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Cross-reactivity between annexin A2 and Beta-2-glycoprotein I in animal models of antiphospholipid syndrome

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dc.creatorWeiss R.spa
dc.creatorBitton A.spa
dc.creatorNahary L.spa
dc.creatorArango M.T.spa
dc.creatorBenhar I.spa
dc.creatorBlank M.spa
dc.creatorShoenfeld Y.spa
dc.creatorChapman J.spa
dc.date.accessioned2020-05-26T00:11:04Z
dc.date.available2020-05-26T00:11:04Z
dc.date.created2017spa
dc.description.abstractAntiphospholipid syndrome (APS) affects coagulation and the brain by autoimmune mechanisms. The major antigen in APS is beta-2-glycoprotein I (?2-GPI) is known to complex with annexin A2 (ANXA2), and antibodies to ANXA2 have been described in APS. We measured these antibodies in mice with experimental APS (eAPS) induced by immunization with ?2-GPI. Sera of these mice reacted significantly with recombinant ANXA2 by enzyme-linked immunosorbent assay (ELISA) and the eAPS mice had significantly high levels of immunoglobulin G (IgG) in the brain by immunoblot assays compared to adjuvant immunized controls. Immunoprecipitation performed by mixing eAPS brain tissue with protein-G beads resulted in identification of two autoantigens unique to the eAPS group, one of which was ANXA2. In order to study more directly and methodically the specific role of anti-ANXA2 antibodies in APS, we immunized mice with ?2-GPI which contained no ANXA2 or with ANXA2 and measured antibodies to these proteins. Levels of antibodies to ANXA2 measured by ELISA were 0.72 ± 0.007 arbitrary units (a.u), 0.24 ± 0.03 and 0.02 ± 0.01 a.u for sera from ANXA2, ?2-GPI and control mice, respectively (p  less than  0.0001 and p = 0.037 for the comparison of the ANXA2 and ?2-GPI groups to the controls). Purified IgG from ?2-GPI sera did not show cross-binding with ANXA2. Antibodies to ?2-GPI and phospholipids were found in the ?2-GPI immunized group only. The present study suggests an immune response to the ?2-GPI–ANXA2 complex in eAPS and provides a novel ANXA2 immunization model which will serve to study the role of ANXA2 antibodies in of APS. © 2016, Springer Science+Business Media New York.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1007/s12026-016-8840-8
dc.identifier.issn0257277X
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/24279
dc.language.isoengspa
dc.publisherHumana Press Inc.spa
dc.relation.citationEndPage362
dc.relation.citationIssueNo. 1
dc.relation.citationStartPage355
dc.relation.citationTitleImmunologic Research
dc.relation.citationVolumeVol. 65
dc.relation.ispartofImmunologic Research, ISSN:0257277X, Vol.65, No.1 (2017); pp. 355-362spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84979633320&doi=10.1007%2fs12026-016-8840-8&partnerID=40&md5=86b79ce9fd3350ff51b86519d6ba39d7spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordBeta2 glycoprotein 1spa
dc.subject.keywordinbred balb ceng
dc.subject.keywordLipocortin 2spa
dc.subject.keywordBeta2 glycoprotein 1spa
dc.subject.keywordImmunoglobulin gspa
dc.subject.keywordLipocortin 2spa
dc.subject.keywordPhospholipid antibodyspa
dc.subject.keywordAnimal experimentspa
dc.subject.keywordAnimal modelspa
dc.subject.keywordAnimal tissuespa
dc.subject.keywordAntiphospholipid syndromespa
dc.subject.keywordArticlespa
dc.subject.keywordChemoluminescencespa
dc.subject.keywordControlled studyspa
dc.subject.keywordCross reactionspa
dc.subject.keywordEnzyme linked immunosorbent assayspa
dc.subject.keywordFemalespa
dc.subject.keywordImmobilized metal affinity chromatographyspa
dc.subject.keywordImmune responsespa
dc.subject.keywordImmunizationspa
dc.subject.keywordImmunoblottingspa
dc.subject.keywordImmunoprecipitationspa
dc.subject.keywordMousespa
dc.subject.keywordNonhumanspa
dc.subject.keywordPriority journalspa
dc.subject.keywordProtein expressionspa
dc.subject.keywordRadioimmunoprecipitationspa
dc.subject.keywordAnimalspa
dc.subject.keywordAntiphospholipid syndromespa
dc.subject.keywordBagg albino mousespa
dc.subject.keywordBloodspa
dc.subject.keywordBrainspa
dc.subject.keywordCross reactionspa
dc.subject.keywordDisease modelspa
dc.subject.keywordImmunologyspa
dc.subject.keywordAnimalsspa
dc.subject.keywordAnnexin a2spa
dc.subject.keywordAntibodieseng
dc.subject.keywordAntiphospholipid syndromespa
dc.subject.keywordBeta 2-glycoprotein ispa
dc.subject.keywordBrainspa
dc.subject.keywordCross reactionsspa
dc.subject.keywordDisease modelseng
dc.subject.keywordEnzyme-linked immunosorbent assayspa
dc.subject.keywordFemalespa
dc.subject.keywordImmunoglobulin gspa
dc.subject.keywordMiceeng
dc.subject.keywordAnnexinspa
dc.subject.keywordAnticardiolipinspa
dc.subject.keywordAntiphospholipid syndromespa
dc.subject.keywordAutoantibodiesspa
dc.subject.keywordAutoimmunespa
dc.subject.keywordBeta-2-glycoproteinspa
dc.titleCross-reactivity between annexin A2 and Beta-2-glycoprotein I in animal models of antiphospholipid syndromespa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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