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Plasmodium vivax: parasitemia determination by real-time quantitative PCR in Aotus monkeys

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dc.creatorPolanco, Juan Carlosspa
dc.creatorRodr??guez, Josefa Antoniaspa
dc.creatorCorredor, Vladimirspa
dc.creatorPatarroyo, Manuel A.
dc.date.accessioned2020-08-28T15:48:16Z
dc.date.available2020-08-28T15:48:16Z
dc.date.created2002-02spa
dc.description.abstractPlasmodium vivax and Plasmodium falciparum are the two prevalent human malaria species. A Colombian P. vivax wild strain has been adapted in Aotus nancymaae monkeys for use in further biological and immunological studies. We present data validating a real-time PCR assay quantifying P. vivax parasitemia, using the small subunit ribosomal RNA genes as an amplification target. P. vivax species-specific primers were designed on the 18S ribosomal gene V8 region, for amplifying both asexual and sporozoite ssrRNA genes. The assay detects amplification products bound to fluorescent SYBR-Green I dye using Perkin–Elmer GeneAmp-5700-SDS. Linear range standard curves from 6 DNA concentration logs (+0.99 correlation coefficients) were obtained. Standard curves were constructed using a plasmid containing target gene for real-time PCR amplification. This P. vivax specific assay is very sensitive, having a three parasite detection limit, and is reproducible and accurate. It involves a “closed-tube” PCR, avoids time-consuming post-PCR manipulation, and decreases potential PCR contamination.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/S0014-4894(02)00010-3
dc.identifier.issnISSN: 0014-4894
dc.identifier.issnEISSN: 1090-2449
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/28473
dc.language.isoengspa
dc.publisherElsevierspa
dc.relation.citationEndPage134
dc.relation.citationIssueNo. 2
dc.relation.citationStartPage131
dc.relation.citationTitleExperimental Parasitology
dc.relation.citationVolumeVol. 100
dc.relation.ispartofExperimental Parasitology, ISSN: 0014-4894;EISSN: 1090-2449, Vol. 100, No. 2 (February 2002); pp. 131-134spa
dc.relation.urihttps://www.sciencedirect.com/science/article/abs/pii/S0014489402000103spa
dc.rights.accesRightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.accesoRestringido (Acceso a grupos específicos)spa
dc.sourceExperimental Parasitologyspa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.keywordPlasmodium vivaxspa
dc.subject.keywordAotus nancymaaespa
dc.subject.keywordReal-time quantitativespa
dc.subject.keywordPCReng
dc.subject.keywordGreen Ispa
dc.subject.keywordParasitemiaspa
dc.subject.keywordSmall subunit ribosomal RNAspa
dc.titlePlasmodium vivax: parasitemia determination by real-time quantitative PCR in Aotus monkeysspa
dc.title.TranslatedTitlePlasmodium vivax: determinación de parasitemia mediante PCR cuantitativa en tiempo real en monos Aotusspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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