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Dissecting the Heterogeneous Population Genetic Structure of Candida albicans: Limitations and constraints of the multilocus sequence typing scheme

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dc.creatorMuñoz, Marinaspa
dc.creatorWintaco, Luz Mairaspa
dc.creatorMuñoz, Shirly Alexandraspa
dc.creatorRamírez, Juan David
dc.date.accessioned2020-05-26T00:08:33Z
dc.date.available2020-05-26T00:08:33Z
dc.date.created2019spa
dc.description.abstractCandida albicans is a fungal opportunistic pathogen of significant public health importance mainly due to the recent emergence of strains with increased aggressiveness and antifungal resistance. Here, we aimed to describe the epidemiological profiles and approximate the population structure of C. albicans by analyzing the C. albicans multilocus sequence typing (MLST) database (Calb-MLST-DB), which contains the largest publically available dataset for this species. Based on 4,318 database isolates, we confirmed the ubiquitous nature of C. albicans including a group of diploid sequence types (DSTs) obtained from Healthy individuals exclusively (taken as an indicator of lack of association with illnesses in its host), until isolates established from Non-Healthy individuals (potentially associated with pathogenic processes) and other DSTs reported in both types (Healthy and Non-Healthy). The highest number of reported DSTs was related to blood, oral and vaginal swabs (32.4, 20.5, and 13.8%, respectively). High genetic diversity was observed in the seven housekeeping genes included in the MLST scheme, with a diverse population structure (154 clonal complexes, CCs; and a high number of singletons, n = 1,074). Phylogenetic reconstruction on the concatenated alignment of these housekeeping genes for all the reported DSTs (n = 3,483) was partially concordant with the CC assignment, however, an absence of bootstrap threshold supported nodes or p-distance, and the lack of association with the other epidemiological variables, evidenced the limitations of the MLST scheme. Marked genetic admixture signals were identified by STRUCTURE, with the majority being attributable to recombination events according to the RDP program results, although another type of exchange event cannot be ruled out. Our results reaffirm the genetic diversity inherent in the genes used for the MLST scheme, which are associated with the chromosomal remodeling already proposed for C. albicans. This was also corroborated with an internal validation at a micro geographical scale. Despite these results are biased due to the unavailability of considering the broad global spectrum of C. albicans isolates around the world. This suggests that the strategy used to population type this pathogen should be reevaluated to improve epidemiological monitoring of its health impact. Copyright © 2019 Muñoz, Wintaco, Muñoz and Ramírez. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.3389/fmicb.2019.01052
dc.identifier.issn1664302X
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/24093
dc.language.isoengspa
dc.publisherFrontiers Media S.A.spa
dc.relation.citationIssueNo. MAY
dc.relation.citationTitleFrontiers in Microbiology
dc.relation.citationVolumeVol. 10
dc.relation.ispartofFrontiers in Microbiology, ISSN:1664302X, Vol.10, No.MAY (2019)spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85068621121&doi=10.3389%2ffmicb.2019.01052&partnerID=40&md5=ffc8f01414dec8836e01ebca2bc5a19bspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordAdultspa
dc.subject.keywordArticlespa
dc.subject.keywordBootstrappingspa
dc.subject.keywordCandida albicansspa
dc.subject.keywordChromosomespa
dc.subject.keywordControlled studyspa
dc.subject.keywordDiploidyspa
dc.subject.keywordEpidemiological monitoringspa
dc.subject.keywordFemalespa
dc.subject.keywordGenetic variabilityspa
dc.subject.keywordHousekeeping genespa
dc.subject.keywordHumanspa
dc.subject.keywordHuman experimentspa
dc.subject.keywordMajor clinical studyspa
dc.subject.keywordMolecular epidemiologyspa
dc.subject.keywordMultilocus sequence typingspa
dc.subject.keywordNonhumanspa
dc.subject.keywordPopulation genetic structurespa
dc.subject.keywordVaginaspa
dc.subject.keywordValidation processspa
dc.subject.keywordCandida albicansspa
dc.subject.keywordMolecular epidemiologyspa
dc.subject.keywordMultilocus sequence typingspa
dc.subject.keywordPopulation genetic structurespa
dc.subject.keywordRecombinationspa
dc.titleDissecting the Heterogeneous Population Genetic Structure of Candida albicans: Limitations and constraints of the multilocus sequence typing schemespa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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