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The role of Mycobacterium tuberculosis Rv3166c protein-derived high-activity binding peptides in inhibiting invasion of human cell lines

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dc.creatorOcampo, Marisol
dc.creatorAristizábal-Ramírez, Danielspa
dc.creatorRodríguez, Diana M.spa
dc.creatorMuñoz, Marinaspa
dc.creatorCurtidor, Hernandospa
dc.creatorVanegas, Magnoliaspa
dc.creatorPatarroyo, Manuel A.spa
dc.creatorPatarroyo, Manuel E.spa
dc.date.accessioned2020-05-25T23:58:35Z
dc.date.available2020-05-25T23:58:35Z
dc.date.created2012spa
dc.description.abstractGiven the urgent need for designing a new antituberculosis vaccine conferring total protection on patients of all ages, following the line of research adopted by our institute, this work has identified Mycobacterium tuberculosis (Mtb) Rv3166c protein high-activity binding peptides (HABPs) which are able to inhibit bacterial invasion of U937 (monocyte-derived macrophages) and A549 (type II alveolar epithelial cells) cell lines. The presence and transcription of the rv3166c gene in the Mtb species complex was confirmed by polymerase chain reaction (PCR) and reverse transcriptase-PCR; Rv3166c expression was evaluated by western blot and cellular localisation confirmed by immunoelectron microscopy. Its presence was mainly determined on cell surface. Sixteen peptides covering its entire length were chemically synthesised and tested for their ability to bind to U937 and A549 cells. Two U937 HABPs were identified and three for A549, one of them being shared by both cell lines. The four HABPs found inhibited Mtb entry by 15.0794.06. These results led us to including Rv3166c HABPs as candidates for further studies contributing towards the search for a multiepitope, chemically synthesised, subunit-based antituberculosis vaccine. © 2012 The Author.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1093/protein/gzs011
dc.identifier.issn17410126
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22890
dc.language.isoengspa
dc.relation.citationEndPage242
dc.relation.citationIssueNo. 5
dc.relation.citationStartPage235
dc.relation.citationTitleProtein Engineering, Design and Selection
dc.relation.citationVolumeVol. 25
dc.relation.ispartofProtein Engineering, Design and Selection, ISSN:17410126, Vol.25, No.5 (2012); pp. 235-242spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84860495384&doi=10.1093%2fprotein%2fgzs011&partnerID=40&md5=28960f8d319fb37c7179c98a0445a5d3spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordA549 cellsspa
dc.subject.keywordImmunoelectroneng
dc.subject.keywordBacterial invasionspa
dc.subject.keywordBinding peptidespa
dc.subject.keywordCell linesspa
dc.subject.keywordCell surfacesspa
dc.subject.keywordHuman cell linesspa
dc.subject.keywordImmunoelectron microscopyspa
dc.subject.keywordLocalisationspa
dc.subject.keywordMonocyte-derived macrophagesspa
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordSynthetic peptidespa
dc.subject.keywordType IIspa
dc.subject.keywordWestern blotsspa
dc.subject.keywordCell membranesspa
dc.subject.keywordGenesspa
dc.subject.keywordPeptidesspa
dc.subject.keywordPolymerase chain reactionspa
dc.subject.keywordTranscriptionspa
dc.subject.keywordTubes (components)spa
dc.subject.keywordVaccinesspa
dc.subject.keywordCell culturespa
dc.subject.keywordBacterial proteinspa
dc.subject.keywordCarrier proteinspa
dc.subject.keywordHigh activity binding peptidespa
dc.subject.keywordProtein rv 3166cspa
dc.subject.keywordUnclassified drugspa
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordArticlespa
dc.subject.keywordCell invasionspa
dc.subject.keywordCell linespa
dc.subject.keywordCell strain U937spa
dc.subject.keywordCell surfacespa
dc.subject.keywordCellular distributionspa
dc.subject.keywordControlled studyspa
dc.subject.keywordEpithelium cellspa
dc.subject.keywordGene amplificationspa
dc.subject.keywordGenetic transcriptionspa
dc.subject.keywordImmunoelectron microscopyspa
dc.subject.keywordLung alveolus epitheliumspa
dc.subject.keywordMacrophagespa
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordNonhumanspa
dc.subject.keywordPriority journalspa
dc.subject.keywordProtein analysisspa
dc.subject.keywordProtein bindingspa
dc.subject.keywordProtein synthesisspa
dc.subject.keywordReverse transcription polymerase chain reactionspa
dc.subject.keywordWestern blottingspa
dc.subject.keywordAnimalsspa
dc.subject.keywordAntibodieseng
dc.subject.keywordBacterial Proteinsspa
dc.subject.keywordHumansspa
dc.subject.keywordMacrophagesspa
dc.subject.keywordMembrane Proteinsspa
dc.subject.keywordMicroscopyeng
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordPulmonary Alveolispa
dc.subject.keywordRabbitsspa
dc.subject.keywordTuberculosis Vaccinesspa
dc.subject.keywordU937 Cellsspa
dc.subject.keywordBacteria (microorganisms)spa
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordAntituberculosis vaccinespa
dc.subject.keywordHuman cell linespa
dc.subject.keywordInvasion inhibitionspa
dc.subject.keywordSub-cellular localisationspa
dc.subject.keywordSynthetic peptidespa
dc.titleThe role of Mycobacterium tuberculosis Rv3166c protein-derived high-activity binding peptides in inhibiting invasion of human cell linesspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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