de tejido blando | spa |
| dc.subject | ingeniería de tejido arterial. | spa |
| dc.subject.ddc | Ciencias médicas, Medicina | |
| dc.subject.decs | Injerto vascular | spa |
| dc.subject.decs | Biomecánica de tejido blando | spa |
| dc.subject.decs | Ingeniería de tejido arterial | spa |
| dc.subject.keyword | SIS (small intestine submucosa) | eng |
| dc.subject.keyword | macrophage | eng |
| dc.subject.keyword | vascular grafts | eng |
| dc.subject.keyword | scaffold | eng |
| dc.subject.keyword | soft tissue biomechanics | eng |
| dc.subject.keyword | arterial tissue engineering | eng |
| dc.subject.lemb | Genética | spa |
| dc.subject.lemb | Biología celular | spa |
| dc.title | Uso de la matriz extracelular como material para ingeniería de tejidos | spa |
| dc.type | masterThesis | eng |
| dc.type.hasVersion | info:eu-repo/semantics/submittedVersion | |
| dc.type.spa | Trabajo de grado | spa |
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