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Amino terminal peptides from the Plasmodium falciparum EBA-181/JESEBL protein bind specifically to erythrocytes and inhibit in vitro merozoite invasion

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dc.creatorVera-Bravo, Ricardospa
dc.creatorValbuena, John J.spa
dc.creatorOcampo, Marisol
dc.creatorGarcia, Javier E.spa
dc.creatorRodriguez, Luis E.spa
dc.creatorPuentes, Alvarospa
dc.creatorLopez, Ramsesspa
dc.creatorCurtidor, Hernandospa
dc.creatorTorres, Elizabethspa
dc.creatorTrujillo, Maryspa
dc.creatorTovar, Diana R.spa
dc.creatorPatarroyo, Manuel A.spa
dc.creatorPatarroyo, Manuel E.spa
dc.date.accessioned2020-08-06T16:20:06Z
dc.date.available2020-08-06T16:20:06Z
dc.date.created2005-05spa
dc.description.abstractSeveral EBA-175 paralogues (EBA-140, EBA-165, EBA-175, EBA-181, and EBL-1) have been described among the Plasmodium falciparum malaria parasite proteins, which are important in the red blood cell (RBC) invasion process. EBA-181/JESEBL is a 181 kDa protein expressed in the late schizont stage and located in the micronemes; it belongs to the Plasmodium Duffy binding-like family and is able to interact with the erythrocyte surface. Here, we describe the synthesis of 78, 20-mer synthetic peptides derived from the reported EBA-181/JESEBL sequence and their ability to bind RBCs in receptor–ligand assays. Five peptides (numbered 30030, 30031, 30045, 30051, and 30060) displayed high specific binding to erythrocytes; their equilibrium binding parameters were then determined. These peptides interacted with 53 and 33 kDa receptor proteins on the erythrocyte surface, this binding being altered when RBCs were pretreated with enzymes. They were able to inhibit P. falciparum merozoite invasion of RBCs when tested in in vitro assays. According to these results, these five EBA-181/JESEBL high specific erythrocyte binding peptides, as well as the entire protein, were seen to be involved in the molecular machinery used by the parasite for invading RBCs. They are thus suggested as potential candidates in designing a multi-sub-unit vaccine able to combat the P. falciparum malaria parasite.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.biochi.2005.01.005
dc.identifier.issnISSN: 0300-9084
dc.identifier.issnEISSN: 6183-1638
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/25878
dc.language.isoengspa
dc.publisherElsevierspa
dc.relation.citationEndPage436
dc.relation.citationIssueNo. 5
dc.relation.citationStartPage425
dc.relation.citationTitleBiochimie
dc.relation.citationVolumeVol. 87
dc.relation.ispartofBiochimie, ISSN: 0300-9084;EISSN: 6183-1638, Vol.87 No.5 (2005); pp.425-436 spa
dc.relation.urihttps://www.sciencedirect.com/science/article/abs/pii/S0300908405000118?via%3Dihubspa
dc.rights.accesRightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.accesoRestringido (Acceso a grupos específicos)spa
dc.sourceBiochimiespa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.keywordEBA-181spa
dc.subject.keywordPlasmodium falciparumspa
dc.subject.keywordSynthetic peptidespa
dc.subject.keywordBinding assayspa
dc.subject.keywordMalariaspa
dc.titleAmino terminal peptides from the Plasmodium falciparum EBA-181/JESEBL protein bind specifically to erythrocytes and inhibit in vitro merozoite invasionspa
dc.title.TranslatedTitleLos péptidos amino terminales de la proteína Plasmodium falciparum EBA-181 / JESEBL se unen específicamente a los eritrocitos e inhiben la invasión in vitro de merozoitosspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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