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Hypoxia-inducible factor HIF-1? modulates drugs resistance in colon cancer cells

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dc.creatorPinzón-Daza M.L.spa
dc.creatorCuellar Y.spa
dc.creatorOndo Méndez, Alejandro Oyonospa
dc.creatorMatheus Merino, Luisa Marinaspa
dc.creatorDel Riesgo Prendes, Liliaspa
dc.creatorCastillo F.spa
dc.creatorGarzón R.spa
dc.date.accessioned2020-05-26T00:00:18Z
dc.date.available2020-05-26T00:00:18Z
dc.date.created2018spa
dc.description.abstractIntroduction: Drug resistance mechanisms may be associated with decreased cell death and its induction may depend on the response to oxidative stress caused by hypoxia. The correlation between hypoxia-inducible factor HIF-1?, the number of reactive oxygen species and their effect on cell survival has not yet been evaluated. Objective: The purpose of this study was to evaluate the effect of HIF-1? activity and reactive oxygen species (ROS) accumulation in apoptosis of colon cancer cells. Materials and methods: HT29 colon cancer cells were treated with Cobalt(II) chloride (CoCl2) or doxorubicin and the activity of HIF-1? was determined by ELISA assay. ROS were determined using fluorescence probe carboxy-H2DFFDA. Apoptosis was assessed by caspase-3 activation analysis, and PUMA and BAX mRNA levels by qRT-PCR. The reduction of the antiapoptotic effect due to hypoxia was attenuated by use of the endonuclease APE-1 (E3330) inhibitor. The endonuclease E3330 APE-1 inhibitor allowed evaluating the effect of ROS generated by doxorubicin and CoCl2 on apoptosis. Results: Chemical hypoxia in combination with doxorubicin is an oxidative stressor in HT29 cells and induces a reduction in the apoptotic process in a time-dependent manner. Conclusion: Resistance to hypoxia and doxorubicin-mediated cell death could be controlled by a mechanism related to the activity of HIF-1? and the amount of reactive oxygen species generated. © 2018, Universidad Nacional de Colombia. All rights reserved.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.15446/revfacmed.v66n4.55149
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23196
dc.language.isoengspa
dc.publisherUniversidad Nacional de Colombiaspa
dc.relation.citationEndPage550
dc.relation.citationIssueNo. 4
dc.relation.citationStartPage543
dc.relation.citationTitleRevista Facultad de Medicina
dc.relation.citationVolumeVol. 66
dc.relation.ispartofRevista Facultad de Medicina, Vol.66, No.4 (2018); pp. 543-550spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85062694984&doi=10.15446%2frevfacmed.v66n4.55149&partnerID=40&md5=3977b44f9d88b4fbe828dd11282928f6spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordApoptosisspa
dc.subject.keywordCell hypoxiaspa
dc.subject.keywordColon cancerspa
dc.subject.keywordDoxorubicin (mesh)spa
dc.titleHypoxia-inducible factor HIF-1? modulates drugs resistance in colon cancer cellsspa
dc.title.TranslatedTitleFactor inducible por hipoxia HIF-1? modula la resistencia a drogas en células de cáncer de colonspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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