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Aplication of authenticity criteria in mitochondrial studies on archaic bone remains from a prehispanic Muisca population

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dc.creatorJara N.P.spa
dc.creatorDíaz M.spa
dc.creatorVillegas Gálvez, Victoria Eugeniaspa
dc.creatorDe Mesa C.L.spa
dc.creatorTorres D.spa
dc.creatorBernal J.spa
dc.creatorGómez A.spa
dc.creatorBriceño I.spa
dc.date.accessioned2020-05-26T00:04:55Z
dc.date.available2020-05-26T00:04:55Z
dc.date.created2010spa
dc.description.abstractIntroduction: Ancient DNA (aDNA)studies can support hypotheses regarding ancient populations; molecular studies can analyze the local population's genetic composition, minimizing biases introduced by later migrations, demographic expansions, mutations, and bottleneck effects. These analyses must be performed with special care because of the low DNA concentrations and contamination risk; therefore, it is necessary to establish protocols to guarantee the reproducibility and veracity of results. Objective: The present study aims to establish a protocol to obtain ancient DNA from 16 pre-Columbian bone samples found in an excavation performed in the area «Candelaria La Nueva» in Bogota, Colombia, dated in the period «Muisca Tardio». Methods: Four founder mitochondrial DNA Amerindian haplotypes were analyzed by high resolution restriction enzyme analyses, obtaining fragments between 121 and 186 base pairs (bp). Different analyses were performed following a strict control of authenticity criteria regarding laboratory conditions, including: positive and negative controls, reproducibility of results, and verification of particular characteristics present in ancient DNA. Results: Results obtained from the bone samples showed the exclusive presence of haplogroup A in the population studied. This data support the statement of the archaeologists of a single biological population in space and time. The distribution of this haplogroup in a 100% frequency supports the hypothesis of Chibcha genetic affiliation. Conclusion: The present study is a contribution to the study of genetic diversity in archaic American populations, allowing the integration of geographic and historic data with genetic characterization techniques associated with linguistic, ethnographic, and glottochronology patterns. Following the protocol proposed in the present study allows fulfilling authenticity criteria for ancient samples with the available techniques. © 2010 Universidad del Valle, Facultad de Salud.eng
dc.format.mimetypeapplication/pdf
dc.identifier.issn16579534
dc.identifier.issn01208322
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23732
dc.language.isoengspa
dc.relation.citationEndPage314
dc.relation.citationIssueNo. 4
dc.relation.citationStartPage306
dc.relation.citationTitleColombia Medica
dc.relation.citationVolumeVol. 41
dc.relation.ispartofColombia Medica, ISSN:16579534, 01208322, Vol.41, No.4 (2010); pp. 306-314spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-78650756373&partnerID=40&md5=97ddce798aedb525522f9b0f1b80bf75spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordMitochondrial DNAspa
dc.subject.keywordAmerican Indianspa
dc.subject.keywordAntiquityspa
dc.subject.keywordArcheologyspa
dc.subject.keywordArticlespa
dc.subject.keywordBase pairingspa
dc.subject.keywordBone agespa
dc.subject.keywordColombiaspa
dc.subject.keywordControlled studyspa
dc.subject.keywordDNA determinationspa
dc.subject.keywordEthnographyspa
dc.subject.keywordGenetic variabilityspa
dc.subject.keywordGeographic distributionspa
dc.subject.keywordHaplotypespa
dc.subject.keywordHumanspa
dc.subject.keywordHuman experimentspa
dc.subject.keywordPopulation biologyspa
dc.subject.keywordRestriction mappingspa
dc.subject.keywordSamplespa
dc.subject.keywordArchaic DNAspa
dc.subject.keywordChibchaspa
dc.subject.keywordMitochondrial DNAspa
dc.subject.keywordMuiscaspa
dc.titleAplication of authenticity criteria in mitochondrial studies on archaic bone remains from a prehispanic Muisca populationspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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