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Multicenter validation of commercial antigenuria reagents to diagnose progressive disseminated histoplasmosis in people living with HIV/AIDS in two Latin American countries

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dc.creatorCáceres, Diego H.spa
dc.creatorSamayoa, Blanca E.spa
dc.creatorMedina, Narda G.spa
dc.creatorTobón, Angela M.spa
dc.creatorGuzmán, Brenda J.spa
dc.creatorMercado, Danicelaspa
dc.creatorRestrepo, Angelaspa
dc.creatorChiller, Tomspa
dc.creatorArathoon, Eduardo E.spa
dc.creatorGómez, Beatriz L.spa
dc.date.accessioned2020-05-25T23:56:03Z
dc.date.available2020-05-25T23:56:03Z
dc.date.created2018spa
dc.description.abstractHistoplasmosis is an important cause of mortality in patients with AIDS, especially in countries with limited access to antiretroviral therapies and diagnostic tests. However, many disseminated infections in Latin America go undiagnosed. A simple, rapid method to detect Histoplasma capsulatum infection in regions where histoplasmosis is endemic would dramatically decrease the time to diagnosis and treatment, reducing morbidity and mortality. The aim of this study was to validate a commercial monoclonal Histoplasma galactomannan (HGM) enzyme-linked immunosorbent assay (Immuno-Mycologics [IMMY], Norman, OK, USA) in two cohorts of people living with HIV/AIDS (PLHIV). We analyzed urine samples from 589 people (466 from Guatemala and 123 from Colombia), including 546 from PLHIV and 43 from non-PLHIV controls. Sixty-three of these people (35 from Guatemala and 28 from Colombia) had confirmed histoplasmosis by isolation of H. capsulatum. Using the standard curve provided by the quantitative commercial test, the sensitivity was 98% (95% confidence interval [CI], 95 to 100%) and the specificity was 97% (95% CI, 96 to 99%) (cutoff 0.5 ng/ml). Semiquantitative results, using a calibrator of 12.5 ng/ml of Histoplasma galactomannan to calculate an enzyme immunoassay index value (EIV) for the samples, showed a sensitivity of 95% (95% CI, 89 to 100%) and a specificity of 98% (95% CI, 96 to 99%) (cutoff 2.6 EIV). This relatively simple-to-perform commercial antigenuria test showed a high performance with reproducible results in both countries, suggesting that it can be used to detect progressive disseminated histoplasmosis in PLHIV in a wide range of clinical laboratories in countries where histoplasmosis is endemic. Copyright © 2018 American Society for Microbiology. All Rights Reserved.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1128/JCM.01959-17
dc.identifier.issn1098660X
dc.identifier.issn00951137
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22303
dc.language.isoengspa
dc.publisherAmerican Society for Microbiologyspa
dc.relation.citationIssueNo. 6
dc.relation.citationTitleJournal of Clinical Microbiology
dc.relation.citationVolumeVol. 56
dc.relation.ispartofJournal of Clinical Microbiology, ISSN:1098660X, 00951137, Vol.56, No.6 (2018)spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85047933858&doi=10.1128%2fJCM.01959-17&partnerID=40&md5=b4ab1c868d726a64b9bdd50bce255369spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordGalactomannanspa
dc.subject.keywordFungus antigenspa
dc.subject.keywordMannanspa
dc.subject.keywordAcquired immune deficiency syndromespa
dc.subject.keywordArticlespa
dc.subject.keywordCohort analysisspa
dc.subject.keywordColombiaspa
dc.subject.keywordControlled studyspa
dc.subject.keywordDiagnostic accuracyspa
dc.subject.keywordDiagnostic test accuracy studyspa
dc.subject.keywordDiagnostic valuespa
dc.subject.keywordEnzyme linked immunosorbent assayspa
dc.subject.keywordFungal detectionspa
dc.subject.keywordFungus isolationspa
dc.subject.keywordGuatemalaspa
dc.subject.keywordHistoplasmosisspa
dc.subject.keywordHumanspa
dc.subject.keywordHuman immunodeficiency virus infectionspa
dc.subject.keywordMajor clinical studyspa
dc.subject.keywordNonhumanspa
dc.subject.keywordPredictive valuespa
dc.subject.keywordPriority journalspa
dc.subject.keywordProgressive disseminated histoplasmosisspa
dc.subject.keywordReproducibilityspa
dc.subject.keywordRetrospective studyspa
dc.subject.keywordSensitivity and specificityspa
dc.subject.keywordSouth and central americaspa
dc.subject.keywordUrine samplingspa
dc.subject.keywordAids related complexspa
dc.subject.keywordClinical trialspa
dc.subject.keywordComplicationspa
dc.subject.keywordDiagnostic kitspa
dc.subject.keywordEnzyme linked immunosorbent assayspa
dc.subject.keywordHispanicspa
dc.subject.keywordHistoplasmaspa
dc.subject.keywordHistoplasmosisspa
dc.subject.keywordImmunologyspa
dc.subject.keywordIsolation and purificationspa
dc.subject.keywordMicrobiologyspa
dc.subject.keywordMixed infectionspa
dc.subject.keywordMulticenter studyspa
dc.subject.keywordUrinespa
dc.subject.keywordValidation studyspa
dc.subject.keywordVirologyspa
dc.subject.keywordAids-related opportunistic infectionsspa
dc.subject.keywordAntigenseng
dc.subject.keywordCohort studiesspa
dc.subject.keywordCoinfectionspa
dc.subject.keywordColombiaspa
dc.subject.keywordEnzyme-linked immunosorbent assayspa
dc.subject.keywordGuatemalaspa
dc.subject.keywordHispanic americansspa
dc.subject.keywordHistoplasmaspa
dc.subject.keywordHistoplasmosisspa
dc.subject.keywordMannansspa
dc.subject.keywordReagent kitseng
dc.subject.keywordReproducibility of resultsspa
dc.subject.keywordRetrospective studiesspa
dc.subject.keywordSensitivity and specificityspa
dc.subject.keywordAidsspa
dc.subject.keywordAntigenspa
dc.subject.keywordElisaspa
dc.subject.keywordHistoplasma capsulatumspa
dc.subject.keywordHistoplasmosisspa
dc.titleMulticenter validation of commercial antigenuria reagents to diagnose progressive disseminated histoplasmosis in people living with HIV/AIDS in two Latin American countriesspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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