Ítem
Solo Metadatos

Bovine leukaemia virus DNA in fresh milk and raw beef for human consumption

Mostrar el registro sencillo de la publicación
dc.creatorOLAYA-GALÁN, N. N.spa
dc.creatorCORREDOR-FIGUEROA, A. P.spa
dc.creatorGUZMÁN-GARZÓN, T. C.spa
dc.creatorRÍOS-HERNANDEZ, K. S.spa
dc.creatorSALAS-CÁRDENAS, S. P.spa
dc.creatorPATARROYO, M. A.spa
dc.creatorGUTIERREZ, M. F.spa
dc.date.accessioned2020-05-26T00:07:04Z
dc.date.available2020-05-26T00:07:04Z
dc.date.created2017spa
dc.description.abstractBovine leukaemia virus (BLV) is the causative agent of enzootic bovine leucosis, which has been reported worldwide. BLV has been found recently in human tissue and it could have a significant impact on human health. A possible hypothesis regarding viral entry to humans is through the consumption of infected foodstuffs. This study was aimed at detecting the presence of BLV DNA in raw beef and fresh milk for human consumption. Nested PCR directed at the BLV gag gene (272 bp) was used as a diagnostic test. PCR products were confirmed by Sanger sequencing. Forty-nine per cent of the samples proved positive for the presence of proviral DNA. This is the first study highlighting the presence of the BLV gag gene in meat products for human consumption and confirms the presence of the viral DNA in raw milk, as in previous reports. The presence of viral DNA in food products could suggest that viral particles may also be found. Further studies are needed to confirm the presence of infected viral particles, even though the present findings could represent a first approach to BLV transmission to humans through foodstuff consumption. Copyright © Cambridge University Press 2017. This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1017/S0950268817002229
dc.identifier.issn14694409
dc.identifier.issn09502688
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23956
dc.language.isoengspa
dc.publisherCambridge University Pressspa
dc.relation.citationEndPage3130
dc.relation.citationIssueNo. 15
dc.relation.citationStartPage3125
dc.relation.citationTitleEpidemiology and Infection
dc.relation.citationVolumeVol. 145
dc.relation.ispartofEpidemiology and Infection, ISSN:14694409, 09502688, Vol.145, No.15 (2017); pp. 3125-3130spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85030853859&doi=10.1017%2fS0950268817002229&partnerID=40&md5=3da6b2b309a077eeb3bf5e9f5853ae42spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordVirus DNAspa
dc.subject.keywordDNAeng
dc.subject.keywordArticlespa
dc.subject.keywordBeefspa
dc.subject.keywordBovine leukemia virusspa
dc.subject.keywordControlled studyspa
dc.subject.keywordDNA determinationspa
dc.subject.keywordMilkspa
dc.subject.keywordNonhumanspa
dc.subject.keywordPolymerase chain reactionspa
dc.subject.keywordProvirusspa
dc.subject.keywordRaw meatspa
dc.subject.keywordSanger sequencingspa
dc.subject.keywordStructural genespa
dc.subject.keywordAnimalspa
dc.subject.keywordBovinespa
dc.subject.keywordDNA sequencespa
dc.subject.keywordEnzootic bovine leukosisspa
dc.subject.keywordGeneticsspa
dc.subject.keywordHumanspa
dc.subject.keywordMeatspa
dc.subject.keywordMilkspa
dc.subject.keywordMultiplex polymerase chain reactionspa
dc.subject.keywordTransmissionspa
dc.subject.keywordVirologyspa
dc.subject.keywordAnimalsspa
dc.subject.keywordCattlespa
dc.subject.keywordDNAeng
dc.subject.keywordEnzootic Bovine Leukosisspa
dc.subject.keywordHumansspa
dc.subject.keywordLeukemia Viruseng
dc.subject.keywordMeatspa
dc.subject.keywordMilkspa
dc.subject.keywordMultiplex Polymerase Chain Reactionspa
dc.subject.keywordPolymerase Chain Reactionspa
dc.subject.keywordSequence Analysiseng
dc.subject.keywordBovine leukaemia virusspa
dc.subject.keywordFoodborne infectionspa
dc.subject.keywordFresh Milkspa
dc.subject.keywordRaw meatspa
dc.subject.keywordZoonosisspa
dc.titleBovine leukaemia virus DNA in fresh milk and raw beef for human consumptionspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
Archivos
Colecciones
Artículos