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In vitro activity of the alkaloids mixture of Ervatamia coronaria (Jacq) Staff. Apocynaceae on Leishmania braziliensis amastigotes

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dc.creatorRodríguez, Amanda Morenospa
dc.creatorCamargo, Jorge Roblesspa
dc.creatorGarcía, Felio J. Bellospa
dc.date.accessioned2020-05-26T00:03:33Z
dc.date.available2020-05-26T00:03:33Z
dc.date.created2008spa
dc.description.abstractLeishmaniasis, caused by Leishmania sp., is one of the mean reason of considerable mortality and morbidity throughout the world, especially in the tropics. Cutaneous and mucocutaneous manifestations are caused by Leishmania braziliensis, and the cutaneous form is the most common one in Colombia. In the search for antileishmanial compounds from natural sources, we studied the alkaloids mixture from Ervatamia coronaria against L. braziliensis at six different concentrations (1.0, 10, 20, 25, 50 and 100 ?g/mL). Macrophages J774 infected with L. braziliensis were treated with alkaloids one hour, and once a day for three days, after parasitic infection and preserving the same culture medium. Cytotoxicity with trypan blue was undertaken in macrophages J774 by using the same concentrations. Three different cultures samples were carried out. As a control we used medium alone. The alkaloids mix showed a dose/dependent activity on amastigote, but by increasing concentrations from 50 to 100 ?g/mL for three days, we saw a high index of infection, probably caused by cellular death. We did not see any toxic effect on macrophages J774 at 100 ?g/mL, LD50/24h= 233.52 ?g/mL. These results revealed a novel pharmacological activity of alkaloids from E. coronaria against amastigotes of L. braziliensis IC50 = 2.6 and 12.4 ?g/mL without toxicity on host cells.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1590/S0102-695X2008000300007
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23605
dc.language.isospaspa
dc.relation.citationEndPage355
dc.relation.citationIssueNo. 3
dc.relation.citationStartPage350
dc.relation.citationTitleBrazilian Journal of Pharmacognosy
dc.relation.citationVolumeVol. 18
dc.relation.ispartofBrazilian Journal of Pharmacognosy, Vol.18, No.3 (2008); pp. 350-355spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-55849092895&doi=10.1590%2fS0102-695X2008000300007&partnerID=40&md5=53b4b2449d899f43152ac2624281c3aaspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordAlkaloidsspa
dc.subject.keywordAmastigotespa
dc.subject.keywordApocynaceaespa
dc.subject.keywordCytotoxicityspa
dc.subject.keywordErvatamia coronariaspa
dc.subject.keywordLeishmaniaspa
dc.titleIn vitro activity of the alkaloids mixture of Ervatamia coronaria (Jacq) Staff. Apocynaceae on Leishmania braziliensis amastigotesspa
dc.title.TranslatedTitleActividad in vitro de la mezcla de alcaloides de Ervatamia coronaria (Jacq) Staff. Apocynaceae sobre amastigotes de Leishmania braziliensiseng
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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