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16/6-idiotype expressing antibodies induce brain inflammation and cognitive impairment in mice: the mosaic of central nervous system involvement in lupus

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dc.audienceComunidad Rosaristaspa
dc.creatorShaye, Kivity
dc.creatorKatzav, Aviva
dc.creatorArango, María-Teresa
dc.creatorLandau-Rabi, Moran
dc.creatorZafrir, Yaron
dc.creatorAgmon-Levin, Nancy
dc.creatorBlank, Miri
dc.creatorAnaya, Juan-Manuel
dc.creatorMozes, Edna
dc.creatorChapman, Joab
dc.creatorShoenfeld, Yehuda
dc.creator.googleKivity, Shaye
dc.creator.googleKatzav, Aviva
dc.creator.googleArango, Maria Teresa
dc.creator.googleLandau-Rabi, Moran
dc.creator.googleZafrir, Yaron
dc.creator.googleAgmon-Levin, Nancy
dc.creator.googleBlank, Miri
dc.creator.googleAnaya, Juan-Manuel
dc.creator.googleMozes, Edna
dc.creator.googleChapman, Joab
dc.creator.googleShoenfeld, Yehuda
dc.date.accessioned2014-08-13T16:32:59Z
dc.date.available2014-08-13T16:32:59Z
dc.date.created2013-04-04
dc.date.issued2013
dc.description.abstractBackground: The 16/6-idiotype (16/6-Id) of the human anti-DNA antibody was found to induce experimental lupus in naive mice, manifested by production of autoantibodies, leukopenia and elevated inflammatory markers, as well as kidney and brain involvement. We assessed behavior and brain pathology of naive mice injected intracerebra-ventricularly (ICV) with the 16/6-Id antibody. Methods: C3H female mice were injected ICV to the right hemisphere with the human 16/6-Id antibody or commercial human IgG antibodies (control). The mice were tested for depression by the forced swimming test (FST), locomotor and explorative activity by the staircase test, and cognitive functions were examined by the novel object recognition and Y-maze tests. Brain slices were stained for inflammatory processes. Results: 16/6-Id injected mice were cognitively impaired as shown by significant differences in the preference for a new object in the novel object recognition test compared to controls (P = 0.012). Similarly, the preference for spatial novelty in the Y-maze test was significantly higher in the control group compared to the 16/6-Id-injected mice (42% vs. 9%, respectively, P = 0.065). Depression-like behavior and locomotor activity were not significantly different between the16/6-Id-injected and the control mice. Immunohistochemistry analysis revealed an increase in astrocytes and microglial activation in the hippocampus and amygdala, in the 16/6-Id injected group compared to the control. Conclusions: Passive transfer of 16/6-Id antibodies directly into mice brain resulted in cognitive impairments and histological evidence for brain inflammation. These findings shed additional light on the diverse mosaic pathophysiology of neuropsychiatric lupus.eng
dc.format.mediumRecurso electrónicospa
dc.format.mimetypeapplication/pdf
dc.format.tipoDocumentospa
dc.identifier.issnISSN:1741-7015
dc.identifier.urihttp://repository.urosario.edu.co/handle/10336/8833
dc.language.isoeng
dc.publisherUniversidad del Rosariospa
dc.relation.citationIssueNo. 90
dc.relation.citationTitleBMC MEDICINE
dc.relation.citationVolumeVol. 11
dc.relation.ispartofBMC MEDICINE ISSN: 1741-7015 V. 11 N. 90spa
dc.relation.urihttp://www.biomedcentral.com/1741-7015/11/90
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto completo)spa
dc.rights.licenciaEL AUTOR, manifiesta que la obra objeto de la presente autorización es original y la realizó sin violar o usurpar derechos de autor de terceros, por lo tanto la obra es de exclusiva autoría y tiene la titularidad sobre la misma.spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.ddcEnfermedades
dc.subject.decsAnticuerposspa
dc.subject.decsLupus eritematoso sistémicospa
dc.subject.decsEnfermedades autoinmunesspa
dc.subject.keywordMONOCLONAL ANTI-DNAeng
dc.subject.keywordTISSUE-BOUND IMMUNOGLOBULINSeng
dc.subject.keywordHUMAN-HUMAN HYBRIDOMASeng
dc.subject.keywordP-PROTEINS PENETRATEeng
dc.subject.keywordANTIPHOSPHOLIPID SYNDROMEeng
dc.subject.keywordNEUROPSYCHIATRIC LUPUSeng
dc.subject.keywordTOLEROGENIC PEPTIDEeng
dc.subject.keywordRECOGNITION MEMORYeng
dc.subject.keywordSLE PATIENTSeng
dc.subject.keywordAUTOANTIBODIESeng
dc.title16/6-idiotype expressing antibodies induce brain inflammation and cognitive impairment in mice: the mosaic of central nervous system involvement in lupusspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersion
dc.type.spaArtículospa
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