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Comparison of parasite loads in serum and blood samples from patients in acute and chronic phases of Chagas disease – CORRIGENDUM

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dc.creatorGalindo Hernández, Carolinaspa
dc.creatorTeherán, Aníbalspa
dc.creatorFlórez, Carolinaspa
dc.creatorRamírez, Juan Davidspa
dc.date.accessioned2020-08-06T16:21:28Z
dc.date.available2020-08-06T16:21:28Z
dc.date.created2018-12spa
dc.description.abstractMolecular methods have been developed for the detection and quantification of Trypanosoma cruzi DNA in blood samples from patients with Chagas disease. However, aspects of sample processing necessary for quantitative real-time PCR (qPCR), such as the addition of guanidine hydrochloride to whole blood samples, may limit timely access to molecular diagnosis. We analysed 169 samples from serum and guanidine-EDTA blood (GEB) obtained from patients in acute and chronic phases of Chagas disease. We applied qPCR targeted to the satellite DNA region. Finally, we compared the parasite loads and cycle of threshold values of the qPCR. The results confirmed the usefulness of serum samples for the detection and quantification of parasite DNA in patients with Chagas disease, especially in the acute phase. However, the parasite loads detected in serum samples from patients in the chronic phase were lower than those detected in GEB samples. The epidemiological implications of the findings are herein discussed. eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1017/s0031182018000847
dc.identifier.issnISSN: 0031-1820
dc.identifier.issnEISSN: 1469-8161
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/26370
dc.language.isoengspa
dc.publisherCambridge University Pressspa
dc.relation.citationEndPage1844
dc.relation.citationIssueNo. 14
dc.relation.citationStartPage1837
dc.relation.citationTitleParasitology
dc.relation.citationVolumeVol. 145
dc.relation.ispartofParasitology, ISSN: ISSN: 0031-1820 ; EISSN: 1469-8161, Vol.145, No.14 (2018-12); pp.1837-1844spa
dc.relation.urihttps://www.cambridge.org/core/services/aop-cambridge-core/content/view/AE1C2EF86FD8C30961500E67DDBE6326/S0031182018000847a.pdf/div-class-title-comparison-of-parasite-loads-in-serum-and-blood-samples-from-patients-in-acute-and-chronic-phases-of-chagas-disease-corrigendum-div.pdfspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.sourceParasitologyspa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.keywordAgricultural and Veterinary Sciencesspa
dc.subject.keywordVeterinary Sciencesspa
dc.titleComparison of parasite loads in serum and blood samples from patients in acute and chronic phases of Chagas disease – CORRIGENDUMspa
dc.title.TranslatedTitleComparación de las cargas de parásitos en muestras de suero y sangre de pacientes en fases agudas y crónicas de la enfermedad de Chagas - CORRIGENDUMspa
dc.typeothereng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaErrataspa
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