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Characterizing the Mycobacterium tuberculosis Rv2707 protein and determining its sequences which specifically bind to two human cell lines

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dc.creatorChapeton?Montes, Julie A.spa
dc.creatorPlaza, David F.spa
dc.creatorCurtidor, Hernandospa
dc.creatorForero, Marthaspa
dc.creatorVanegas, Magnoliaspa
dc.creatorPatarroyo, Manuel E.spa
dc.creatorPatarroyo, Manuel A.spa
dc.date.accessioned2020-05-25T23:58:36Z
dc.date.available2020-05-25T23:58:36Z
dc.date.created2008spa
dc.description.abstractThe Rv2707 gene encoding a putative alanine- and leucine-rich protein was found to be present in all Mycobacterium tuberculosis complex strains (by PCR) and its transcription was shown by RT-PCR in all but M. bovis and M. microti. Antibodies raised against Rv2707 peptides specifically recognized the native protein by Western blot and were able to locate this protein on the M. tuberculosis membrane by immunoelectron microscopy. A549 and U937 cells lines were used in binding assays involving synthetic peptides covering the whole Rv2707 protein. High A549 cell-binding peptide 16083 ( 281QEEWPAPATHAHRLGNWLKAY300) was identified. Peptides 16072 (61LFGPDTLPAIEKSALSTAHSY80) and 16084 ( 301RIGVGTTTYSSTAQHSAVAA320) presented high specific binding to both A549 and U937 cells. Cross-linking assays revealed that peptide 16084 specifically bound to a 40-kDa and a 50-kDa U937 cell membrane protein. High activity binding peptides (HABPs) 16083 and 16084 were able to inhibit M. tuberculosis invasion of A549 cells. Our results suggest that these sequences could be part of the binding sites used by the bacillus for interacting with target cells, and thus represent good candidates to be tested in a future subunit-based, multiepitope, antituberculosis vaccine. Published by Cold Spring Harbor Laboratory Press. Copyright © 2008 The Protein Society.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1110/ps.073083308
dc.identifier.issn9618368
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22893
dc.language.isoengspa
dc.relation.citationEndPage351
dc.relation.citationIssueNo. 2
dc.relation.citationStartPage342
dc.relation.citationTitleProtein Science
dc.relation.citationVolumeVol. 17
dc.relation.ispartofProtein Science, ISSN:9618368, Vol.17, No.2 (2008); pp. 342-351spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-38649085597&doi=10.1110%2fps.073083308&partnerID=40&md5=cbe73a4fab2ccb62d7cf0f2fd23f15d5spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordBacterial proteinspa
dc.subject.keywordProtein rv2707spa
dc.subject.keywordUnclassified drugspa
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordArticlespa
dc.subject.keywordControlled studyspa
dc.subject.keywordGene locationspa
dc.subject.keywordGene sequencespa
dc.subject.keywordHumanspa
dc.subject.keywordHuman cellspa
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordNonhumanspa
dc.subject.keywordPriority journalspa
dc.subject.keywordReverse transcription polymerase chain reactionspa
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordBacterial proteinsspa
dc.subject.keywordCell lineeng
dc.subject.keywordComputational biologyspa
dc.subject.keywordHumansspa
dc.subject.keywordMicroscopyeng
dc.subject.keywordMolecular sequence dataspa
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordPeptide fragmentsspa
dc.subject.keywordProtein bindingspa
dc.subject.keywordU937 cellsspa
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordMycobacterium tuberculosis complexspa
dc.subject.keywordA549 cellspa
dc.subject.keywordHigh activity binding peptide (habp)spa
dc.subject.keywordInvasionspa
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordRv2707spa
dc.subject.keywordU937 cellspa
dc.titleCharacterizing the Mycobacterium tuberculosis Rv2707 protein and determining its sequences which specifically bind to two human cell linesspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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