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Molecular epidemiology of entamoeba : First description of Entamoeba moshkovskii in a rural area from central Colombia

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dc.creatorLópez, Myriam Consuelo
dc.creatorLeón, Cielo M.
dc.creatorFonseca, Jairo
dc.creatorReyes, Patricia
dc.creatorMoncada, Ligia
dc.creatorOlivera, Mario J.
dc.creatorRamírez, Juan David
dc.creator.googleLópez, Myriam Consuelo
dc.creator.googleLeón, Cielo M.
dc.creator.googleFonseca, Jairo
dc.creator.googleReyes, Patricia
dc.creator.googleMoncada, Ligia
dc.creator.googleOlivera, Mario J.
dc.creator.googleRamírez, Juan David
dc.date.accessioned2019-02-06T15:26:00Z
dc.date.available2019-02-06T15:26:00Z
dc.date.created2015
dc.date.issued2015
dc.description.abstractBackground Entamoeba histolytica, E. dispar and E. moshkovskii are the most frequent species described in human infection where E. histolytica is the only true pathogen. The epidemiology of this infection is complex due to the absence of a routine exam that allows a correct discrimination of the Entamoeba species complex. Therefore, molecular methods appear as the unique epidemiological tool to accomplish the species discrimination. Herein, we conducted a cross-sectional study to determine the frequency of Entamoeba species infections in a group of asymptomatic individuals from a rural area in central Colombia. Methodology/Principal Findings A total of 181 fecal samples from asymptomatic children under 16 years old from the hamlet La Vírgen, Cundinamarca (Colombia) that voluntarily accepted to participate in the study were collected. The fecal samples were examined by light microscopy and DNA-extracted, subsequently submitted to molecular discrimination of E. dispar/E. histolytica/E. moshkovskii infection based on a multiplex PCR assay targeting the 18S rRNA fragment. To confirm the species description, twenty samples were randomly submitted to DNA sequencing of the aforementioned fragment. By direct microscopic examination, frequency of the complex E. histolytica/E. dispar/E. moshkovskii was 18.8%(34/181). PCR showed a frequency of 49.1% (89/181), discriminated as 23.2%(42/181) that were positive for E. dispar, 25.4% (46/181) for E. moshkovskii and 0.55% (1/ 181) for E. histolytica. Also, mixed infections were detected between E. dispar and E. moshkovskii at 4.42% (8/181) of the samples. Molecular barcoding confirmed the diagnosis depicted by the multiplex PCR assay. Conclusions/Significance This is the first description of E. moshkovskii in Colombia and the second report in South- America to our knowledge. Our results suggest the need to unravel the true epidemiology of Entamoeba infections around the world, including the real pathogenic role that E. moshkovskii may have. Copyright: © 2015 López et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0140302
dc.identifier.issnISSN 1932-6203
dc.identifier.urihttp://repository.urosario.edu.co/handle/10336/19018
dc.language.isoengspa
dc.relation.citationIssueNo. 10
dc.relation.citationTitlePLoS ONE
dc.relation.citationVolumeVol. 10
dc.relation.ispartofPLoS ONE, ISSN: 1932-6203, Vol. 10/No. 10 (2015)spa
dc.relation.urihttps://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0140302&type=printablespa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.rights.cchttps://creativecommons.org/licenses/by/4.0/spa
dc.source.bibliographicCitationWHO/PAHO/UNESCO report of a consultation with experts on amoebiasis (1997) Epidemiol Bull., 18, pp. 13-14spa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subjectARN 18Cspa
dc.subjectAdolescentespa
dc.subjectAmebiasisspa
dc.subjectInfección amebianaspa
dc.subjectInfección asintomáticaspa
dc.subjectNiñospa
dc.subjectEstudio transversalspa
dc.subjectExtracción de ADNspa
dc.subjectSecuencia de ADNspa
dc.subjectEntamoeba Disparspa
dc.subjectEntamoeba Dispar Infectionspa
dc.subjectInfección por Entamoeba muscovensisspa
dc.subjectAnálisis de hecesspa
dc.subjectHumanospa
dc.subjectEstudio clínico principalspa
dc.subjectMasculinospa
dc.subjectMicroscopíaspa
dc.subjectInfección mixtaspa
dc.subjectEpidemiología Molecularspa
dc.subjectReacción en cadena de la polimerasaspa
dc.subjectÁrea ruralspa
dc.subjectClasificaciónspa
dc.subjectEntamoebaspa
dc.subjectHecesspa
dc.subjectGenéticaspa
dc.subjectRecién nacidospa
dc.subjectParasitologíaspa
dc.subjectFilogeniaspa
dc.subjectNiño preescolarspa
dc.subjectPredominiospa
dc.subjectPoblación ruralspa
dc.subjectCoinfecciónspa
dc.subject.ddcIncidencia & prevención de la enfermedadspa
dc.subject.keywordAdulteng
dc.subject.keywordCoinfectioneng
dc.subject.keywordFemaleeng
dc.subject.keywordHumanseng
dc.subject.keywordEntamoeba Moshkovskii Infectionben
dc.subject.keywordRural Populationeng
dc.subject.keywordPrevalenceeng
dc.subject.keywordPreschool Childeng
dc.subject.keywordPhylogenyeng
dc.subject.keywordParasitologyeng
dc.subject.keywordNewborneng
dc.subject.keywordGeneticseng
dc.subject.keywordClassificationeng
dc.subject.keywordFeceseng
dc.subject.keywordRural Areaeng
dc.subject.keywordPolymerase Chain Reactioneng
dc.subject.keywordMolecular Epidemiologyeng
dc.subject.keywordMicroscopyeng
dc.subject.keywordMixed Infectioneng
dc.subject.keywordMajor Clinical Studyeng
dc.subject.keywordMaleeng
dc.subject.keywordHumaneng
dc.subject.keywordAdolescenteng
dc.subject.keywordFeces Analysiseng
dc.subject.keywordDna Sequenceeng
dc.subject.keywordDna Extractioneng
dc.subject.keywordCross-Sectional Studyeng
dc.subject.keywordChildeng
dc.subject.keywordAsymptomatic Infectioneng
dc.subject.keywordAmebic Infectioneng
dc.subject.keywordAmebiasiseng
dc.subject.keywordRna 18Seng
dc.subject.lembEpidemiologíaspa
dc.subject.lembInfeccionesspa
dc.subject.lembEntamoebaspa
dc.titleMolecular epidemiology of entamoeba : First description of Entamoeba moshkovskii in a rural area from central Colombiaspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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