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The identification of two Trypanosoma cruzi I genotypes from domestic and sylvatic transmission cycles in Colombia based on a single polymerase chain reaction amplification of the spliced-leader intergenic region

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dc.creatorVilla, Lina Marcelaspa
dc.creatorGuhl, Felipespa
dc.creatorZabala, Danielspa
dc.creatorRamírez, Juan Davidspa
dc.creatorUrrea, Daniel Alfonsospa
dc.creatorHernández, Diana Carolinaspa
dc.creatorCucunubá, Zulmaspa
dc.creatorMontilla, Marlenyspa
dc.creatorCarranza, Julio Césarspa
dc.creatorRueda, Karinaspa
dc.creatorTrujillo, Jorge Eduardospa
dc.creatorVallejo, Gustavo Adolfospa
dc.date.accessioned2020-08-19T14:40:56Z
dc.date.available2020-08-19T14:40:56Z
dc.date.created2013-11spa
dc.description.abstractA single polymerase chain reaction (PCR) reaction targeting the spliced-leader intergenic region of Trypanosoma cruzi I was standardised by amplifying a 231 bp fragment in domestic (TcIDOM) strains or clones and 450 and 550 bp fragments in sylvatic strains or clones. This reaction was validated using 44 blind coded samples and 184 non-coded T. cruzi I clones isolated from sylvatic triatomines and the correspondence between the amplified fragments and their domestic or sylvatic origin was determined. Six of the nine strains isolated from acute cases suspected of oral infection had the sylvatic T. cruzi I profile. These results confirmed that the sylvatic T. cruzi I genotype is linked to cases of oral Chagas disease in Colombia. We therefore propose the use of this novel PCR reaction in strains or clones previously characterised as T. cruzi I to distinguish TcIDOMfrom sylvatic genotypes in studies of transmission dynamics, including the verification of population selection within hosts or detection of the frequency of mixed infections by both T. cruzi I genotypes in Colombia.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1590/0074-0276130201
dc.identifier.issnISSN: 0074-0276
dc.identifier.issnEISSN: 1678-8060
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/27076
dc.language.isoengspa
dc.publisherInstituto Oswaldo Cruzspa
dc.relation.citationEndPage935
dc.relation.citationIssueNo. 7
dc.relation.citationStartPage932
dc.relation.citationTitleMemorias do Instituto Oswaldo Cruz
dc.relation.citationVolumeVol. 108
dc.relation.ispartofMemorias do Instituto Oswaldo Cruz, ISSN: 0074-0276;EISSN: 1678-8060, Vol.108, No.7 (Nov 2013); pp. 932-935spa
dc.relation.urihttps://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000700932&lng=en&tlng=enspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.sourceMemorias do Instituto Oswaldo Cruzspa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.keywordSpliced-leader intergenic regionspa
dc.subject.keywordTrypanosoma cruzi Ispa
dc.subject.keywordPCRspa
dc.subject.keywordChagas diseasespa
dc.subject.keywordDomestic cyclespa
dc.subject.keywordSylvatic cyclespa
dc.titleThe identification of two Trypanosoma cruzi I genotypes from domestic and sylvatic transmission cycles in Colombia based on a single polymerase chain reaction amplification of the spliced-leader intergenic regionspa
dc.title.TranslatedTitleLa identificación de dos genotipos de Trypanosoma cruzi I de ciclos de transmisión domésticos y silvestres en Colombia basados ??en la amplificación de una sola reacción en cadena de la polimerasa de la región intergénica del líder empalmadospa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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