Ítem
Acceso Abierto

Interaction of leishmania (L.) chagasi with the lulo cell line in different environmental conditions

Mostrar el registro sencillo de la publicación
dc.creatorAcero V.spa
dc.creatorGaleano E.spa
dc.creatorAyala M.spa
dc.creatorCastellanos J.spa
dc.creatorBello F.spa
dc.date.accessioned2020-05-25T23:59:13Z
dc.date.available2020-05-25T23:59:13Z
dc.date.created2006spa
dc.description.abstractThere are unknown or poorly understood events in the Leishmania-host cell interactions that merit studies in new in vitro models. The aim of the present work was to evaluate Leishmania chagasi interaction with a cell line (Lulo) previously established and derived from Lutzomyia longipalpis embryonic tissues, bearing different temperatures and CO 2 concentrations in mind and morphometric changes of the cells. The J774 cell line was used as positive control of the infection. The L. chagasi MH/CO/84/CL-044B strain was used for infection assays. The parasites were added to adherent cells, in Lulo as well as J774, in a 5:1 ratio. Incubation was at 28°C and 37°C, in the absence and presence of CO 2. A morphometric study of the cells was carried out before and after infection. Both parasite and cell ultrastructure characteristics were analyzed by electron microscope. The greatest percentage of infection in the Lulo cells at 28°C (26,8%) was registered on day six post infection, while at 37°C, in the absence of CO 2, the maximum value (30,4%) was obtained on day nine. The morphometric analyses showed a significant increase in the size of Lulo cells infected at 28°C and J774 cells at 37°C with and without CO 2, respectively.spa
dc.format.mimetypeapplication/pdf
dc.identifier.issn1200488
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23007
dc.language.isospaspa
dc.relation.citationEndPage171
dc.relation.citationIssueNo. 2
dc.relation.citationStartPage165
dc.relation.citationTitleRevista Colombiana de Entomologia
dc.relation.citationVolumeVol. 32
dc.relation.ispartofRevista Colombiana de Entomologia, ISSN:1200488, Vol.32, No.2 (2006); pp. 165-171spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-79960469168&partnerID=40&md5=3c70dbc529752606952e463511204368spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordLeishmania donovani chagasispa
dc.subject.keywordLutzomyia longipalpisspa
dc.subject.keywordSolanum quitoensespa
dc.subject.keywordCell morphometryspa
dc.subject.keywordPercentage of infectionspa
dc.subject.keywordPromastigotespa
dc.subject.keywordTemperature and co 2spa
dc.subject.keywordUltrastructure characteristicsspa
dc.titleInteraction of leishmania (L.) chagasi with the lulo cell line in different environmental conditionsspa
dc.title.TranslatedTitleInteracción de leishmania (L.) chagasi con la línea celular lulo en diferentes condiciones ambientalesspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
Archivos
Bloque original
Mostrando1 - 1 de 1
Miniatura
Nombre:
v32n2a10.pdf
Tamaño:
547.84 KB
Formato:
Adobe Portable Document Format
Descripción:
Descargar
Colecciones
Artículos