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Sexual forms obtained in a continuous in vitro cultured Colombian strain of Plasmodium falciparum (FCB2)

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dc.creatorArarat-Sarria, Monicaspa
dc.creatorPrado, Cesar Camilospa
dc.creatorCamargo, Milenaspa
dc.creatorOspina, Laura Tatianaspa
dc.creatorCamargo, Paola Andreaspa
dc.creatorCurtidor, Hernandospa
dc.creatorPatarroyo, Manuel A.
dc.date.accessioned2020-05-25T23:56:53Z
dc.date.available2020-05-25T23:56:53Z
dc.date.created2020spa
dc.description.abstractBackground: The epidemiological control of malaria has been hampered by the appearance of parasite resistance to anti-malarial drugs and by the resistance of mosquito vectors to control measures. This has also been associated with weak transmission control, mostly due to poor control of asymptomatic patients associated with host-vector transmission. This highlights the importance of studying the parasite's sexual forms (gametocytes) which are involved in this phase of the parasite's life-cycle. Some African and Asian strains of Plasmodium falciparum have been fully characterized regarding sexual forms' production; however, few Latin-American strains have been so characterized. This study was aimed at characterizing the Colombian FCB2 strain as a gametocyte producer able to infect mosquitoes. Methods: Gametocyte production was induced in in vitro cultured P. falciparum FCB2 and 3D7 strains. Pfap2g and Pfs25 gene expression was detected in FCB2 strain gametocyte culture by RT-PCR. Comparative analysis of gametocytes obtained from both strains was made (counts and morphological changes). In vitro zygote formation from FCB2 gametocytes was induced by incubating a gametocyte culture sample at 27 °C for 20 min. A controlled Anopheles albimanus infection was made using an artificial feed system with cultured FCB2 gametocytes (14-15 days old). Mosquito midgut dissection was then carried out for analyzing oocysts. Results: The FCB2 strain expressed Pfap2g, Pfs16, Pfg27/25 and Pfs25 sexual differentiation-related genes after in vitro sexual differentiation induction, producing gametocytes that conserved the expected morphological features. The amount of FCB2 gametocytes produced was similar to that from the 3D7 strain. FCB2 gametocytes were differentiated into zygotes and ookinetes after an in vitro low-temperature stimulus and infected An. albimanus mosquitoes, developing to oocyst stage. Conclusions: Even with the history of long-term FCB2 strain in vitro culture maintenance, it has retained its sexual differentiation ability. The gametocytes produced here preserved these parasite forms' usual characteristics and An. albimanus infection capability, thus enabling its use as a tool for studying sexual form biology, An. albimanus infection comparative analysis and anti-malarial drug and vaccine development. © 2020 The Author(s).eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1186/s12936-020-3142-y
dc.identifier.issn14752875
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22550
dc.language.isoengspa
dc.publisherBioMed Central Ltd.spa
dc.relation.citationIssueNo. 1
dc.relation.citationTitleMalaria Journal
dc.relation.citationVolumeVol. 19
dc.relation.ispartofMalaria Journal, ISSN:14752875, Vol.19, No.1 (2020)spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85078940120&doi=10.1186%2fs12936-020-3142-y&partnerID=40&md5=aa01712b42fe88274f26e714417ba069spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordAntimalarial agentspa
dc.subject.keywordMalaria vaccinespa
dc.subject.keywordAnimal cellspa
dc.subject.keywordAnophelesspa
dc.subject.keywordAnopheles albimanusspa
dc.subject.keywordAnopheles albimanus infectionspa
dc.subject.keywordArticlespa
dc.subject.keywordColombiaspa
dc.subject.keywordComparative studyspa
dc.subject.keywordControlled studyspa
dc.subject.keywordErythrocytespa
dc.subject.keywordFemalespa
dc.subject.keywordGametocytespa
dc.subject.keywordGenespa
dc.subject.keywordGene expressionspa
dc.subject.keywordGenetic analysisspa
dc.subject.keywordHumanspa
dc.subject.keywordHuman cellspa
dc.subject.keywordIn vitro studyspa
dc.subject.keywordIncubation timespa
dc.subject.keywordInfectionspa
dc.subject.keywordLow temperaturespa
dc.subject.keywordMicrobial morphologyspa
dc.subject.keywordMidgutspa
dc.subject.keywordNonhumanspa
dc.subject.keywordOocystspa
dc.subject.keywordParasite examinationspa
dc.subject.keywordParasite transmissionspa
dc.subject.keywordPfap2g genespa
dc.subject.keywordPfg25 genespa
dc.subject.keywordPfg27 genespa
dc.subject.keywordPfs16 genespa
dc.subject.keywordPfs25 genespa
dc.subject.keywordPlasmodium falciparumspa
dc.subject.keywordPlasmodium falciparum 3d7spa
dc.subject.keywordPlasmodium falciparum fcb2spa
dc.subject.keywordReal time polymerase chain reactionspa
dc.subject.keywordSex differentiationspa
dc.subject.keywordStimulusspa
dc.subject.keywordFcb2spa
dc.subject.keywordGametocytespa
dc.subject.keywordMalariaspa
dc.subject.keywordMosquito infectivityspa
dc.subject.keywordOocystspa
dc.subject.keywordPlasmodium falciparumspa
dc.subject.keywordSexual differentiationspa
dc.titleSexual forms obtained in a continuous in vitro cultured Colombian strain of Plasmodium falciparum (FCB2)spa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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