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Proteolytic activity regarding Sarconesiopsis magellanica (Diptera: Calliphoridae) larval excretions and secretions

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dc.creatorPinilla, Yudi T.spa
dc.creatorMoreno-Pérez, Darwin A.spa
dc.creatorPatarroyo, Manuel A.spa
dc.creatorBello, Felio J.spa
dc.date.accessioned2020-05-26T00:03:23Z
dc.date.available2020-05-26T00:03:23Z
dc.date.created2013spa
dc.description.abstractSarconesiopsis magellanica (Diptera: Calliphoridae) is a medically important necrophagous fly which is used for establishing the post-mortem interval. Diptera maggots release proteolytic enzymes contained in larval excretion and secretion (ES) products playing a key role in digestion. Special interest in proteolytic enzymes has also been aroused regarding understanding their role in wound healing since they degrade necrotic tissue during larval therapy. This study was thus aimed at identifying and characterising S. magellanica proteolytic enzyme ES products for the first time. These products were obtained from first-, second- and third-instar larvae taken from a previously-established colony. ES proteins were separated by SDS-PAGE and their proteolytic activity was characterised by zymograms and inhibition assays involving BAPNA (N?-benzoyl- dl-Arg- p-nitroanilide) and SAPNA substrates, using synthetic inhibitors. The protein profile ranged from ~69. kDa to ~23. kDa; several of them coincided with the Lucilia sericata ES protein profile. Serine-protease hydrolysis activity (measured by zymogram) was confirmed when a ~25. kDa band disappeared upon ES incubation with PMSF inhibitor at pH 7.8. Analysis of larval ES proteolytic activity on BAPNA and SAPNA substrates (determined by using TLCK and TPCK specific inhibitors) suggested a greater amount of trypsin-like protease. These results support the need for further experiments aimed at validating S. magellanica use in larval therapy. © 2013 Elsevier B.V.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.actatropica.2013.09.020
dc.identifier.issn0001706X
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23586
dc.language.isoengspa
dc.relation.citationEndPage691
dc.relation.citationIssueNo. 3
dc.relation.citationStartPage686
dc.relation.citationTitleActa Tropica
dc.relation.citationVolumeVol. 128
dc.relation.ispartofActa Tropica, ISSN:0001706X, Vol.128, No.3 (2013); pp. 686-691spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84892519619&doi=10.1016%2fj.actatropica.2013.09.020&partnerID=40&md5=ced021acd4a9c524b1a2e986ce0e4670spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordSerine proteinasespa
dc.subject.keywordEnzyme activityspa
dc.subject.keywordExcretionspa
dc.subject.keywordFlyspa
dc.subject.keywordLarvaspa
dc.subject.keywordSecretionspa
dc.subject.keywordArticlespa
dc.subject.keywordControlled studyspa
dc.subject.keywordDipteraspa
dc.subject.keywordEnzyme inhibition assayspa
dc.subject.keywordEnzyme substratespa
dc.subject.keywordHydrolysisspa
dc.subject.keywordLarvaspa
dc.subject.keywordNonhumanspa
dc.subject.keywordPolyacrylamide gel electrophoresisspa
dc.subject.keywordProtein degradationspa
dc.subject.keywordProtein determinationspa
dc.subject.keywordProtein secretionspa
dc.subject.keywordSarconesiopsis magellanicaspa
dc.subject.keywordSpecies differencespa
dc.subject.keywordZymographyspa
dc.subject.keywordCalliphoridaespa
dc.subject.keywordDipteraspa
dc.subject.keywordLucilia sericataspa
dc.subject.keywordLarvaespa
dc.subject.keywordLarval therapyspa
dc.subject.keywordNecrotic woundsspa
dc.subject.keywordProteasesspa
dc.subject.keywordProteolytic activityspa
dc.subject.keywordSspa
dc.subject.keywordMagellanicaspa
dc.subject.keywordAnimalsspa
dc.subject.keywordBenzoylarginine nitroanilidespa
dc.subject.keywordBodily secretionsspa
dc.subject.keywordChromogenic compoundsspa
dc.subject.keywordDipteraspa
dc.subject.keywordElectrophoresiseng
dc.subject.keywordEnzyme inhibitorsspa
dc.subject.keywordHumansspa
dc.subject.keywordHydrogen-ion concentrationspa
dc.subject.keywordLarvaspa
dc.subject.keywordMolecular weightspa
dc.subject.keywordPeptide hydrolasesspa
dc.subject.keywordProteolysisspa
dc.subject.keywordLarvaespa
dc.subject.keywordLarval therapyspa
dc.subject.keywordNecrotic woundsspa
dc.subject.keywordProteasesspa
dc.subject.keywordProteolytic activityspa
dc.subject.keywordSspa
dc.subject.keywordMagellanicaspa
dc.titleProteolytic activity regarding Sarconesiopsis magellanica (Diptera: Calliphoridae) larval excretions and secretionsspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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