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Lulo cell line derived from Lutzomyia longipalpis (Diptera: Psychodidae) : A novel model to assay Leishmania spp. and vector interaction

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dc.creatorCôrtes, Luzia MC
dc.creatorSilva, Roger MM
dc.creatorPereira, Bernardo AS
dc.creatorGuerra, Camila
dc.creatorZapata, Angela C
dc.creatorBello, Felio J.
dc.creatorFinkelstein, Léa C
dc.creatorMadeira, Maria F
dc.creatorBrazil, Reginaldo P
dc.creatorCôrte-Real, Suzana
dc.creatorAlves, Carlos R
dc.creator.googleCôrtes, Luzia MCspa
dc.creator.googleSilva, Roger MMspa
dc.creator.googlePereira, Bernardo ASspa
dc.creator.googleGuerra, Camilaspa
dc.creator.googleZapata, Angela Cspa
dc.creator.googleBello, Felio Jspa
dc.creator.googleFinkelstein, Léa Cspa
dc.creator.googleMadeira, Maria Fspa
dc.creator.googleBrazil, Reginaldo Pspa
dc.creator.googleCôrte-Real, Suzanaspa
dc.creator.googleAlves, Carlos Rspa
dc.date.accessioned2020-05-07T14:45:54Z
dc.date.available2020-05-07T14:45:54Z
dc.date.created2011
dc.date.issued2011
dc.description.abstractBackground: Leishmania (Vianna) braziliensis, Leishmania (Leishmania) amazonensis and Leishmania (Leishmania) chagasi are important parasites in the scenario of leishmaniasis in Brazil. During the life cycle of these parasites, the promastigote forms adhere to the midgut epithelial microvillii of phlebotomine insects to avoid being secreted along with digestive products. Lulo cells are a potential model that will help to understand the features of this adhesion phenomenon. Here, we analyze the interaction between Leishmania spp. promastigotes and Lulo cells in vitro, specifically focusing on adhesion events occurring between three Leishmania species and this cell line. Methods. Confluent monolayers of Lulo cells were incubated with promastigotes and adhesion was assessed using both light microscopy and scanning electron microscopy. Findings. The results indicate that species from the subgenera Leishmania and Viannia have great potential to adhere to Lulo cells. The highest adherence rate was observed for L. (L.) chagasi after 24 h of incubation with Lulo cells (27.3 1.8% of cells with adhered promastigotes), followed by L. (L.) amazonensis (16.0 0.7%) and L. (V.) braziliensis (3.0 0.7%), both after 48 h. In the ultrastructural analysis, promastigote adherence was also assessed by scanning electron microscopy, showing that, for parasites from both subgenera, adhesion occurs by both the body and the flagellum. The interaction of Lulo cells with Leishmania (L.) chagasi showed the participation of cytoplasmic projections from the former closely associating the parasites with the cells. Conclusions: We present evidence that Lulo cells can be useful in studies of insect-parasite interactions for Leishmania species. © 2011 Côrtes et al; licensee BioMed Central Ltd.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1186/1756-3305-4-216
dc.identifier.issn1756-3305
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/21894
dc.language.isoengspa
dc.relation.citationIssueNo. 1
dc.relation.citationTitleParasites and Vectors
dc.relation.citationVolumeVol. 4
dc.relation.ispartofParasites and Vectors, ISSN: 1756-3305 Vol. 4, No. 1 (2011)spa
dc.relation.urihttps://parasitesandvectors.biomedcentral.com/articles/10.1186/1756-3305-4-216spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.ddcEnfermedadesspa
dc.subject.ddcMicrobiologíaspa
dc.subject.keywordLeishmania sppspa
dc.subject.keywordLulo cellspa
dc.subject.keywordPhlebotominaespa
dc.subject.keywordPromastigotesspa
dc.titleLulo cell line derived from Lutzomyia longipalpis (Diptera: Psychodidae) : A novel model to assay Leishmania spp. and vector interactionspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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