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Mycobacterium tuberculosis surface protein Rv0227c contains high activity binding peptides which inhibit cell invasion

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dc.creatorRodríguez, Diana Marcelaspa
dc.creatorOcampo, Marisol
dc.creatorCurtidor, Hernandospa
dc.creatorVanegas, Magnoliaspa
dc.creatorPatarroyo, Manuel Elkinspa
dc.creatorPatarroyo, Manuel A.
dc.date.accessioned2020-05-25T23:58:03Z
dc.date.available2020-05-25T23:58:03Z
dc.date.created2012spa
dc.description.abstractMycobacterium tuberculosis surface proteins involved in target cell invasion may be identified as a strategy for developing subunit-based, chemically-synthesized vaccines. The Rv0227c protein was thus selected to assess its role in the invasion and infection of Mycobacterium tuberculosis target cells. Results revealed Rv0227c localization on mycobacterial surface by immunoelectron microscopy and Western blot. Receptor-ligand assays using 20-mer, non-overlapping peptides covering the complete Rv0227c protein sequence revealed three high activity binding peptides for U937 phagocytic cells and seven for A549 cells. Peptide 16944 significantly inhibited mycobacterial entry to both cell lines while 16943 and 16949 only managed to inhibit entrance to U937 cells and 16951 to A549 cells. The Jnet bioinformatics tool predicted secondary structure elements for the complete protein, agreeing with elements determined for such chemically-synthesized peptides. It was thus concluded that high activity binding peptides which were able to inhibit mycobacterial entry to target cells are of great importance when selecting peptide candidates for inclusion in an anti-tuberculosis vaccine. © 2012 Elsevier Inc.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.peptides.2012.08.023
dc.identifier.issn1969781
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22792
dc.language.isoengspa
dc.relation.citationEndPage216
dc.relation.citationIssueNo. 2
dc.relation.citationStartPage208
dc.relation.citationTitlePeptides
dc.relation.citationVolumeVol. 38
dc.relation.ispartofPeptides, ISSN:1969781, Vol.38, No.2 (2012); pp. 208-216spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84868693669&doi=10.1016%2fj.peptides.2012.08.023&partnerID=40&md5=908dd73b50c6339f2c3e7a5348769bcdspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordBacterial proteinspa
dc.subject.keywordwesterneng
dc.subject.keywordProtein rv0227cspa
dc.subject.keywordUnclassified drugspa
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordAnimal experimentspa
dc.subject.keywordAntibacterial activityspa
dc.subject.keywordArticlespa
dc.subject.keywordBinding affinityspa
dc.subject.keywordBioinformaticsspa
dc.subject.keywordCell invasionspa
dc.subject.keywordConcentration responsespa
dc.subject.keywordControlled studyspa
dc.subject.keywordCytotoxicityspa
dc.subject.keywordExperimental rabbitspa
dc.subject.keywordImmunoelectron microscopyspa
dc.subject.keywordNonhumanspa
dc.subject.keywordPhagocytespa
dc.subject.keywordPolymerase chain reactionspa
dc.subject.keywordPriority journalspa
dc.subject.keywordProtein analysisspa
dc.subject.keywordProtein bindingspa
dc.subject.keywordProtein localizationspa
dc.subject.keywordSpectroscopyspa
dc.subject.keywordTuberculosisspa
dc.subject.keywordWestern blottingspa
dc.subject.keywordBacterial proteinsspa
dc.subject.keywordBacterial vaccinesspa
dc.subject.keywordBlottingeng
dc.subject.keywordCell survivalspa
dc.subject.keywordDose-response relationshipeng
dc.subject.keywordHumansspa
dc.subject.keywordMembrane proteinsspa
dc.subject.keywordMicroscopyeng
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordPeptidesspa
dc.subject.keywordReverse transcriptase polymerase chain reactionspa
dc.subject.keywordStructure-activity relationshipspa
dc.subject.keywordTumor cellseng
dc.subject.keywordU937 cellsspa
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordInvasionspa
dc.subject.keywordMycobacterium tuberculosisspa
dc.subject.keywordReceptor-ligand interactionspa
dc.subject.keywordRv0227c proteinspa
dc.subject.keywordSynthetic peptidespa
dc.subject.keywordVaccinespa
dc.titleMycobacterium tuberculosis surface protein Rv0227c contains high activity binding peptides which inhibit cell invasionspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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