Ítem
Solo Metadatos

Regulation of PfEMP1-VAR2CSA translation by a Plasmodium translation-enhancing factor

Mostrar el registro sencillo de la publicación
dc.creatorChan S.spa
dc.creatorFrasch A.spa
dc.creatorMandava C.S.spa
dc.creatorCh'Ng J.-H.spa
dc.creatorQuintana M.D.P.spa
dc.creatorVesterlund M.spa
dc.creatorGhorbal M.spa
dc.creatorJoannin N.spa
dc.creatorFranzén O.spa
dc.creatorLopez-Rubio J.-J.spa
dc.creatorBarbieri S.spa
dc.creatorLanzavecchia A.spa
dc.creatorSanyal S.spa
dc.creatorWahlgren M.spa
dc.date.accessioned2020-05-25T23:57:18Z
dc.date.available2020-05-25T23:57:18Z
dc.date.created2017spa
dc.description.abstractPregnancy-associated malaria commonly involves the binding of Plasmodium falciparum-infected erythrocytes to placental chondroitin sulfate A (CSA) through the PfEMP1-VAR2CSA protein. VAR2CSA is translationally repressed by an upstream open reading frame. In this study, we report that the P. falciparum translation enhancing factor (PTEF) relieves upstream open reading frame repression and thereby facilitates VAR2CSA translation. VAR2CSA protein levels in var2csa-transcribing parasites are dependent on the expression level of PTEF, and the alleviation of upstream open reading frame repression requires the proteolytic processing of PTEF by PfCalpain. Cleavage generates a C-terminal domain that contains a sterile-alpha-motif-like domain. The C-terminal domain is permissive to cytoplasmic shuttling and interacts with ribosomes to facilitate translational derepression of the var2csa coding sequence. It also enhances translation in a heterologous translation system and thus represents the first non-canonical translation enhancing factor to be found in a protozoan. Our results implicate PTEF in regulating placental CSA binding of infected erythrocytes. © 2017 Macmillan Publishers Limited. All rights reserved.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1038/nmicrobiol.2017.68
dc.identifier.issn20585276
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22642
dc.language.isoengspa
dc.publisherNature Publishing Groupspa
dc.relation.citationTitleNature Microbiology
dc.relation.citationVolumeVol. 2
dc.relation.ispartofNature Microbiology, ISSN:20585276, Vol.2,(2017)spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85019133411&doi=10.1038%2fnmicrobiol.2017.68&partnerID=40&md5=615e11cc58ca3cd5cc3038e24ad9c9efspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordCalpainspa
dc.subject.keywordparasiticeng
dc.subject.keywordParasite antigenspa
dc.subject.keywordProtozoal proteinspa
dc.subject.keywordVar2csa proteineng
dc.subject.keywordErythrocytespa
dc.subject.keywordFemalespa
dc.subject.keywordGene expression regulationspa
dc.subject.keywordGeneticsspa
dc.subject.keywordHumanspa
dc.subject.keywordMalaria falciparumspa
dc.subject.keywordMetabolismspa
dc.subject.keywordOpen reading framespa
dc.subject.keywordParasitologyspa
dc.subject.keywordPlacentaspa
dc.subject.keywordPlasmodiumspa
dc.subject.keywordPlasmodium falciparumspa
dc.subject.keywordPregnancyspa
dc.subject.keywordPregnancy complicationspa
dc.subject.keywordProtein degradationspa
dc.subject.keywordProtein synthesisspa
dc.subject.keywordAntigenseng
dc.subject.keywordCalpainspa
dc.subject.keywordChondroitin sulfatesspa
dc.subject.keywordErythrocytesspa
dc.subject.keywordFemalespa
dc.subject.keywordGene expression regulationspa
dc.subject.keywordHumansspa
dc.subject.keywordMalariaeng
dc.subject.keywordOpen reading framesspa
dc.subject.keywordPlacentaspa
dc.subject.keywordPlasmodiumspa
dc.subject.keywordPlasmodium falciparumspa
dc.subject.keywordPregnancyspa
dc.subject.keywordPregnancy complicationseng
dc.subject.keywordProtein biosynthesisspa
dc.subject.keywordProteolysisspa
dc.subject.keywordProtozoan proteinsspa
dc.titleRegulation of PfEMP1-VAR2CSA translation by a Plasmodium translation-enhancing factorspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
Archivos
Colecciones
Artículos