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CagA gene EPIYA motif genetic characterization from Colombian Helicobacter pylori isolates: Standardization of a molecular test for rapid clinical laboratory detection

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dc.creatorGómez, Eliana Rocío Rodríguezspa
dc.creatorRegino, William Oterospa
dc.creatorMonterrey, Pedro A.spa
dc.creatorRangel, Alba Alicia Trespalaciosspa
dc.date.accessioned2020-05-25T23:58:19Z
dc.date.available2020-05-25T23:58:19Z
dc.date.created2020spa
dc.description.abstractThe aim of this work was to determine current cagA gene EPIYA motifs present in Colombian Helicobacter pylori isolates using a fast and reliable molecular test. DNA from eightyfive Helicobacter pylori-cagA positive strains were analyzed. Strains were obtained from patients diagnosed with functional dyspepsia at Cl? nica Fundadores in Bogota . The 3' region of the cagA gene was amplified through conventional Polymerase Chain Reaction (PCR). Obtained amplicons were sequenced using the Sanger method and analyzed with bioinformatics tools. Additionally, a significant Spearman correlation coefficient was determined between the patients' age and the number of EPIYA-C repeats; with p values less than 0.05 considered significant. Estimates were obtained using a 95% CI. The 3 variable region of the cagA gene was amplified and PCR products of the following sizes corresponded to the following EPIYA motifs: 400 bp: EPIYA AB, 500 bp: EPIYA ABC, 600 bp: EPIYA ABCC and 700 bp: ABCCC. A single PCR band was observed for 58 out of 85 Helicobacter pylori isolates, with an EPIYA distribution motif as follows: 7/85 AB (8.2%), 34/85 ABC (40%), 26/85 ABCC (30.6%) and 18/85 ABCCC (21.2%). However, in 27 out of 85 Helicobacter pylori isolates, two or more bands were observed, where the most predominant cagA genotype were ABC-ABCC (26%, 7/27) and ABCC-ABCCC (22.2%, 6/27). A direct proportionality between the number of EPIYA-C repeats and an increase in the patients' age was observed, finding a greater number of EPIYA ABCC and ABCCC repeats in the population over 50 years old. All isolates were of the Western cagA type and 51.8% of them were found to have multiple EPIYA-C repeats. These standardized molecular test allowed to identify the number of EPIYA C motifs based on band size. © 2020 Rodriguez Gomez et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0227275
dc.identifier.issn19326203
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22838
dc.language.isoengspa
dc.publisherPublic Library of Sciencespa
dc.relation.citationIssueNo. 1
dc.relation.citationTitlePLoS ONE
dc.relation.citationVolumeVol. 15
dc.relation.ispartofPLoS ONE, ISSN:19326203, Vol.15, No.1 (2020)spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85077765342&doi=10.1371%2fjournal.pone.0227275&partnerID=40&md5=b8b97d5292246b5a362e7427d5a75e25spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordCaga proteinspa
dc.subject.keywordAdultspa
dc.subject.keywordAgespa
dc.subject.keywordAgedspa
dc.subject.keywordAmpliconspa
dc.subject.keywordArticlespa
dc.subject.keywordBacterial genespa
dc.subject.keywordBacterial strainspa
dc.subject.keywordBacterium detectionspa
dc.subject.keywordBacterium isolatespa
dc.subject.keywordBinding affinityspa
dc.subject.keywordBioinformaticsspa
dc.subject.keywordCaga genespa
dc.subject.keywordCancer riskspa
dc.subject.keywordColombianspa
dc.subject.keywordDiagnostic accuracyspa
dc.subject.keywordDyspepsiaspa
dc.subject.keywordGene amplificationspa
dc.subject.keywordGenetic analysisspa
dc.subject.keywordGenetic variabilityspa
dc.subject.keywordGenotypespa
dc.subject.keywordHelicobacter pylorispa
dc.subject.keywordHigh risk patientspa
dc.subject.keywordHumanspa
dc.subject.keywordInfection controlspa
dc.subject.keywordMiddle agedspa
dc.subject.keywordMolecular biologyspa
dc.subject.keywordMolecular geneticsspa
dc.subject.keywordMolecular weightspa
dc.subject.keywordNonhumanspa
dc.subject.keywordPhenotypespa
dc.subject.keywordPolymerase chain reactionspa
dc.subject.keywordProtein motifspa
dc.subject.keywordRisk reductionspa
dc.subject.keywordSanger sequencingspa
dc.subject.keywordSequence alignmentspa
dc.subject.keywordStandardizationspa
dc.subject.keywordStomach cancerspa
dc.titleCagA gene EPIYA motif genetic characterization from Colombian Helicobacter pylori isolates: Standardization of a molecular test for rapid clinical laboratory detectionspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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