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Proteolytic hydrolysis and purification of the LRP/alfa-2-macroglobulin receptor domain from ?-macroglobulins

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dc.creatorBarrera, Daniel Ivánspa
dc.creatorMatheus Merino, Luisa Marina
dc.creatorStigbrand, Torgnyspa
dc.creatorArbeláez, Luis Fernandospa
dc.date.accessioned2020-05-25T23:58:35Z
dc.date.available2020-05-25T23:58:35Z
dc.date.created2007spa
dc.description.abstractA new, easier and efficient purification method, using Sephacryl and DEAE-Sephacel, of the C-terminal fragment of two ?-macroglobulins, ?2-M and PZP, is presented. Two larger peptides were identified for each protein as the C-terminal fragment, with molecular weights of ?30 kDa and the N-terminal sequences were determined to be SSTQDTV for ?2-M and VALHLS for PZP. The smaller peptides with molecular weights of 18 kDa correspond to a shorter C-terminal sequence of these proteins, and they were determined to be EEFPFA for ?2-M and ALKVQTV for PZP, with no interfering sequences detected. The results confirmed the discriminatory capacity of the purification procedure and the purity of the fragments. This new methodology facilitates biological studies of ?-macroglobulins, and will enable elucidation of the role the C-terminal region may exert to eliminate ?-macroglobulin-proteinases complexes from the circulation by the LRP/receptor. © 2006 Elsevier Inc. All rights reserved.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.pep.2006.12.008
dc.identifier.issn10960279
dc.identifier.issn10465928
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22891
dc.language.isoengspa
dc.relation.citationEndPage118
dc.relation.citationIssueNo. 1
dc.relation.citationStartPage112
dc.relation.citationTitleProtein Expression and Purification
dc.relation.citationVolumeVol. 53
dc.relation.ispartofProtein Expression and Purification, ISSN:10960279, 10465928, Vol.53, No.1 (2007); pp. 112-118spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-33847163955&doi=10.1016%2fj.pep.2006.12.008&partnerID=40&md5=9730f35888b3b2710a8328fcb7d80894spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordAlpha 2 macroglobulinspa
dc.subject.keywordhumaneng
dc.subject.keywordLow density lipoprotein receptor related proteinspa
dc.subject.keywordMonoclonal antibodyspa
dc.subject.keywordPeptide fragmentspa
dc.subject.keywordPlacenta proteinspa
dc.subject.keywordProteinasespa
dc.subject.keywordPzp proteineng
dc.subject.keywordUnclassified drugspa
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordArticlespa
dc.subject.keywordChemistryspa
dc.subject.keywordEnzyme linked immunosorbent assayspa
dc.subject.keywordFemalespa
dc.subject.keywordHumanspa
dc.subject.keywordHydrolysisspa
dc.subject.keywordIsolation and purificationspa
dc.subject.keywordMetabolismspa
dc.subject.keywordMolecular geneticsspa
dc.subject.keywordMolecular weightspa
dc.subject.keywordPolyacrylamide gel electrophoresisspa
dc.subject.keywordPregnancyspa
dc.subject.keywordProtein tertiary structurespa
dc.subject.keywordSequence analysisspa
dc.subject.keywordTimespa
dc.subject.keywordWestern blottingspa
dc.subject.keywordAlpha-macroglobulinsspa
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordAntibodieseng
dc.subject.keywordBlottingeng
dc.subject.keywordChymotrypsinspa
dc.subject.keywordElectrophoresiseng
dc.subject.keywordEndopeptidasesspa
dc.subject.keywordEnzyme-linked immunosorbent assayspa
dc.subject.keywordFemalespa
dc.subject.keywordHumansspa
dc.subject.keywordHydrolysisspa
dc.subject.keywordLdl-receptor related protein 1spa
dc.subject.keywordMolecular sequence dataspa
dc.subject.keywordMolecular weightspa
dc.subject.keywordPeptide fragmentsspa
dc.subject.keywordPregnancyspa
dc.subject.keywordPregnancy proteinsspa
dc.subject.keywordProtein structureeng
dc.subject.keywordSequence analysiseng
dc.subject.keywordTime factorsspa
dc.subject.keyword?-macroglobulinsspa
dc.subject.keywordC-terminal regionspa
dc.subject.keywordChymotrypsinspa
dc.subject.keywordPzpspa
dc.titleProteolytic hydrolysis and purification of the LRP/alfa-2-macroglobulin receptor domain from ?-macroglobulinsspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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