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Towards designing a synthetic antituberculosis vaccine : The Rv3587c peptide inhibits mycobacterial entry to host cells

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dc.creatorCarabali-Isajar, Mary Lilian
dc.creatorOcampo, Marisol
dc.creatorRodriguez, Deisy Carolina
dc.creatorVanegas, Magnolia
dc.creatorCurtidor, Hernando
dc.creatorPatarroyo, Manuel A.
dc.creatorPatarroyo, Manuel Elkin
dc.creator.googleCarabali-Isajar, Mary Lilian
dc.creator.googleOcampo, Marisol
dc.creator.googleRodriguez, Deisy Carolina
dc.creator.googleVanegas, Magnolia
dc.creator.googleCurtidor, Hernando
dc.creator.googlePatarroyo, Manuel Elkin
dc.date.accessioned2019-03-07T19:29:23Z
dc.date.available2019-03-07T19:29:23Z
dc.date.created2018
dc.date.issued2018
dc.description.abstractMycobacterium tuberculosis is considered one of the most successful pathogens in the history of mankind, having caused 1.7 million deaths in 2016. The amount of resistant and extensively resistant strains has increased; BCG has been the only vaccine to be produced in more than 100 years though it is still unable to prevent the disease's most disseminated form in adults; pulmonary tuberculosis. The search is thus still on-going for candidate antigens for an antituberculosis vaccine. This paper reports the use of a logical and rational methodology for finding such antigens, this time as peptides derived from the Rv3587c membrane protein. Bioinformatics tools were used for predicting mycobacterial surface location and Rv3587c protein structure whilst circular dichroism was used for determining its peptides’ secondary structure. Receptor-ligand assays identified 4 high activity binding peptides (HABPs) binding specifically to A549 alveolar epithelial cells and U937 monocyte-derived macrophages, covering the region between amino acids 116 and 193. Their capability for inhibiting Mtb H37Rv invasion was evaluated. The recognition of antibodies from individuals suffering active and latent tuberculosis and from healthy individuals was observed in HABPs capable of avoiding mycobacterial entry to host cells. The results showed that 8 HABPs inhibited such invasion, two of them being common for both cell lines: 39265 (155VLAAYVYSLDNKRLWSNLDT173) and 39266 (174APSNETLVKTFSPGEQVTTY192). Peptide 39265 was the least recognised by antibodies from the individuals’ sera evaluated in each group. According to the model proposed by FIDIC regarding synthetic vaccine development, peptide 39265 has become a candidate antigen for an antituberculosis vaccine. © 2018 The Authorseng
dc.format.mimetypeapplication/pdf
dc.identifier.doi10.1016/j.bmc.2018.03.044
dc.identifier.issn0968-0896
dc.identifier.urihttp://repository.urosario.edu.co/handle/10336/19225
dc.language.isoengspa
dc.relation.citationEndPage2409
dc.relation.citationStartPage2401
dc.relation.citationTitleBioorganic and Medicinal Chemistry
dc.relation.citationVolumeVol. 26
dc.relation.ispartofBioorganic and Medicinal Chemistry, ISSN:9680-896, Vol. 26 (2018) pp. 2401-2409spa
dc.relation.urihttps://reader.elsevier.com/reader/sd/pii/S0968089617324872?token=4252B3E4421EBCB5AAE05A6C580905767E614D2FF5579797338849FF9A8FFAF6BF7945323F4DC5A642BC0CF5F8973C85spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.bibliographicCitation(2017), WHO, World Health Organisation. Global tuberculosis report, 2017spa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subjectBcg Vaccinespa
dc.subjectMembrane Proteinspa
dc.subjectPeptide 39265spa
dc.subjectPeptide 39266spa
dc.subjectUnclassified Drugspa
dc.subjectAntibody Detectionspa
dc.subjectBioinformaticsspa
dc.subjectCircular Dichroismspa
dc.subjectControlled Studyspa
dc.subjectDrug Designspa
dc.subjectHost Pathogen Interactionspa
dc.subjectHumanspa
dc.subjectLatent Tuberculosisspa
dc.subjectLung Alveolus Epithelium Cellspa
dc.subjectMacrophagespa
dc.subjectMethodologyspa
dc.subjectMonocyte Derived Macrophagespa
dc.subjectMycobacteriumspa
dc.subjectMycobacterium Tuberculosisspa
dc.subjectMycobacterium Tuberculosis H37Rvspa
dc.subjectNonhumanspa
dc.subjectPromoter Regionspa
dc.subjectProtein Bindingspa
dc.subjectProtein Secondary Structurespa
dc.subjectProtein Structurespa
dc.subjectTuberculosisspa
dc.subjectVacuna BCGspa
dc.subjectProteínas de membranaspa
dc.subjectPéptido 39 265spa
dc.subject39266 péptidospa
dc.subject.ddcEnfermedadesspa
dc.subject.keywordArticlespa
dc.subject.lembMycobacterium tuberculosisspa
dc.subject.lembAntígenos bacterianosspa
dc.titleTowards designing a synthetic antituberculosis vaccine : The Rv3587c peptide inhibits mycobacterial entry to host cellsspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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