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Employing oxford nanopore technologies (ONT) for understanding the ecology and transmission dynamics of flaviviruses in mosquitoes (Diptera: Culicidae) from Eastern Colombia

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dc.creatorMartínez, Davidspa
dc.creatorGómez, Marcelaspa
dc.creatorDe las Salas, Jorge Luisspa
dc.creatorHernández, Carolinaspa
dc.creatorZamora Florez, Alexanderspa
dc.creatorMuñoz, Marinaspa
dc.creatorRamírez González, Juan Davidspa
dc.date.accessioned2024-01-31T18:25:21Z
dc.date.available2024-01-31T18:25:21Z
dc.date.created2023-09-01spa
dc.date.issued2023spa
dc.descriptionStudies focused on identifying the viral species of Flavivirus in vectors are scarce in Latin America and particularly in Colombia. Therefore, the frequency of infection of the Flavivirus genus and its feeding preferences were identified in the mosquito species circulating in the municipality of Puerto Carreño-Vichada, located in the Eastern Plains of Colombia. This was done by sequencing the viral NS5 and vertebrate 12S rRNA genes, respectively, using Oxford Nanopore Technologies (ONT). A total of 1,159 mosquitoes were captured, with the most abundant species being Aedes serratus at 73.6% (n = 853). All the mosquitoes were processed in 230 pools (2–6 individuals) and 51 individuals, where 37.01% (n = 104) were found to be infected with Flavivirus. In these samples, infection by arboviruses of epidemiological importance, such as dengue virus (DENV), Zika virus (ZIKV), and chikungunya virus (CHIKV), was ruled out by PCR. However, through sequencing, infection by different insect-specific viruses (ISFVs) and a medically important virus, West Nile virus (WNV), were identified in a mosquito of the Culex browni species. Additionally, the feeding patterns showed that most species present a generalist behavior. Given the above, conducting entomovirological surveillance studies is crucial, especially in areas of low anthropogenic intervention, due to the high probability that potentially pathogenic viruses could generate spillover events under deforestation scenarios.spa
dc.format.mimetypeapplication/pdfspa
dc.identifier.doihttp://doi.org/10.1016/j.actatropica.2023.106972spa
dc.identifier.issn0001-706Xspa
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/42119
dc.language.isoengspa
dc.publisherUniversidad del Rosariospa
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S0001706X23001596/pdfft?md5=7541d9d59f7c7eecfc61ccde51b3cae4&pid=1-s2.0-S0001706X23001596-main.pdfspa
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 Internationalspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccessspa
dc.rights.accesoAbierto (Texto Completo)spa
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/spa
dc.sourceActa Tropicaspa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subjectMosquitoesspa
dc.subjectFlavivirusspa
dc.subjectFeeding sourcesspa
dc.subjectInsect-specific viruses (ISFVs)spa
dc.subjectOxford nanopore technologies (ONT)spa
dc.titleEmploying oxford nanopore technologies (ONT) for understanding the ecology and transmission dynamics of flaviviruses in mosquitoes (Diptera: Culicidae) from Eastern Colombiaspa
dc.typearticlespa
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersionspa
dc.type.spaArtículospa
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