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Capacity of Histoplasma capsulatum to Survive the Composting Process

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dc.creatorGómez Londoño, Luisa Fernandaspa
dc.creatorPérez León, Laura Carolinaspa
dc.creatorMcEwen Ochoa, Juan Guillermospa
dc.creatorZuluaga Rodriguez, Alejandraspa
dc.creatorPeláez Jaramillo, Carlos Albertospa
dc.creatorAcevedo Ruiz, Jose Miguelspa
dc.creatorTaylor, María Luciaspa
dc.creatorArango Arteaga, Myrthaspa
dc.creatorJiménez Alzate, María del Pilarspa
dc.date.accessioned2020-05-25T23:57:02Z
dc.date.available2020-05-25T23:57:02Z
dc.date.created2019spa
dc.description.abstractHistoplasma capsulatum (H. capsulatum) is a thermal-dimorphic fungus, the causal agent of histoplasmosis. Its presence in the environment is related with chicken manure due to their high nitrogen and phosphorus content. In Colombia, chicken manure is the most used raw material in the composting process; however, there is no information about the capacity of H. capsulatum to survive and remain viable in a composted organic fertilizer. To address this question, this study shows three assays based on microbiological culture and the Hc100 nested PCR. First, a composting reactor system was designed to transform organic material under laboratory conditions, and the raw material was inoculated with the fungus. From these reactors, the fungus was not isolated, but its DNA was detected. In the second assay, samples from factories where the DNA of the fungus was previously detected by PCR were analyzed. In the raw material samples, 3 colonies of H. capsulatum were isolated and its DNA was detected. However, after the composting process, neither the fungus was recovered by culture nor DNA was detected. In the third assay, sterilized and nonsterilized organic composted samples were inoculated with H. capsulatum and then evaluated monthly during a year. In both types of samples, the fungus DNA was detected by Hc100 nested PCR during the whole year, but the fungus only grew from sterile samples during the first two months evaluated. In general, the results of the assays showed that H. capsulatum is not able to survive a well-done composting process. © 2019 Luisa Fernanda Gómez Londoño et al.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1155/2019/5038153
dc.identifier.issn16877667
dc.identifier.issn16877675
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22589
dc.language.isoengspa
dc.publisherHindawi Limitedspa
dc.relation.citationTitleApplied and Environmental Soil Science
dc.relation.citationVolumeVol. 2019
dc.relation.ispartofApplied and Environmental Soil Science, ISSN:16877667, 16877675, Vol.2019,(2019)spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85081666589&doi=10.1155%2f2019%2f5038153&partnerID=40&md5=fd17631927fc4161a5ca28f50ed4f848spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordCapacityspa
dc.subject.keywordHistoplasmaspa
dc.subject.keywordcapsulatumspa
dc.subject.keywordSurvivespa
dc.subject.keywordCompostingspa
dc.subject.keywordProcessspa
dc.titleCapacity of Histoplasma capsulatum to Survive the Composting Processspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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