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Extraction and Optical Analysis of the Extracellular Fluid from the Body Segments of Apis mellifera Bees

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dc.creatorNisperuza, J.spa
dc.creatorFayad, R.spa
dc.creatorRiveros Rivera, Andre Josafatspa
dc.creatorMesa, F.spa
dc.creatorHernández, J.spa
dc.date.accessioned2025-07-21T16:42:37Z
dc.date.available2025-07-21T16:42:37Z
dc.date.created2025-05-09spa
dc.date.issued2025-05-09spa
dc.description.abstractThis study developed and optimized a methodology based on controlled centrifugation for the segmented extraction of extracellular fluid in Apis mellifera bees. Three critical variables were analyzed: relative centrifugal force, centrifugation time, and the number of individuals processed, ensuring the reproducibility and efficiency of the procedure. The results demonstrated significant differences in the volume of fluid recovered from different body segments, with the abdomen yielding the highest volumes, followed by the thorax and the head. UV-Vis spectroscopic characterization revealed distinct optical features for the samples, identifying specific absorbance peaks unique to each segment. Furthermore, biochemical analysis using Benedict's reagent confirmed the presence of reducing sugars, with head samples displaying the most intense coloration. These findings underscore the importance of segment-specific analysis to gain deeper insights into the physiology and metabolism of bees. The proposed methodology offers a novel and robust tool for physiological, metabolic, and ecotoxicological studies, facilitating the assessment of environmental and contaminant impacts on pollinator health.eng
dc.format.mimetypeapplication/pdfspa
dc.identifier.doihttps://doi.org/10.1093/iob/obaf018spa
dc.identifier.issn2517-4843spa
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/46066
dc.language.isoengspa
dc.publisherOxford Academicspa
dc.relation.ispartofIntegrative Organismal Biology Volume 7, Issue 1 2025spa
dc.relation.urihttps://academic.oup.com/iob/article/7/1/obaf018/8128015spa
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccessspa
dc.rights.accesoAbierto (Texto Completo)spa
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/spa
dc.sourceIntegrative Organismal Biologyspa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordApis melliferaeng
dc.subject.keywordControlled centrifugationeng
dc.subject.keywordExtracellular fluideng
dc.subject.keywordSegment-specific analysiseng
dc.subject.keywordUV-Vis spectroscopyeng
dc.subject.keywordBiochemical analysiseng
dc.subject.keywordReducing sugarseng
dc.subject.keywordPollinator healtheng
dc.subject.keywordEcotoxicological studieseng
dc.titleExtraction and Optical Analysis of the Extracellular Fluid from the Body Segments of Apis mellifera Beesspa
dc.typearticlespa
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersionspa
dc.type.spaArtículo de Investigaciónspa
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