Ítem
Acceso Abierto

Diagnostic accuracy of combinations of serological biomarkers for identifying clinical tuberculosis

Mostrar el registro sencillo de la publicación
dc.creatorAraujo, Z.
dc.creatorMacias-Segura, N.
dc.creatorLópez-Ramos, J.E.
dc.creatorde Waard, J.H.
dc.creatorVanegas, M.
dc.creatorPatarroyo, M.A.
dc.creatorSalgado, A.
dc.creatorEnciso-Moreno, J.A.
dc.creatorAraujo, Zaida
dc.creatorMacias-Segura, Noe
dc.creatorLópez-Ramos, Juan E.
dc.creatorde Waard, Jacobus H.
dc.creatorVanegas, Magnolia
dc.creatorPatarroyo, Manuel A.
dc.creatorSalgado, Antonio
dc.creatorEnciso-Moreno, Jose Antonio
dc.creator.googleAraujo, Z., Macias-Segura, N., López-Ramos, J.E., de Waard, J.H., Vanegas, M., Patarroyo, M.A., Salgado, A., Enciso-Moreno, J.A.spa
dc.date.accessioned2019-09-24T15:43:30Z
dc.date.available2019-09-24T15:43:30Z
dc.date.created2018
dc.date.issued2018
dc.description.abstractIntroduction: Confirmation of tuberculosis (TB) cases in endemic TB settings is a challenge; obtaining fast and cheap, though accurate, diagnostic tools such as biomarkers is thus urgently needed to enable the early detection of TB. This paper evaluates the diagnostic accuracy of combinations of host serological biomarkers for identifying TB. Methodology: Enzyme-linked immunosorbent assays (ELISA) were used on 70 Venezuelan Creole individuals for evaluating host biomarkers (i.e. CXCL9, sCD14, MMP9 and uPAR proteins) and anti-synthetic peptides covering certain Mycobacterium tuberculosis (Mtb) ESAT-6 (P-12033, P-12034 and P-12037) and Ag85A (P-29878) antigen sequences. The target population consisted of adults having active TB (ATB, n = 28), the tuberculin skin test positive (TST+) or individuals with latent TB infection (LTB, n = 28) and TST- or control subjects (CTRL, n = 14). Results: Receiver operator curve (ROC) analysis revealed good biosignature discriminative ability for 5 serological biomarkers; the accuracy of 3 combinations had a good discriminative ability for diagnosing TB. Anti-P-12034/uPAR detected TB with 96.7% sensitivity and 86.0% specificity, followed by anti-P-12033/uPAR having 96.7% sensitivity and 81.4% specificity. Anti-P-29878/MMP9 had the highest sensitivity (100%), but low specificity (52.17%). Biomarker combinations did not prove efficacious for identifying incipient subclinical TST+TB− subjects at high-risk for TB. Conclusions: The anti-P-12034/uPAR combination could be useful for identifying clinical TB patients. Such an approach holds promise for further validation. © 2018 Araujo et al.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doi10.3855/jidc.9554
dc.identifier.issn2036-6590
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/20325
dc.language.isoengspa
dc.relation.citationEndPage441
dc.relation.citationStartPage429
dc.relation.citationTitleJournal of Infection in Developing Countries
dc.relation.citationVolumeVol. 12
dc.relation.ispartofJournal of Infection in Developing Countries, ISSN:2036-6590, Vol. 12 (2018) pp. 429-441spa
dc.relation.urihttps://jidc.org/index.php/journal/article/view/9554/1891spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.bibliographicCitation(2016) Global Tuberculosis Report 2016, , http://www.who.int/tb/publications/global_report/en/, Accessed:12 October 2016)spa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subjectAnti P 12033spa
dc.subjectAnti P 12034spa
dc.subjectAnti P 12037spa
dc.subjectAnti P 29878spa
dc.subjectBiological Markerspa
dc.subjectCd14 Antigenspa
dc.subjectCxcl9 Chemokinespa
dc.subjectEarly Secretory Antigenic Target 6spa
dc.subjectGelatinase Bspa
dc.subjectImmunoglobulin Gspa
dc.subjectPeptide Antibodyspa
dc.subjectSynthetic Peptidespa
dc.subjectUnclassified Drugspa
dc.subjectUrokinase Receptorspa
dc.subjectAmino Acid Sequencespa
dc.subjectArea Under The Curvespa
dc.subjectBacterium Culturespa
dc.subjectBlood Examinationspa
dc.subjectClinical Featurespa
dc.subjectControlled Studyspa
dc.subjectDelayed Hypersensitivityspa
dc.subjectDiagnostic Accuracyspa
dc.subjectDiagnostic Test Accuracy Studyspa
dc.subjectEnzyme Linked Immunosorbent Assayspa
dc.subjectHumanspa
dc.subjectImmunoreactivityspa
dc.subjectLatent Tuberculosisspa
dc.subjectMajor Clinical Studyspa
dc.subjectMalespa
dc.subjectMycobacterium Tuberculosisspa
dc.subjectPredictive Valuespa
dc.subjectReceiver Operating Characteristicspa
dc.subjectSensitivity And Specificityspa
dc.subjectSputum Analysisspa
dc.subjectStainingspa
dc.subjectThorax Radiographyspa
dc.subjectTuberculin Testspa
dc.subjectTuberculosisspa
dc.subjectX Rayspa
dc.subject.ddcBiologíaspa
dc.subject.keywordBacterial antigenspa
dc.subject.keywordCd14 Antigenspa
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordAdultspa
dc.subject.keywordArticlespa
dc.subject.keywordFemalespa
dc.subject.lembMarcadores bioquímicosspa
dc.titleDiagnostic accuracy of combinations of serological biomarkers for identifying clinical tuberculosisspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
Archivos
Bloque original
Mostrando1 - 1 de 1
Miniatura
Nombre:
52.pdf
Tamaño:
859.89 KB
Formato:
Adobe Portable Document Format
Descripción:
Descargar
Colecciones
Artículos