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Striking divergence in toxoplasma ROP16 nucleotide sequences from human and meat samples

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dc.creatorAlvarez, Catalinaspa
dc.creatorde-la-Torre, Alejandraspa
dc.creatorVargas, Mónicaspa
dc.creatorHerrera, Claudiaspa
dc.creatorUribe-Huertas, Lored Dayanaspa
dc.creatorLora, Fabianaspa
dc.creatorGómez-Marín, Jorge Enriquespa
dc.date.accessioned2020-05-25T23:56:16Z
dc.date.available2020-05-25T23:56:16Z
dc.date.created2015spa
dc.description.abstractBackground. ROP16 is a protein kinase of Toxoplasma gondii identified in the mouse model as a virulent marker, but it is unknown whether this finding is relevant in human toxoplasmosis. Methods. We obtained the Toxoplasma ROP16 locus DNA sequence in samples from 12 patients with ocular toxoplasmosis, 1 sample from a patient with congenital toxoplasmosis, 22 samples from soldiers operating in the jungle, 2 samples from urban soldiers, and 10 samples from meat for human consumption. An enzyme-linked immunosorbent assay specific for antibodies against the ROP16 mouse-virulent peptide was performed in 46 serum specimens from patients with ocular toxoplasmosis and in 28 serum specimens from patients with chronic asymptomatic infection, of whom 19 had congenital infection and 11 had toxoplasmic lymphadenitis. Results. We found a striking divergence of the ROP16 nucleotide sequences. Ten of 12 sequences (83.3%) from patients with ocular toxoplasmosis clustered with those of mouse-virulent strains, whereas 7 of 7 ROP16 sequences (100%) from meat were clustered with those of mouse-avirulent strains. Only 11 of 104 serum specimens (10.5%) had specific antibodies against the mouse-virulent peptide, and there was no association between clinical forms and positive results of serological assays. Conclusions. The majority of ROP16 nucleotide sequences from Colombian patients with ocular toxoplasmosis belonged to the group of mouse-virulent strains. © 2014 The Author.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1093/infdis/jiu833
dc.identifier.issn00221899
dc.identifier.issn15376613
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22377
dc.language.isoengspa
dc.publisherOxford University Pressspa
dc.relation.citationEndPage2013
dc.relation.citationIssueNo. 12
dc.relation.citationStartPage2006
dc.relation.citationTitleJournal of Infectious Diseases
dc.relation.citationVolumeVol. 211
dc.relation.ispartofJournal of Infectious Diseases, ISSN:00221899, 15376613, Vol.211, No.12 (2015); pp. 2006-2013spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84930372539&doi=10.1093%2finfdis%2fjiu833&partnerID=40&md5=5a50a0a8a5b0957962d715d20cd4664aspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordProtein kinasespa
dc.subject.keywordtoxoplasma gondiieng
dc.subject.keywordRop16 proteinspa
dc.subject.keyworddnaeng
dc.subject.keywordUnclassified drugspa
dc.subject.keywordProtein tyrosine kinasespa
dc.subject.keywordProtozoal proteinspa
dc.subject.keywordRop16 proteineng
dc.subject.keywordArticlespa
dc.subject.keywordBeef cattlespa
dc.subject.keywordChicken meatspa
dc.subject.keywordChildspa
dc.subject.keywordCluster analysisspa
dc.subject.keywordColombianspa
dc.subject.keywordDna sequencespa
dc.subject.keywordEnzyme linked immunosorbent assayspa
dc.subject.keywordGene locusspa
dc.subject.keywordGenetic strainspa
dc.subject.keywordGenetic variabilityspa
dc.subject.keywordHost rangespa
dc.subject.keywordHumanspa
dc.subject.keywordMajor clinical studyspa
dc.subject.keywordNonhumanspa
dc.subject.keywordParasite virulencespa
dc.subject.keywordPhylogenyspa
dc.subject.keywordPolymerase chain reactionspa
dc.subject.keywordPorkspa
dc.subject.keywordPriority journalspa
dc.subject.keywordSerotypingspa
dc.subject.keywordSoldierspa
dc.subject.keywordToxoplasma gondiispa
dc.subject.keywordColombiaspa
dc.subject.keywordDna sequencespa
dc.subject.keywordFemalespa
dc.subject.keywordGeneticsspa
dc.subject.keywordGenotypespa
dc.subject.keywordIsolation and purificationspa
dc.subject.keywordMalespa
dc.subject.keywordMeatspa
dc.subject.keywordParasitologyspa
dc.subject.keywordPregnancyspa
dc.subject.keywordToxoplasmaspa
dc.subject.keywordToxoplasmosisspa
dc.subject.keywordChildspa
dc.subject.keywordColombiaspa
dc.subject.keywordFemalespa
dc.subject.keywordGenetic variationspa
dc.subject.keywordGenotypespa
dc.subject.keywordHumansspa
dc.subject.keywordMalespa
dc.subject.keywordMeatspa
dc.subject.keywordPregnancyspa
dc.subject.keywordProtein-tyrosine kinasesspa
dc.subject.keywordProtozoan proteinsspa
dc.subject.keywordSequence analysiseng
dc.subject.keywordToxoplasmaspa
dc.subject.keywordToxoplasmosisspa
dc.subject.keywordFoodborne diseasesspa
dc.subject.keywordInfectious uveitisspa
dc.subject.keywordRop16 proteinspa
dc.subject.keywordToxoplasmaspa
dc.subject.keywordVirulencespa
dc.titleStriking divergence in toxoplasma ROP16 nucleotide sequences from human and meat samplesspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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