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High-throughput analysis of the Trypanosoma cruzi minicirculome (mcDNA) unveils structural variation and functional diversity

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dc.creatorGómez‑Palacio, Andrésspa
dc.creatorCruz-Saavedra, Lissaspa
dc.creatorVan den Broeck, Frederikspa
dc.creatorGeerts, Manonspa
dc.creatorPita, Sebastiánspa
dc.creatorVallejo, Gustavo A.spa
dc.creatorCarranza, Julio C.spa
dc.creatorRamírez González, Juan Davidspa
dc.date.accessioned2025-01-26T18:31:00Z
dc.date.available2025-01-26T18:31:00Z
dc.date.created2024-12-01spa
dc.date.issued2024-12-01spa
dc.descriptionTrypanosoma cruzi causes Chagas disease and has a unique extranuclear genome enclosed in a structure called the kinetoplast, which contains circular genomes known as maxi- and minicircles. While the structure and function of maxicircles are well-understood, many aspects of minicircles remain to be discovered. Here, we performed a high-throughput analysis of the minicirculome (mcDNA) in 50 clones isolated from Colombia’s diverse T. cruzi I populations. Results indicate that mcDNA comprises four diverse subpopulations with different structures, lengths, and numbers of interspersed semi-conserved (previously termed ultra-conserved regions mHCV) and hypervariable (mHVPs) regions. Analysis of mcDNA ancestry and inter-clone differentiation indicates the interbreeding of minicircle sequence classes is placed along diverse strains and hosts. These results support evidence of the multiclonal dynamics and random bi-parental segregation. Finally, we disclosed the guide RNA repertoire encoded by mcDNA at a clonal scale, and several attributes of its abundance and function are discussed.spa
dc.format.mimetypeapplication/pdfspa
dc.identifier.doihttps://doi.org/10.1038/s41598-024-56076-4spa
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/44813
dc.language.isoengspa
dc.publisherScientific Reportsspa
dc.relation.ispartofScientific Reportsspa
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccessspa
dc.rights.accesoAbierto (Texto Completo)spa
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/spa
dc.sourceScientific Reportsspa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subjectTrypanosoma cruzispa
dc.subjectChagas diseasespa
dc.subjectColombiaspa
dc.subjectGuide RNAspa
dc.subjectMaxicirclesspa
dc.subjectMinicirclesspa
dc.subjectkDNAspa
dc.titleHigh-throughput analysis of the Trypanosoma cruzi minicirculome (mcDNA) unveils structural variation and functional diversityspa
dc.typearticlespa
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersionspa
dc.type.spaArtículospa
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