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Activation of the PI3K/AKT signalling pathway by insulin-like growth factor type II stimulates metalloproteinase 9 mRNA expression in choriocarcinoma cells

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dc.creatorPinzón M.L.spa
dc.creatorDíaz L.E.spa
dc.creatorOrtiz B.L.spa
dc.creatorUmaña A.spa
dc.creatorde Rodríguez S.C.spa
dc.creatorde Gómez M.S.spa
dc.date.accessioned2020-05-25T23:59:32Z
dc.date.available2020-05-25T23:59:32Z
dc.date.created2009spa
dc.description.abstractMetalloproteinases 2 and 9 (MMP-2 and MMP-9) and Tissue Inhibitor-1 of MMPs (TIMP-1) could contribute to regulate the invasive behaviour of trophoblastic cells, effect which could be mediated by Insulin- like growth factor type II (IGF-II), which regulates the development and function of trophoblast at the foetal-maternal interface. The aim of this study was to investigate the role of the PI3K/AKT signalling pathway activated by IGF-II in the expression of MMP-2 and MMP-9 involved in the invasion process in the human choriocarcinoma cell line JEG-3. MMP-2, MMP-9 and TIMP-1mARNexpression in those cells were evaluated by RT-PCR using different IGF-II doses and conditions while signalling pathway was evaluated using Western blot. It was found that IGF-II does not contribute to trophoblastic cells proliferation, however, it promotes mARN expression of MMP-9 and TIMP-1, but not of MMP-2, in a dose-dependent way. The effect on MMP-9 expression is mediated through IGF-II activation of PI3K/AKT signaling pathway after phosphorylation of the IGF-I receptor (IGF-IR). According with the results a model is proposed in which the interaction of IGF-II with IGF-IR leads to PI3K/AKT activation and subsequent expression and activation of MMP-9. This activation is an essential requirement for the invasive process.spa
dc.format.mimetypeapplication/pdf
dc.identifier.issn1202804
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23062
dc.language.isospaspa
dc.relation.citationEndPage362
dc.relation.citationIssueNo. 3
dc.relation.citationStartPage341
dc.relation.citationTitleRevista Colombiana de Quimica
dc.relation.citationVolumeVol. 38
dc.relation.ispartofRevista Colombiana de Quimica, ISSN:1202804, Vol.38, No.3 (2009); pp. 341-362spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-77956979925&partnerID=40&md5=740e5ae6caece060e494f4a2bf2b25b7spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordInvasionspa
dc.subject.keywordMetalloproteinasesspa
dc.subject.keywordPI3K/AKTspa
dc.subject.keywordTrophoblastspa
dc.titleActivation of the PI3K/AKT signalling pathway by insulin-like growth factor type II stimulates metalloproteinase 9 mRNA expression in choriocarcinoma cellsspa
dc.title.TranslatedTitleLa activación de la vía de señalización PI3K/AKT por el factor de crecimiento similar a la insulina tipo II estimula la expresión del mARN de la metaloproteinasa 9 en células de coriocarcinomspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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