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Multi-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybrids

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dc.creatorFiracative Ropero, Sandra Carolinaspa
dc.date.accessioned2024-01-31T18:31:09Z
dc.date.available2024-01-31T18:31:09Z
dc.date.created2023-12-19spa
dc.date.issued2023spa
dc.descriptionHybrid AD strains of the human pathogenic Cryptococcus neoformans species complex have been reported from many parts of the world. However, their origin, diversity, and evolution are incompletely understood. In this study, we analyzed 102 AD hybrid strains representing 21 countries on five continents. For each strain, we obtained its mating type and its allelic sequences at each of the seven loci that have been used for genotyping haploid serotypes A and D strains of the species complex by the Cryptococcus research community. Our results showed that most AD hybrids exhibited loss of heterozygosity at one or more of the seven analyzed loci. Phylogenetic and population genetic analyses of the allelic sequences revealed multiple origins of the hybrids within each continent, dating back to one million years ago in Africa and up to the present in other continents. We found evidence for clonal reproduction and long-distance dispersal of these hybrids in nature. Comparisons with the global haploid serotypes A and D strains identified new alleles and new haploid multi-locus genotypes in AD hybrids, consistent with the presence of yet-to-be discovered genetic diversity in haploid populations of this species complex in nature. Together, our results indicate that AD hybrids can be effectively genotyped using the same multi-locus sequencing type approach as that established for serotypes A and D strains. Our comparisons of the AD hybrids among each other as well as with the global haploid serotypes A and D strains revealed novel genetic diversity as well as evidence for multiple origins and dynamic evolution of these hybrids in nature.spa
dc.format.mimetypeapplication/pdfspa
dc.identifier.doihttp://doi.org/10.1016/j.fgb.2023.103861spa
dc.identifier.issn1087-1845spa
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/42150
dc.language.isoengspa
dc.publisherUniversidad del Rosariospa
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S1087184523000920/pdfft?md5=95f92c11deffa51bb10ea69034028776&pid=1-s2.0-S1087184523000920-main.pdfspa
dc.rightsAttribution 4.0 Internationalspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccessspa
dc.rights.accesoAbierto (Texto Completo)spa
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/spa
dc.sourceFungal Genetics and Biologyspa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subjectM. Cogliatispa
dc.subjectP.E. Chidebeluspa
dc.subjectM. Hitchcockspa
dc.subjectM. Chenspa
dc.subjectV. Rickertsspa
dc.subjectS. Ackermannspa
dc.subjectM. Desnos Ollivierspa
dc.subjectJ. Ináciospa
dc.subjectU. Nawrotspa
dc.subjectM. Florekspa
dc.subjectK.J. Kwon-Chung jspa
dc.subjectD.-H. Yang jspa
dc.subjectC. Firacativespa
dc.subjectC.A. Puimespa
dc.subjectP. Escandonspa
dc.subjectS. Bertoutspa
dc.subjectF. Rogerspa
dc.subjectJ. Xuspa
dc.titleMulti-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybridsspa
dc.typearticlespa
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersionspa
dc.type.spaArtículospa
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