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The H3K9 methylation writer SETDB1 and its reader MPP8 cooperate to silence satellite DNA repeats in mouse embryonic stem cells

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dc.creatorCruz-Tapias, Paolaspa
dc.creatorRobin, Philippespa
dc.creatorPontis, Julienspa
dc.creatorDel Maestro, Laurencespa
dc.creatorAit-Si-Ali, Slimanespa
dc.date.accessioned2020-05-26T00:09:11Z
dc.date.available2020-05-26T00:09:11Z
dc.date.created2019spa
dc.description.abstractSETDB1 (SET Domain Bifurcated histone lysine methyltransferase 1) is a key lysine methyltransferase (KMT) required in embryonic stem cells (ESCs), where it silences transposable elements and DNA repeats via histone H3 lysine 9 tri-methylation (H3K9me3), independently of DNA methylation. The H3K9 methylation reader M-Phase Phosphoprotein 8 (MPP8) is highly expressed in ESCs and germline cells. Although evidence of a cooperation between H3K9 KMTs and MPP8 in committed cells has emerged, the interplay between H3K9 methylation writers and MPP8 in ESCs remains elusive. Here, we show that MPP8 interacts physically and functionally with SETDB1 in ESCs. Indeed, combining biochemical, transcriptomic and genomic analyses, we found that MPP8 and SETDB1 co-regulate a significant number of common genomic targets, especially the DNA satellite repeats. Together, our data point to a model in which the silencing of a class of repeated sequences in ESCs involves the cooperation between the H3K9 methylation writer SETDB1 and its reader MPP8. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.3390/genes10100750
dc.identifier.issn20734425
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/24143
dc.language.isoengspa
dc.publisherMDPI AGspa
dc.relation.citationIssueNo. 10
dc.relation.citationTitleGenes
dc.relation.citationVolumeVol. 10
dc.relation.ispartofGenes, ISSN:20734425, Vol.10, No.10 (2019)spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85072707703&doi=10.3390%2fgenes10100750&partnerID=40&md5=bf4c358ead2238833eb5b3b662e84a34spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordHistone H3spa
dc.subject.keywordCulturedeng
dc.subject.keywordM phase phosphoprotein 8spa
dc.subject.keywordPhosphoproteinspa
dc.subject.keywordRepetitive DNAspa
dc.subject.keywordSatellite DNAspa
dc.subject.keywordSET domain bifurcated histone lysine methyltransferase 1spa
dc.subject.keywordSmall interfering RNAspa
dc.subject.keywordUnclassified drugspa
dc.subject.keywordHistonespa
dc.subject.keywordHistone lysine methyltransferasespa
dc.subject.keywordMphosph8 proteineng
dc.subject.keywordPhosphoproteinspa
dc.subject.keywordProtein bindingspa
dc.subject.keywordSatellite DNAspa
dc.subject.keywordSETDB1 proteineng
dc.subject.keywordAnimal cellspa
dc.subject.keywordArticlespa
dc.subject.keywordBinding sitespa
dc.subject.keywordBiochemical analysisspa
dc.subject.keywordCell cycle M phasespa
dc.subject.keywordChromatin immunoprecipitationspa
dc.subject.keywordComparative studyspa
dc.subject.keywordComplex formationspa
dc.subject.keywordControlled studyspa
dc.subject.keywordDown regulationspa
dc.subject.keywordEmbryospa
dc.subject.keywordGenespa
dc.subject.keywordGene controlspa
dc.subject.keywordGene expressionspa
dc.subject.keywordGene expression regulationspa
dc.subject.keywordGene functionspa
dc.subject.keywordGene numberspa
dc.subject.keywordGene silencingspa
dc.subject.keywordGene targetingspa
dc.subject.keywordGenomicsspa
dc.subject.keywordHela S3 cell linespa
dc.subject.keywordHistone methylationspa
dc.subject.keywordImmunoprecipitationspa
dc.subject.keywordLong interspersed nuclear elementspa
dc.subject.keywordLoss of function mutationspa
dc.subject.keywordMolecular geneticsspa
dc.subject.keywordMousespa
dc.subject.keywordMouse embryonic stem cellspa
dc.subject.keywordMPP8 genespa
dc.subject.keywordNonhumanspa
dc.subject.keywordProtein processingspa
dc.subject.keywordProtein protein interactionspa
dc.subject.keywordRNA sequencespa
dc.subject.keywordSETDB1 genespa
dc.subject.keywordShort interspersed nuclear elementspa
dc.subject.keywordTranscriptomicsspa
dc.subject.keywordUpregulationspa
dc.subject.keywordAnimalspa
dc.subject.keywordCell culturespa
dc.subject.keywordGeneticsspa
dc.subject.keywordHela cell linespa
dc.subject.keywordHumanspa
dc.subject.keywordMetabolismspa
dc.subject.keywordMouse embryonic stem cellspa
dc.subject.keywordAnimalsspa
dc.subject.keywordCellseng
dc.subject.keywordDNAeng
dc.subject.keywordHela Cellsspa
dc.subject.keywordHistone-Lysine N-Methyltransferasespa
dc.subject.keywordHistonesspa
dc.subject.keywordHumansspa
dc.subject.keywordMicespa
dc.subject.keywordMouse Embryonic Stem Cellsspa
dc.subject.keywordPhosphoproteinsspa
dc.subject.keywordProtein Bindingspa
dc.subject.keywordHistone lysine methylationspa
dc.subject.keywordM-phase phosphoprotein 8spa
dc.subject.keywordSatellite DNA repeatsspa
dc.subject.keywordSETDB1/KMT1Espa
dc.subject.keywordTranscriptionspa
dc.titleThe H3K9 methylation writer SETDB1 and its reader MPP8 cooperate to silence satellite DNA repeats in mouse embryonic stem cellsspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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