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Distinctive adaptive response to repeated exposure to hydrogen peroxide associated with upregulation of DNA repair genes and cell cycle arrest

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dc.creatorSanta-Gonzalez, Gloria A.spa
dc.creatorGomez-Molina, Andreaspa
dc.creatorArcos-Burgos, Mauriciospa
dc.creatorMeyer, Joel N.spa
dc.creatorCamargo, Mauriciospa
dc.date.accessioned2020-08-19T14:42:36Z
dc.date.available2020-08-19T14:42:36Z
dc.date.created2016-10spa
dc.description.abstractMany environmental and physiological stresses are chronic. Thus, cells are constantly exposed to diverse types of genotoxic insults that challenge genome stability, including those that induce oxidative DNA damage. However, most in vitro studies that model cellular response to oxidative stressors employ short exposures and/or acute stress models. In this study, we tested the hypothesis that chronic and repeated exposure to a micromolar concentration of hydrogen peroxide (H2O2) could activate DNA damage responses, resulting in cellular adaptations. For this purpose, we developed an in vitro model in which we incubated mouse myoblast cells with a steady concentration of ~50 ?M H2O2 for one hour daily for seven days, followed by a final challenge of a 10 or 20X higher dose of H2O2 (0.5 or 1 mM). We report that intermittent long-term exposure to this oxidative stimulus nearly eliminated cell toxicity and significantly decreased genotoxicity (in particular, a >5-fold decreased in double-strand breaks) resulting from subsequent acute exposure to oxidative stress. This protection was associated with cell cycle arrest in G2/M and induction of expression of nine DNA repair genes. Together, this evidence supports an adaptive response to chronic, low-level oxidative stress that results in genomic protection and up-regulated maintenance of cellular homeostasis.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.redox.2016.07.004
dc.identifier.issnISSN: 2213-2317
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/27532
dc.language.isoengspa
dc.publisherElsevierspa
dc.relation.citationEndPage133
dc.relation.citationStartPage124
dc.relation.citationTitleRedox Biology
dc.relation.citationVolumeVol. 9
dc.relation.ispartofRedox Biology, ISSN: 2213-2317, Vol.9 (2016); pp. 124-133 spa
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S2213231716300751?via%3Dihubspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.sourceRedox Biologyspa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.keywordROSspa
dc.subject.keywordGenotoxicityspa
dc.subject.keywordDNA damage responsespa
dc.subject.keywordUp-regulation of DNA repair genesspa
dc.subject.keywordG2/M arrestspa
dc.subject.keywordAdaptationspa
dc.titleDistinctive adaptive response to repeated exposure to hydrogen peroxide associated with upregulation of DNA repair genes and cell cycle arrestspa
dc.title.TranslatedTitleRespuesta adaptativa distintiva a la exposición repetida al peróxido de hidrógeno asociada con la regulación positiva de los genes de reparación del ADN y la detención del ciclo celularspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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