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Th1 and Th2 immune response to P30 and ROP18 peptides in human toxoplasmosis

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dc.creatorTorres-Morales, Elizabethspa
dc.creatorTaborda, Lauraspa
dc.creatorCardona, Nestorspa
dc.creatorde-la-Torre, Alejandraspa
dc.creatorSepulveda-Arias, Juan Carlosspa
dc.creatorPatarroyo, Manuel A.
dc.creatorGomez-Marin, Jorge Enriquespa
dc.date.accessioned2020-05-25T23:56:57Z
dc.date.available2020-05-25T23:56:57Z
dc.date.created2014spa
dc.description.abstractWe determined the specific lymphocyte proliferative response and cytokine profile production regarding Toxoplasma P30 (2017 from virulent and non-virulent strain) and ROP18 protein-derived peptides (from clonal lineages I, II and III) in 19 patients having ocular toxoplasmosis, five suffering chronic asymptomatic infection, nine with congenital toxoplasmosis and eight Toxoplasma negative people. A Beckman Coulter FC500 flow cytometer was used for determining antigen-specific T cells (CD3+ CD4+ or CD3+ CD8+ cells) in peripheral blood culture. IFN ? and IL10 levels were determined in culture supernatants. Specific CD4+ and CD8+ T cell response to total antigen and P30- and ROP18-derived peptides was observed in infected people. Ocular toxoplasmosis patients had a preferential Th2 response after antigenic stimulation. Non-virulent peptide 2017 was able to shift response toward Th1 in congenitally infected children and virulent peptide 2017 induced a Th2 response in chronically infected, asymptomatic people. An immune response in human toxoplasmosis after ex vivo antigenic stimulation was Th1- or Th2-skewed, depending on a patient’s clinical condition. Colombian ocular toxoplasmosis patients’ immune response was Th2-skewed, regardless of the nature of antigen stimulus. © 2014, Springer-Verlag Berlin Heidelberg.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1007/s00430-014-0339-0
dc.identifier.issn14321831
dc.identifier.issn03008584
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22571
dc.language.isoengspa
dc.publisherSpringer Verlagspa
dc.relation.citationEndPage322
dc.relation.citationIssueNo. 5
dc.relation.citationStartPage315
dc.relation.citationTitleMedical Microbiology and Immunology
dc.relation.citationVolumeVol. 203
dc.relation.ispartofMedical Microbiology and Immunology, ISSN:14321831, 03008584, Vol.203, No.5 (2014); pp. 315-322spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84919477257&doi=10.1007%2fs00430-014-0339-0&partnerID=40&md5=4b6c72c20def77684d4ff6b2fb732f31spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordCD3 antigenspa
dc.subject.keywordCD4 antigenspa
dc.subject.keywordProtozoaneng
dc.subject.keywordToxoplasmaeng
dc.subject.keywordCDeng
dc.subject.keywordMononucleareng
dc.subject.keywordToxoplasma gondiieng
dc.subject.keywordCD8 antigenspa
dc.subject.keywordGamma interferonspa
dc.subject.keywordInterleukin 10spa
dc.subject.keywordProtein p30spa
dc.subject.keywordROP18 proteinspa
dc.subject.keywordTumor necrosis factor alphaspa
dc.subject.keywordUnclassified drugspa
dc.subject.keywordVirulence factorspa
dc.subject.keywordCytokinespa
dc.subject.keywordLeukocyte antigenspa
dc.subject.keywordParasite antigenspa
dc.subject.keywordProtein serine threonine kinasespa
dc.subject.keywordProtozoal proteinspa
dc.subject.keywordROP18 proteineng
dc.subject.keywordSAG1 antigeneng
dc.subject.keywordAdultspa
dc.subject.keywordAntigen specificityspa
dc.subject.keywordArticlespa
dc.subject.keywordAsymptomatic diseasespa
dc.subject.keywordCarboxy terminal sequencespa
dc.subject.keywordCD4+ T lymphocytespa
dc.subject.keywordCD8+ T lymphocytespa
dc.subject.keywordChildspa
dc.subject.keywordClinical articlespa
dc.subject.keywordControlled studyspa
dc.subject.keywordCytokine productionspa
dc.subject.keywordEx vivo studyspa
dc.subject.keywordFemalespa
dc.subject.keywordHumanspa
dc.subject.keywordHuman cellspa
dc.subject.keywordImmune responsespa
dc.subject.keywordImmunostimulationspa
dc.subject.keywordMalespa
dc.subject.keywordNonhumanspa
dc.subject.keywordParasite virulencespa
dc.subject.keywordTh1 cellspa
dc.subject.keywordTh2 cellspa
dc.subject.keywordToxoplasmosisspa
dc.subject.keywordAdolescentspa
dc.subject.keywordCell proliferationspa
dc.subject.keywordChemistryspa
dc.subject.keywordFlow cytometryspa
dc.subject.keywordImmunologyspa
dc.subject.keywordMiddle agedspa
dc.subject.keywordMononuclear cellspa
dc.subject.keywordSecretion (process)spa
dc.subject.keywordT lymphocyte subpopulationspa
dc.subject.keywordTh1 cellspa
dc.subject.keywordTh2 cellspa
dc.subject.keywordToxoplasmosisspa
dc.subject.keywordYoung adultspa
dc.subject.keywordAdolescentspa
dc.subject.keywordAdultspa
dc.subject.keywordAntigenseng
dc.subject.keywordAntigenseng
dc.subject.keywordCD4-Positive T-Lymphocytesspa
dc.subject.keywordCD8-Positive T-Lymphocytesspa
dc.subject.keywordCell Proliferationspa
dc.subject.keywordChildspa
dc.subject.keywordCytokinesspa
dc.subject.keywordFemalespa
dc.subject.keywordFlow Cytometryspa
dc.subject.keywordHumansspa
dc.subject.keywordLeukocyteseng
dc.subject.keywordMalespa
dc.subject.keywordMiddle Agedspa
dc.subject.keywordProtein-Serine-Threonine Kinasesspa
dc.subject.keywordProtozoan Proteinsspa
dc.subject.keywordT-Lymphocyte Subsetsspa
dc.subject.keywordTh1 Cellsspa
dc.subject.keywordTh2 Cellsspa
dc.subject.keywordToxoplasmosisspa
dc.subject.keywordYoung Adultspa
dc.subject.keywordIL10spa
dc.subject.keywordInterferon gammaspa
dc.subject.keywordP30 proteinspa
dc.subject.keywordPeptidesspa
dc.subject.keywordROP18spa
dc.subject.keywordTh1spa
dc.subject.keywordTh2spa
dc.subject.keywordToxoplasmaspa
dc.subject.keywordVaccinespa
dc.titleTh1 and Th2 immune response to P30 and ROP18 peptides in human toxoplasmosisspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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