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Draft genomes of Blastocystis subtypes from human samples of Colombia

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dc.creatorHiguera, Adrianaspa
dc.creatorSalas-Leiva, Dayana E.spa
dc.creatorCurtis, Brucespa
dc.creatorPatiño, Luz H.spa
dc.creatorZhao, Dandanspa
dc.creatorJerlström-Hultqvist, Jonspa
dc.creatorDlutek, Marlenaspa
dc.creatorMuñoz, Marinaspa
dc.creatorRoger, Andrew J.spa
dc.creatorRamírez González, Juan Davidspa
dc.date.accessioned2024-01-31T18:24:14Z
dc.date.available2024-01-31T18:24:14Z
dc.date.created2023-12-01spa
dc.date.issued2023spa
dc.descriptionBackground Blastocystis is one of the most common eukaryotic microorganisms colonizing the intestines of both humans and animals, but the conditions under which it may be a pathogen are unclear. Methods To study the genomic characteristics of circulating subtypes (ST) in Colombia, we established nine xenic cultures from Blastocystis isolated from human fecal samples, we identifed 10 diferent subtypes, since one sample had a mixed infection. Thus, the genomes of the subtypes ST1 (n=3), ST2 (n=1), ST3 (n=2), ST6 (n=1), ST7 (n=1), and ST8 (n=2) were sequenced using Illumina and Oxford Nanopore Technologies (ONT). Results Analyses of these draft nuclear genomes indicated remarkable diversity in terms of genome size and guanine-cytosine (GC) content among the compared STs. Illumina sequencing-only draft genomes contained 824 to 2077 scafolds, with total genome size ranging from 12 to 13.2 Mb and N50 values ranging from 10,585 to 29,404 base pairs (bp). The genome of one ST1 isolate was sequenced using ONT. This assembly was more contiguous, with a size of 20 million base pairs (Mb) spread over 116 scafolds, and an N50 of 248,997 bp. Conclusion This work represents one of the few large-scale comparative genomic analyses of Blastocystis isolates, providing an additional glimpse into its genomic diversity.spa
dc.format.mimetypeapplication/pdfspa
dc.identifier.doihttp://doi.org/10.1186/s13071-022-05619-7spa
dc.identifier.issn1756-3305spa
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/42112
dc.language.isoengspa
dc.publisherUniversidad del Rosariospa
dc.relation.urihttps://parasitesandvectors.biomedcentral.com/counter/pdf/10.1186/s13071-022-05619-7.pdfspa
dc.rightsAttribution 4.0 International Licensespa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccessspa
dc.rights.accesoAbierto (Texto Completo)spa
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/spa
dc.sourceParasites and Vectorsspa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subjectBlastocystisspa
dc.subjectComparative genomicsspa
dc.subjectGenomic diversityspa
dc.subjectNanopore sequencingspa
dc.titleDraft genomes of Blastocystis subtypes from human samples of Colombiaspa
dc.typearticlespa
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersionspa
dc.type.spaArtículospa
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